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Virologica Sinica 2020年第1期35卷 52-63页

作者： Jiadai Li Lingling Han Yao Hao Yuncong Yuan Mingzhen Wang Xiu Xin Hailong Wang Fang Yu Congyi Zheng Chao Shen State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China Department of Pathology Zhongnan HospitalWuhan UniversityWuhan 430072China China Center for Type Culture Collection Wuhan UniversityWuhan 430072China

foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication.

关键词： foot-and-mouth disease virus(FMDV) Persistent infection RNA-Seq Differentially expressed gene(DEG)

Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain

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Science China(Life Sciences) 2009年第2期52卷 155-162页

作者： BAI XingWen1, LI PingHua1, CAO YiMei1, LI Dong1, LU ZengJun1, GUO JianHong1, SUN DeHui1, ZHENG HaiXue2, SUN Pu1, LIU XiangTao1, LUO JianXun1 & LIU ZaiXin1 1 Key Laboratory of Animal Virology of Ministry of Agriculture, National foot-and-mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2 Veterinary Research Institute, Guangdong Agricultural Academy of Sciences, Guangzhou 510640, China Key Laboratory of Animal Virology of Ministry of Agriculture National Foot-and-Mouth Disease Reference Laboratory of China State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences Lanzhou China Veterinary Research Institute Guangdong Agricultural Academy of Sciences Guangzhou China

Two full-length genomic cDNA clones, pTA/FMDV and pCA/FMDV, were constructed that contained three point-mutants [A174G and A308G (not present in pTA/FMDV); T1029G] in the genome compared with the wild type A/AKT/58 strain of foot-and-mouth disease virus. These two viruses were rescued by co-transfection of pCA/FMDV with pCT7RNAP, which can express T7 RNA polymerase in BHK-21 cell-lines, or by transfection of the in vitro transcribed RNA. Their biological properties were analyzed for their antigenicity, virulence in suckling-mice (LD50) and growth kinetics in BHK-21 cells. The in vivo rescued viruses showed high pathogenicity for 3-day-old unweaned mice (LD50=10?7.5). However, the in vitro transcribed RNA derived from pTA/FMDV had lower pathogenicity for suckling-mice (LD50=10?6), and the in vivo transcribed RNA recovered from pCA/FMDV co-transfected with pCT7RNAP showed no significant differences from the wild type virus. These data showed that recovery of the infectious foot-and-mouth disease virus directly from the use of in vivo techniques was better than from in vitro methods. Furthermore, the reverse genetic procedure technique was simplified to a faster one-step procedure based on co-transfection with pCT7RNAP. These results suggest that in vivo RNA tran- scripts may be more valuable for engineering recombinant foot-and-mouth disease virus than in vitro RNA transcripts, and may contribute to further understanding of the biological properties, such as replication, maturation and quasispecies, of the foot-and-mouth disease virus.

关键词： foot-and-mouth disease virus infectious cDNA clones in vivo transcription A/AKT/58 strain

CpG DNA enhances the im-mune responses elicited by the DNA vaccine against foot-and-mouth disease virus in guinea pigs

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Chinese Science Bulletin 2001年第16期46卷 1376-1379页

作者： LI Guangjin LI Yingjie YAN Weiyao XU Quanxin WU Yongqing XIE Yong YOU Yongjin ZHENG Zhaoxin State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 200433China Institute of Veterinary Science Shanghai Academy of Agricultural SciencesShanghai 201106China Department of Biology Hong Kong University of Science and TechnologyHong KongChina

CpG DNA is DNA sequence that has immune stimulatory effects. Several lines of investigation over the past few years indicate that CpG DNA plays an important role in the induction of immune responses to DNA vaccines. In this study, CpG DNA-containing synthetic oligodeoxynucleotide (CpG-ODN) was cloned into the eukaryotic expression plasmid encoding a fusion protein containing b- galactosidase from E. coli and immunogenic epitopes of foot- and-mouth disease virus (FMDV) type O, and the immune responses induced by the plasmid were assayed. The results showed that guinea pigs immunized with the recombinant plasmid containing CpG-ODN generated a higher level of FMDV-neutralizing antibody and a stronger T cell proliferative response and protection against viral challenge than those receiving the plasmid containing no CpG-ODN. Our study demonstrated that it is an effective route to enhance the efficacy of DNA vaccines by inserting exogenous CpG DNA into the plasmids, and the DNA vaccine developed here is a promising candidate to prevent FMDV infection.

关键词： CpG DNA DNA vaccine foot-and-mouth disease virus immnostimulatory sequence.

