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formin-like 3 regulates RhoC/FAK pathway and actin assembly to promote cell invasion in colorectal carcinoma

World Journal of Gastroenterology 2018年第34期24卷 3884-3897页

作者： Yuan-Feng Zeng Yi-Sheng Xiao Yong Liu Xiao-Jiang Luo Li-Dan Wen Qian Liu Min Chen Department of Pathology Jiangxi Provincial People’s Hospital Teaching and Researching Section of Morphology College of Basic Medicine Jiangxi University of Traditional Chinese Medicine Department of General Surgery Jiangxi Provincial People’s Hospital Clinical Medical Sciences Institute Jiangxi Provincial People’s Hospital

AIM To clarify the underlying mechanism of formin-like 3(FMNL3)in the promotion of colorectal carcinoma(CRC)cell *** The in vitro biological function analyses of FMNL3 were performed by gain-and loss-of function *** in the F-actin cytoskeleton were detected by the technologies of phalloidin-TRITC labeling and confocal *** signaling pathway mediated by FMNL3 was explored by western blot,gelatin zymograph assay,co-immunoprecipitation(co-IP),immunofluorescence colocalization,and glutathione S-transferase(GST)pulldown *** The in vitro experimental results showed that FMNL3 significantly promoted the proliferation,invasion,and migration of CRC cells(P<0.05 and P<0.01).Moreover,FMNL3regulated the remodeling of actin-based protrusions such as filopodia and lamellipodia in a RhoC-dependent *** western blot and gelatin zymograph assay results indicated that FMNL3 was involved in the RhoC/focal adhesion kinase(FAK)pathway and acted as an effector of RhoC to activate the downstream signaling of p-FAK as well as p-MAPK and *** resulted in the increased expression of matrix metalloproteinase 2(MMP2),matrix metalloproteinase 9(MMP9)and vascular endothelial growth factor(VEGF),and the subsequent promotion of CRC cell *** results of TAE226,U0126 or Ly294002 treatment confirmed an essential role of FMNL3 in activation of the RhoC/FAK pathway and the subsequent promotion of CRC ***-IP,colocalization and GST pull-down assays showed the direct interaction of FMNL3 with RhoC in vivo and in *** FMNL3 regulates the RhoC/FAK signaling pathway and RhoC-dependent remodeling of actin-based protrusions to promote CRC invasion.

关键词： formin-like 3 Colorectal carcinoma Invasion RhoC/FAK pathway Actin assembly

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Correlation of FMNL2 and HERC4 expression with the infiltrative growth of cancer cells in cervical cancer tissue

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Journal of Hainan Medical University 2017年第24期23卷 89-92页

作者： Qiu-Yuan Zhou Hong Li Hong-Li Wang Wei Li Yan-Hua Xu Department of Pathology the Central of Enshi Autonomous Prefecture Hubei ProvinceEnshiHubei Province445000

Objective: To study the correlation of FMNL2 and HERC4 expression with the infiltrative growth of cancer cells in cervical cancer tissue. Methods: Patients with cervical cancer who underwent surgical resection in our hospital between July 2014 and September 2016 were selected as the research subjects, the cervical cancer tissue and adjacent tissue were collected from the enrolled patients after operation to extract RNA, and PCR reaction kit was used to determine the mRNA expression of FMNL2, HERC4, epithelial mesenchymal transition genes and proliferation genes. Results: FMNL2, HERC4, Exosomes, Cripto-1, ZEB1, Snail, Vimentin, Piwil2, STOML-2, BCL-2, Smo and XIAP mRNA expression in cervical cancer tissue were significantly higher than those in adjacent tissue;Exosomes, Cripto-1, ZEB1, Snail and Vimentin mRNA expression in cervical cancer tissue with high FMNL2 expression were significantly higher than those in cervical cancer tissue with low FMNL2 expression;Piwil2, STOML-2, BCL-2, Smo and XIAP mRNA expression in cervical cancer tissue with high HERC4 expression were significantly higher than those in cervical cancer tissue with low HERC4 expression. Conclusion: The high expression of FMNL2 and HERC4 in cervical cancer tissue can promote the epithelial mesenchymal transition and proliferation of cancer cells respectively so as to participate in the infiltrative growth of cancer cells.

关键词： Cervical cancer formin-like 2 HERC4 Epithelial mesenchymal transition Proliferation

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