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Suppressing the Undulation of Solvent Scattering Light by Constant Magnetic Field

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Chinese Science Bulletin 1994年第17期39卷 1439-1443页

作者：张勇黄贤智许金钩陈国珍 Department of Chemistry & Institute of Analytical Chemistry of Xiamen University Xiamen 361005 PRC

Synchronous fluorometry can cause a band-narrowing effect to improving the fluorometric selectivity (Fig. 1). However, since the △λ value selected is usually small (generally about 0—5 nm) in synchronous scanning, the interference of solvents scattering light (mainly Rayleigh scattering light) is so serious that the sensitivity and the detection limit are harmed greatly.

关键词： constant magnetic field effects (MFEs) resonant synchronous fluorometry solvent scattering light.

Investigation on labelling stomach cancer and K562 cell with fluorescence markers

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Chinese Science Bulletin 1995年第2期40卷 150-153页

作者：李建中章竹君邹克渭齐浩 Department of Chemistry Nanjing University Nanjing 210008 China Department of Chemistry Shaanxi Normal University Xi'an 710062 China Department of Biology Shaanxi Normal University Xi'an 710062 China

It is very important to quantitate the cytotoxicity of natural killer cell （NK cell） in the fields of cell immune assessment and life science studies. The currently used methods are based on labelling target with ra... 详细信息

关键词： time resolved fluorometry fluorescent marker target cell.

Microalgae Cultivation Using Offshore Membrane Enclosures for Growing Algae (OMEGA)

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Journal of Sustainable Bioenergy Systems 2013年第1期3卷 18-32页

作者： Patrick Wiley Linden Harris Sigrid Reinsch Sasha Tozzi Tsegereda Embaye Kit Clark Brandi McKuin Zbigniew Kolber Russel Adams Hiromi Kagawa Tra-My Justine Richardson John Malinowski Colin Beal Matthew A. Claxton Emil Geiger Jon Rask J. Elliot Campbell Jonathan D. Trent Advanced Organic Methods Penryn USA Department of Chemistry University of California Santa Cruz USA Department of Mechanical Engineering University of Nevada Reno USA Department of Ocean Sciences University of California Santa Cruz USA Dynamac Corporation NASA Ames Research Center Moffett Field USA NASA Ames Research Center Moffett Field USA School of Engineering University of California Merced USA SETI Institute Mountain View USA Universities Space Research Association Columbia USA

OMEGA is a system for cultivating microalgae using wastewater contained in floating photobioreactors (PBRs) deployed in marine environments and thereby eliminating competition with agriculture for water, fertilizer, and land. The offshore placement in protected bays near coastal cities co-locates OMEGA with wastewater outfalls and sources of CO2-rich flue gas on shore. To evaluate the feasibility of OMEGA, microalgae were grown on secondary-treated wastewater supplemented with simulated flue gas (8.5% CO2 V/V) in a 110-liter prototype system tested using a seawater tank. The flow-through system consisted of tubular PBRs made of transparent linear low-density polyethylene, a gas exchange and harvesting column (GEHC), two pumps, and an instrumentation and control (I&C) system. The PBRs contained regularly spaced swirl vanes to create helical flow and mixing for the circulating culture. About 5% of the culture volume was continuously diverted through the GEHC to manage dissolved oxygen concentrations, provide supplemental CO2, harvest microalgae from a settling chamber, and add fresh wastewater to replenish nutrients. The I&C system controlled CO2 injection and recorded dissolved oxygen levels, totalized CO2 flow, temperature, circulation rates, photosynthetic active radiation (PAR), and the photosynthetic efficiency as determined by fast repetition rate fluorometry. In two experimental trials, totaling 23 days in April and May 2012, microalgae productivity averaged 14.1 ± 1.3 grams of dry biomass per square meter of PBR surface area per day (n = 16), supplemental CO2 was converted to biomass with >50% efficiency, and >90% of the ammonia-nitrogen was recovered from secondary effluent. If OMEGA can be optimized for energy efficiency and scaled up economically, it has the potential to contribute significantly to biofuels production and wastewater treatment.

