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Activity of chlorpromazine on nfa1 and Mp2CL5 genes of Naegleria fowleri trophozoites

Health 2011年第3期3卷 166-171页

作者： Supathra Tiewcharoen Jundee Rabablert Prucksawan Chetannachan Dusit Worawirunwong Virach Junnu Noppamas Pungsub 不详

Amoeba treatment of patients suffering from pri-mary amoebic meningoencephalitis caused by Naegleria fowleri has not been successful. Dam-aged morphology and effect on genes of N. fowleri as the result of its initial interaction with drug may provide clue to the success of treatment. In this study, we investigated the activity of chlorpromazine compared with amphotericin B and voriconazole against *** Khon Kaen strain using cell based assay and molecular techniques. Scanning electron and light micro-graph showed the drug interaction of treated amoebae with 0.098 ug/ml chlorpromazine was faster than 0.002 ug/ml amphotericin B and 12.5 ug/ml of voriconazole. The morphological cha-racteristics of treated amoebae with Gomori’s trichrome stain correlated to the scanning elec-tron microscope study. The effect of drugs to nfa1 and Mp2CL5 genes of treated amoebae found that at 120 min post exposure, chlorpromazine, voriconazole inhibited both genes except amphotericin B. Most of drug inhibited nfa1 except fluconazole. The results evaluated that chlorpromazine was higher potency and rapidly activity than amphotericin B and voriconazole against N. fowleri trophozoites.

关键词： Naegleria fowleri Chlorpromazine Scanning Micrograph Gene Expression

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ITS and pB2.5 gene expression of Naegleria fowleri in drug resistance

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Health 2011年第8期3卷 529-533页

作者： Jundee Rabablert Supathra Tiewcharoen Virach Junnu Department of Parasitology Faculty of Medicine Siriraj Hospital Mahidol University Bangkok Thailand

Naegleria fowleri was causative agent of primary amoebic meningoencephalitis (PAM). Accroding to the failure of treatment, several researches reported the activity of chemotherapeutic drugs against *** but we did not know the drug resistance of the amoebae. The purpose of this study was to examine the effects of drugs (amphotericin B, artesunate, azithromycin, voriconazole, chlorpromazine, fluconazole and gentamicin sulphate) on ITS and pB2.3 genes of Naegleria fowleri trophozoites. Our study demonstrated gene expression of treated *** by RT-PCR. The results reviewed that ITS gene of N. fowleri showed up regulate to amphotericin B, azithromicin and gentamicin sulphate, while pB2.3 gene showed up regulate to artesunate. These results compared with beta actin (house keeping gene) expression at time intervals 15 - 120 min. The change of gene expression of treated *** was possibly to cause of drug resistance. The mechanism of drug resistance genes ITS and pB2.3 of *** should be clarified in further study.

关键词： Naegleria fowleri ITS pB2.3 Drug Resistance

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