检索结果-南通市图书馆

In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment

引用

Chinese Chemical Letters 2023年第12期34卷 452-455页

作者： Haocheng Gong Yanhui Zhang Ying Gao Xinrong Tian Pengzhao Wu Xiaoyu Wei Yuan Guo Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry and Materials ScienceNorthwest UniversityXi'an710127China

We here present a Förster resonance energy transfer(FRET)-based and environment-sensitive fluorescent probe VG-1 for vicinal-dithiol-containing proteins(VDPS).VG-1 uniquely contalns two sites sensitive to the protein environment(SPE)thus it shows weak fluorescence in both blue and green channels(a low FRET efficiency)in *** specifically binding with VDPs,its fuorescence in the green channel increases,while that in the blue channel disappears,achieving the specific detection of *** obvioussignal changes in fluorescence may be attributed to that the increased rigidity of the molecular skeletons causes the enhanced FRET eficiency,The probe also achleved the cell super-resolution imaging of VDPs and the confocal imaging of VDPs in zebrafish.

关键词： Vicinal-dithiol-containing proteins Environment sensitivity Super-resolution imaging Fluorescent probes forster resonance energy transfer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A versatile platform for single-molecule enzymology of restriction endonuclease

引用

Journal of Innovative Optical Health Sciences 2019年第1期12卷 29-38页

作者： Xin Wang Jingyuan Nie Yi Li Hai Pan Peng Zheng Meng Qin Yi Cao Wei Wang National Laboratory of Solid State Microstructure School of Physics Nanjing University 22 Hankou Road NanjingJiangsu 210093P.R.China School of Chemistry and Chemical Engineering Nanjing University 163 Xianlin Avenue Nanjing Jiangsu 210023P.R.China

Enzymes are the major players for many biological *** studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble ***,these single-molecule experiments are technically di±cult and generally require complicated experimental ***,we develop a Holliday junction(HJ)-based platform to study the activity of restriction endonucleases at the single-molecule level using single-molecule FRET(sm-FRET).We show that the intrinsic dynamics of HJ can be used as the reporter for both the enzyme-binding and the substrate-release *** to the multiple-arms structure of HJ,the fluorophore-labeled arms can be different from the surface anchoring arm and the substrate ***,it is possible to independently change the substrate arm to study different enzymes with similar *** a design is extremely useful for the systematic study of enzymes from the same family or enzymes bearing different pathologic ***,this method can be easily extended to study other types of DNA-binding enzymes without too much modi fication of the *** anticipate it can find broad applications in single-molecule enzymology.

关键词： Single molecule forster resonance energy transfer enzymology kinetics holliday junction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging

引用

Chinese Chemical Letters 2024年第9期35卷 183-186页

作者： Zhixue Liu Haiqi Chen Lijuan Guo Xinyao Sun Zhi-Yuan Zhang Junyi Chen Ming Dong Chunju Li Tianjin Key Laboratory of Structure and Performance for Functional Molecules College of ChemistryTianjin Normal UniversityTianjin 300387China

Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and ***,we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate(TP[3]AS)and tetraphenylethylene pyridinium(TPE-4Py)through electrostatic interactions,host-guest encapsulation andπ-πstacking interactions.F?rster resonance energy transfer(FRET)process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%,accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS *** contrast,other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic *** to the strong fluorescence and good stability of TPE-4Py@TP[3]AS,it can be used for optical imaging in living cells,which provided an effective approach for the construction of assembling-confined luminescent biomaterials.

关键词： Luminescent macrocycle Terphenarene sulfate Multicharged assembly Multivalent interactions forster resonance energy transfer Cell imaging

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

White Electroluminescence with Simultaneous Three-Color Emission from a Four-Armed Star-Shaped Single-Polymer System

引用

Chinese Journal of Chemistry 2015年第8期33卷 873-880页

作者： Yuanda Jiu Jianyun Wang Chengfang Liu Wenyong Lai Lingling Zhao Xiangchun Li Yi Jiang Weidong Xu Xinwen Zhang Wei Huang Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials Jiangsu National Synergetic Innovation Center for Advanced Materials Nanjing University of Posts & Telecommunications Nanjing Jiangsu 210023 China Key Laboratory of Flexible Electronics & Institute of Advanced Materials Jiangsu National Synergetie innovation Center for Advanced Materials Nanjing Tech University Nanjing Jiangsu 211816 China

A four-armed star-shaped single-polymer system with 4,7-bis（5-（4-（9H-carbazol-9-yl）phenyl）-4-hexylthio- phen-2-yl）benzo[c][1,2,5]thiadiazole （FTBT） as a red emissive core, polyfluorene （PF） as blue emissive arms and 1,3-benzo thiadiazole （BT） as green emissive dopants was designed and synthesized, in which red, green, and blue （RGB） emission balance can be achieved by adjusting the doping concentration of FTBT and BT discreetly. A typ- ical single-emissive-layer device （ITO/PEDOT：PSS/polymer/TPBI/LiF/Al） was studied and discussed, realizing a pure and stable white emission with a luminous efficiency （LE） of 1.59 cd·A^-1 and CIE coordinates of （0.31, 0.34）. The high-color-quality white electroluminescence of the devices could be mainly attributed to the suppressed in- termolecular interactions, and partial energy transfer from the blue PF arms to the red and green dopants.

关键词： white electroluminescence hyperbranched polymers multi-armed structures forster resonance energy transfer organic light-emitting diodes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：