Effect of amino acid mutation at position 127 in 3A of a rabbitattenuated foot-and-mouth disease virus serotype Asia1 on viral replication and infection

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Virologica Sinica 2014年第5期29卷 291-298页

作者： Aiguo Xin Mingwang Zhu Qi Hu Haisheng Miao Zhenqi Peng Yuwen He Lin Gao Huachun Li Yunnan Tropical and Subtropical Animal Virus Disease Laboratory Yunnan Animal Science and Veterinary Institute Yunnan Provincial Research Center for Veterinary Biological Products

An amino acid mutation(R127→I) in the 3A non-structural protein of an FMDV serotype Asia1 rabbit-attenuated ZB strain was previously found after attenuation of the virus. To explore the effects of this mutation on viral replication and infection, the amino acid residue isoleucine(I) was changed to arginine(R) in the infectious cDNA clone of the rabbit-attenuated ZB strain by sitedirected mutagenesis, and the R127-mutated virus was rescued. BHK monolayer cells and suckling mice were inoculated with the R127-mutated virus to test its growth property and pathogenicity, respectively. The effects of the R127 mutation on viral replication and virulence were analyzed. The data showed that there was a slight difference in plaque morphology between the R127-mutated and wild-type viruses. The growth rate of the mutated virus was lower in BHK-21 cells and its virulence in suckling mice was also attenuated. This study indicates that the R127 mutation in 3A may play an important role in FMDV replication in vitro and in pathogenicity in suckling mice.

关键词： foot-and-mouth disease virus(FMDV) 3A protein mutation replication ability virulence

Review of Air Dispersion Modelling Approaches to Assess the Risk of Wind-Borne Spread of foot-and-mouth Disease Virus

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Journal of Environmental Protection 2012年第9期3卷 1260-1267页

作者： Kritana Prueksakorn Taehyeung Kim Soyoung Kim Hyeontae Kim Ki Youn Kim Wongeun Son Chatchawan Vongmahadlek College of Veterinary Medicine Jeju National University Jeju Korea Department of Bio-Industrial Machinery Engineering Gyeongsang National University (Institute of Agriculture and Life Sciences) Jinju Korea Department of Environmental Engineering Changwon National University Changwon Korea Department of Industrial Health Catholic University of Pusan Busan Korea School of Chemical Engineering King Mongkut’s Institute of Technology Ladkrabang Bangkok Thailand

foot-and-mouth disease virus (FMDV) is one of the most economically serious veterinary pathogens due to its negative effects on livestock and its highly infectious nature via a variety of transmission paths through oral and inhalation routes. Measures to enhance outbreak management can be designed according to analytical results predicted by mathematical models for wind-borne dispersion, an important path of virus transmission. Accurate atmospheric dispersion models are useful tools for properly determining risk management plans, while inaccurate models may conversely lead to accidental loss in two possible ways. Overly strict measures, e.g., slaughter for too wide an area, can cause severe economic difficulties, including irreversible loss of business operations for a number of farms. On the contrary, inestimable loss potentially caused by lax controls is a persistent threat. In this paper, available modelling procedures for forecasting the spread of FMDV, which have been used since the 1970s, each having its advantages and limitations, are reviewed for the purpose of ensuring suitable application in various conditions of any future emergency cases.

关键词： foot-and-mouth Disease Virus (FMDV) Atmospheric Dispersion Model Gaussian Lagrangian Viral Production Model

Adverse Effects of Inactivated foot-and-mouth Disease Vaccine—Possible Causes Analysis and Countermeasures

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World Journal of Vaccines 2018年第4期8卷 81-88页

作者： Keshan Zhang Bingzhou Lu Huanan Liu Junhao Zhao Haixue Zheng Xiangtao Liu State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Disease Reference Laboratory Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou China

foot-and-mouth disease (FMD) is an infectious and sometimes fatal viral disease that affects cloven-hoofed animals, and Chinese government adopts compulsory immunization measures for FMD. The adverse effects of FMD vaccine to pigs, cattle and goats have been reported increasingly frequent during the spring and autumn seasons when large numbers of farm livestock are vaccinated. The financial losses caused by vaccine adverse effects have been a serious concern for both farmers and primary prevention personnel. There are various causative factors reported to involve into adverse effect of FMD vaccine, including the inappropriate vaccine production, transportation and storage, livestock poor tolerance, and unqualified vaccinating manipulations. Symptomatic treatment and early drug prevention have a certain effect on the adverse effects. To analyze causes and propose countermeasures, in the current study possible reasons during the production and processing procedures of inactivated FMD vaccine were reviewed and corresponding countermeasures were recommended. The review may provide references for better use of vaccine to prevent FMD.

