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Transcriptomic analysis in Anemone flaccida rhizomes reveals ancillary pathway for triterpene saponins biosynthesis and differential responsiveness to phytohormones

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Chinese Journal of Natural Medicines 2019年第2期17卷 131-144页

作者： MO Guo-Yan HUANG Fang FANG Yin HAN Lin-Tao Kayla K.Pennerman BU Li-Jing DU Xiao-Wei Joan W.Bennett YIN Guo-Hua China Key Laboratory of TCM Resource and Prescription Ministry of EducationHubei University of Chinese Medicine Department of Plant Biology and Pathology RutgersThe State University of New Jersey Department of Biology University of New Mexico Department of Pharmacy Heilongjiang University of Chinese Medicine

Anemone flaccida fr. schmidt is a perennial medicinal herb that contains pentacyclic triterpenoid saponins as the major bioactive constituents. In China, the rhizomes are used as treatments for a variety of ailments including arthritis. However, yields of the saponins are low, and little is known about the plant's genetic background or phytohormonal responsiveness. Using one-quarter of the 454 pyrosequencing information from the Roche GS FLX Titanium platform, we performed a transcriptomic analysis to identify 157 genes putatively encoding 26 enzymes involved in the synthesis of the bioactive compounds. It was revealed that there are two biosynthetic pathways of triterpene saponins in A. flaccida. One pathway depends on β-amyrin synthase and is similar to that found in other plants. The second, subsidiary("backburner") pathway is catalyzed by camelliol C synthase and yields β-amyrin as minor byproduct. Both pathways used cytochrome P450-dependent monooxygenases(CYPs) and family 1 uridine diphosphate glycosyltransferases(UGTs) to modify the triterpenoid backbone. The expression of CYPs and UGTs were quite different in roots treated with the phytohormones methyl jasmonate, salicylic acid and indole-3-acetic acid. This study provides the first large-scale transcriptional dataset for the biosynthetic pathways of triterpene saponins and their phytohormonal responsiveness in the genus Anemone.

关键词： Anemone flaccida fr.schmidt Triterpenoid saponins Biosynthetic pathways Transcriptomic analysis Phytohormonal responsiveness

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