关键词： Biofuels Wastewater Treatment Microalgae Photobioreactor CO2 Mass Transfer Fast Repetition Rate fluorometry Instrumentation and Control

Front-Face Fluorescence Using UV-LED Coupled to USB Spectrometer to Discriminate between Virgin Olive Oil from Two Cultivars

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Food and Nutrition Sciences 2019年第2期10卷 119-127页

作者： Gilbert Yvon Mbesse Kongbonga Kaouther Ben Hassine Hassen Ghalila Dhafer Malouche Faculty of Science University of Bangui Bangui Central African Republic Laboratoire de Spectroscopie Atomique Moléculaire et Applications (LSAMA) Faculty of Sciences University of Tunis El Manar Tunis Tunisia Centre for Atomic Molecular Physics and Quantum Optics (CEPAMOQ) Faculty of Science University of Douala Douala Cameroon Laboratory of Biochemistry UR: Human Nutrition and Metabolic Disorder Faculty of Medicine Monastir Tunisia Institut de l’Olivier Unité Technologie et Qualité Sfax Tunisia National Institute for Applied Sciences and Technologies Tunis Tunisia Higher School of Agriculture of Mograne Mograne Tunisia Engineering School of Statistics and Information Analysis University of Carthage Tunis Tunisia

A simple setup using a 365 nm LED coupled to a USB spectrometer through an optical fibre, in a front-face fluorescence configuration, was used to investigate the ability of fluorescence spectroscopy technique to discriminate between varieties of olive oil. To achieve this task, Virgin Olive Oils (VOO) from two major Tunisian olive cultivars known as Chetoui and Chemlali were used. Spectral analysis showed a clear separation between these two VOO varieties. A one-way ANOVA attests that this discrimination is significant. The Principal Components Analyses (PCA) showed that the normalized fluorescence intensities are the good parameters for this discrimination. This observation strengthens the potential of our spectral parameters to perform reliable analysis.

关键词： Chemometrics fluorometry Oils Olive Oil PCA UV/Visible

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

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Journal of Water Resource and Protection 2018年第8期10卷 740-761页

作者： Nancy J. Leland James F. Haney Lim-Tex North Andover MA USA Department of Zoology University of New Hampshire Durham NH USA

The management of cyanobacteria and potential exposure to associated biotoxins requires the allocation of scarce resources across a range of freshwater resources within various jurisdictions. Cost effective and reliable methods for sample processing and analysis form the foundation of the protocol yielding reliable data from which to derive important decisions. In this study the utilization of new methods to collect, process and analyze samples enhanced our ability to evaluate cyanobacterial populations. Extraction of phycocyanin using the single freeze thaw method provided more accurate and precise measurements (CV 4.7% and 6.4%), offering a simple and cost-effective means to overcome the influence of morphological variability. In-vacuo concentration of samples prior to ELISA analysis provided a detection limit of 0.001 μg·L?1 MC. Fractionation of samples (?1) = ?0.279 + (1.368 ? Log PC (μg·L?1) while in an Aphanizomemon spp. dominant system Log MC (ng·L?1) = 0.385 + (0.449 ? Log PC (μg·L?1). These methods and sampling protocol could be used in other aquatic systems across a broader regional landscape to estimate the levels of microcystins.

关键词： Cyanobacteria Size Fractions fluorometry Monitoring Phycocyanin Extraction

Chlorophyll-a Fluorescence in Ecology： Theoretical Considerations and Examples around Marine Macroalgae

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Journal of Environmental Science and Engineering(A) 2013年第10期2卷 606-616页

作者： Vinicius Peruzzi de Oliveira Nuno Tavares Martins Yocie Yoneshigue-Valentin Alex Enrich-Prast Biogeochemistry Laboratory Department of Ecology Biology Institute Federal University of Rio de Janeiro Rio de Janeiro 21941-902. Brazil Macroalgae Laboratory Department of Botany Biology Institute Federal University of Rio de Janeiro Rio de Janeiro 21941-902 Brazil

Over the last decades, the usage of PAM （pulse amplitude modulated） fluorometers for assessment of chlorophyll-a fluorescence variations became widely applied on marine macroalgae physiology and ecophysiology researches. Due to the increased use of these methods, a large number of studies, mainly relating to macroalgae ecology and physiology were worldwide reported. In this context, it was also created a mismatch of concepts about fluorescence of the chlorophyll-a and its application. Under this background, this study compile and summarize the state of the art knowledge regarding to the chlorophyll fluorescence, contextualizing the use of the PAM method with the main factors regulating photosynthesis （light, temperature, salinity nitrogen and phosphorus） in marine macroalgae. Moreover, this study also references the most used terms and shows some examples found in literature about the applicability of fluorescence parameters. The herein findings and the discussed examples, helps to emphasize the importance of fluorescence usage, that highlights the understanding of photosynthetic responses in macroalgal physiology and ecology.

关键词： fluorometry PAM quenching analysis stress.

A high throughput sequential injection fluorometric procedure for the determination of protein in human serum

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A high throughput sequential injection fluorometric procedur...