关键词： foot-and-mouth Disease Inactivated Vaccine Adverse Effects Causes Analysis and Countermeasures

foot-and-mouth Disease Outbreak at Bageshwori Gaushala,Chitwan,Nepal

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Journal of Agricultural Science and Technology(A) 2018年第6期8卷 406-411页

作者： Milan Kandel Sachin Regmi Bablu Thakur Ramchandra Acharya Krishna Kaphle Faculty of Animal Science Veterinary Science and FisheriesAgriculture and Forestry UniversityBharatpur Metropolitan-15ChitwanNepal Department of Theriogenology Institute of Agriculture and Animal SciencesTribhuvan UniversityKhairehani Municipality-1ChitwanNepal

Cow being the national animal of Nepal has special place in the *** institutions have since prehistoric time reared and cared for cattle herds and the tradition still *** Bageshwori Gaushala(cow shelter),initiated by the Hindu Rastraguru Yogi Narhaharinath in mid-1990s is one of the largest of such nucleus indigenous cattle herds in *** cattle conservation strategy of Nepal has recognized the importance of this nucleus herd,kept by grazing in the surrounding *** approaches of managing the herd health is in practice and vaccination was never *** outbreak did happen in past but overall the herd never suffered severe *** April 2018,an outbreak of foot-and-mouth disease(FMD)occurred at Bageshwori,Gaushala,Devghat,Bharatpur Metropolitan-1,Chitwan district of *** the bovine herd studied,80(53.33%)out of 150 affected animals presented characteristics FMD symptoms as vesicular lesions,drooling salivation,foot lameness,high fever and *** prompt supportive treatment,the condition *** complete recovery of infected herd was observed after 45 *** analysis showed the presence of Aphthovirus serotype O in the clinical *** present study attempts to provide fresh insights into the recent FMD outbreak,clinical signs,their effective treatment protocol and preventive measures that must be practiced following FMD outbreak.

关键词： Aphthovirus foot-and-mouth disease outbreak serotype O.

Selection of Highly Susceptible Cell Lines to foot and mouth Disease Virus Infection

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Open Journal of Veterinary Medicine 2013年第5期3卷 263-266页

作者： O. Zabal N. Fondevila Instituto de Virologia CICVyA INTA Dr. Nicolas Repetto y De los Reseros s/n Hurlingham Argentina

Different non-established cultures were examined to find those that showed high sensitivity to foot and mouth Disease Virus (FMDV). Ovine kidney cultures showed high sensitivity to types A, O and C, and were suitable to detect viral infection in samples of animal, as well as cell culture origin. The level of detection in this system was up to ten times higher than in BHK21 cells, which were commonly used for FMDV isolation and production. Viral production levels in ovine kidney cultures ranged from similar to twice as high as in BHK21. Ovine kidney cultures maintained these characteristics for at least 18 passages, allowing their use as an alternative system for FMDV diagnoses.

关键词： foot-and-mouth Disease Tissue Culture Virus Isolation

Diagnostic assays developed for the control of foot-andmouth disease in India

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World Journal of Virology 2015年第3期4卷 295-302页

作者： Gaurav Kumar Sharma Sonalika Mahajan Rakesh Matura Saravanan Subramaniam Rajeev Ranjan Jitendra Biswal Manoranjan Rout Jajati Keshari Mohapatra Bana Bihari Dash Aniket Sanyal Bramhadev Pattnaik Project Directorate on foot and mouth Disease Indian Council of Agricultural Research

foot-and-mouth disease(FMD) is a highly contagious and economically devastating disease of livestock, primarily affecting cattle, buffalo and pigs. FMD virus serotypes O, A and Asia1 are prevalent in India and systematic efforts are on to control and eventually eradicate the disease from the country. FMD epidemiology is complex due to factors like co-circulation, extinction, emergence and re-emergence of genotypes/lineages within the three serotypes, animal movement, diverse farm practices and large number of susceptible livestock in the country. Systematic vaccination, prompt diagnosis, strict biosecurity measures, and regular monitoring of vaccinal immunity and surveillance of virus circulation are indispensible features for the effective implementation of the control measures. Availability of suitable companion diagnostic tests is very important in this endeavour. In this review, the diagnostic assays developed and validated in India and their contribution in FMD control programme is presented.

关键词： foot-and-mouth disease Diagnosis Serosurveillance Sero-monitoring Multiplex polymerase chain reaction Real-time polymerase chain reaction Lineage differentiating polymerase chain reaction

O型口蹄疫病毒结构蛋白基因VP1的克隆与原核表达(英文)

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Agricultural Science & Technology 2008年第5期9卷 55-58,154页

作者：付薇陈磊熊毅潘琼王常伟陈进喜胡晓静刘棋广西大学动物科学技术学院广西南宁530005 广西动物疫病预防控制中心广西南宁530001

According to the complete genome of foot-and-mouth disease virus(FMDV)type O,a pair of special primers was designed to amplify VP1 *** VP1 gene was amplified by RT-PCR and subsequently inserted into the expression vector pGEX-6p-1 and induced by *** SDS-PAGE showed the expressed protein was 51 kD in molecular *** the product was purified by GSTrap FF *** product was detected through Western-blot that showed the protein has *** provided fundamental data and materials for further investigation on diagnosis method of FMDV.

关键词： foot-and-mouth disease virus Structural protein VP1 Cloning Expression