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Human umbilical cord blood-derived mesenchymal stem cells promote regeneration of crush-injured rat sciatic nerves

Neural Regeneration Research 2012年第26期7卷 2018-2027页

作者： Mi-Ae Sung Hun Jong Jung Jung-Woo Lee Jin-Yong Lee Kang-Mi Pang Sang Bae Yoo Mohammad S. Alrashdan Soung-Min Kim Jeong Won Jahng Jong-Ho Lee Department of Craniofacial Structure & Functional Biology School of Dentistry Seoul National University Seoul Republic of Korea Department of Occupation and Environment Postgraduate College of Medicine Konkuk University Choong-ju Republic of Korea Department of Oral & Maxillofacial Surgery School of Dentistry Seoul National University Seoul Republic of Korea Department of Dentistry Oral and Maxillofacial Surgery Ajou University School of Medicine Suwon Republic of Korea Dental Research Institute Seoul National University Seoul Republic of Korea

Several studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells （1 ~ 106） or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchvmal stem cells promote the functinnal r~.RcJv^rv nf P.n I^h-inillr^4 ~r^i~tit, n^r~e

关键词： human umbilical cord blood-derived mesenchymal stem cells sciatic nerve crush injury fluorogold stem cells peripheral nerve regeneration regeneration neural regeneration

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β-Ⅲ-Tubulin: a reliable marker for retinal ganglion cell labeling in experimental models of glaucoma

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International Journal of Ophthalmology(English edition) 2015年第4期8卷 643-652页

作者： Shan-Ming Jiang Li-Ping Zeng Ji-Hong Zeng Li Tang Xiao-Ming Chen Xin Wei Department of Ophthalmology West China Hospital Sichuan University

·AIM: To evaluate the reliability of β-III-Tubulin protein as a retinal ganglion cell(RGC) marker in the experimental glaucoma model.·METHODS: Glaucoma mouse models were established by injecting polystyrene microbeads into the anterior chamber of C57BL/6J mice, then their retinas were obtained 14 d and 28 d after the intraocular pressure(IOP)was elevated. Retinal flat mounts and sections were double-labeled by fluorogold(FG) and β-III-Tubulin antibody or single-labeled by β-III-Tubulin antibody,then RGCs were counted and compared respectively.· RESULTS: IOP of the injected eyes were elevated significantly and reached the peak at 22.8 ±0.7 mm Hg by day 14 after injection, then dropped to 11.3 ±0.7 mm Hg by day 28. RGC numbers counted by FG labeling and β-III- Tubulin antibody labeling were 64 807 ± 4930 and64 614 ±5054 respectively in the control group, with no significant difference. By day 14, RGCs in the experimental group decreased significantly compared to the control group, but there was no significant difference between the FG labeling counting and the β-III-Tubulin antibody labeling counting either in the experimental group or in the control group. The result was similar by day 28, with further RGC loss.·CONCLUSION: Our result suggested that the β-III-Tubulin protein was not affected by IOP elevation and can be used as a reliable marker for RGC in experimental models of glaucoma.

关键词： β-III-Tubulin glaucoma fluorogold retinal ganglion cell

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Age-dependent slowing of enteric axonal transport in insulin-resistant mice

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World Journal of Gastroenterology 2013年第4期19卷 482-491页

作者： Kathy J LePard Joseph Cellini Department of Physiology Chicago College of Osteopathic MedicineMidwestern UniversityDowners GroveIL 60515United States

AIM:To investigate retrograde tracer transport by gastric enteric neurons in insulin resistant mice with low or high glycosylated hemoglobin(Hb).METHODS:Under anesthesia,the retrograde tracer fluorogold was superficially injected into the fundus or antrum using a microsyringe in KK Cg-Ay/J mice prior to onset of type 2 diabetes mellitus(T2DM;4 wk of age),at onset of T2DM(8 wk of age),and after 8,16,or 24 wk of untreated T2DM and in age-matched KK/HIJ mice.Six days later,mice were sacrificed by CO 2 narcosis followed by pneumothorax.Stomachs were removed and fixed.Sections from fundus,corpus and antrum were excised and mounted on a glass slide.Tracer-labeled neurons were viewed using a microscope and manually counted.Data were expressed as the number of neurons in short and long descending and ascending pathways and in local fundus and antrum pathways,and the number of neurons in all regions labeled after injection of tracer into either the fundus or the antrum.RESULTS:By 8 wk of age,body weights of KKAy mice(n=12,34±1 g) were heavier than KK mice(n=17,29±1 g;F(4,120)=4.414,P=0.002] and glycosylated Hb was higher [KK:(n=7),4.97%±0.04%;KKAy:(n=6),6.57%±0.47%;F(1,26)=24.748,P<0.001].The number of tracer labeled enteric neurons was similar in KK and KKAy mice of all ages in the short descending pathway [F(1,57)=2.374,P=0.129],long descending pathway [F(1,57)=0.922,P=0.341],local fundus pathway [F(1,53)=2.464,P=0.122],local antrum pathway [F(1,57)=0.728,P=0.397],and short ascending pathway [F(1,53)=2.940,P=0.092].In the long ascending pathway,fewer tracer-labeled neurons were present in KKAy as compared to KK mice [KK:(n=34),302±17;KKAy:(n=29),230±15;F(1,53)=8.136,P=0.006].The number of tracer-labeled neurons was decreased in all mice by 16 wk as compared to 8 wk of age in the short descending pathway [8 wk:(n=15),305±26;16 wk:(n=13),210±30;F(4,57)=9.336,P<0.001],local antrum pathway [8 wk:(n=15),349±20;16 wk:(n=13),220±33;F(4,57)=8.920,P<0.001],short ascending pathway [8 wk:(n=14),392±15;16 wk:(n=14),257±33;F(4,53)=1

关键词： Autonomic neuropathy Retrograde transport KKAy mice fluorogold Type 2 diabetes mellitus

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Evidence for the Projections from the Basal Nucleus of the Amygdala to the Primary Visual Cortex in the Cat

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生物物理学报 2009年第S1期25卷 250-250页

作者： Yuanxin Chen, Bin Zhu and Tiande Shou School of Life Sciences, Fudan University, Shanghai, P.R.China

The amygdaloid complex receives information from all sensory systems, especially from vision. Some researches (Amaral DG, 2003) show that in the primate brain, the amygdala

关键词： Amygdaloid complex Basal nucleus Area 17 fluorogold Evoked Potentials(EPs)

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Fluoro-Ruby和Fluoro-Gold示踪染色背根神经元在大鼠坐骨神经慢性卡压模型中的运用

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海南医学 2018年第23期29卷 3257-3259页

作者：黎琴文梁杰王冬梅陈波三峡大学人民医院宜昌市第一人民医院骨科湖北宜昌443000

目的将Fluoro-Ruby和Fluoro-Gold运用于大鼠坐骨神经慢性卡压模型,并探讨大鼠坐骨神经慢性卡压后Fluoro-Ruby染色的背根神经元是否能将轴突受损的背根神经元从众多背根神经元中分辨出来。方法将16只普通级SD雄性大鼠随机分成两组,每组8... 详细信息

目的将Fluoro-Ruby和Fluoro-Gold运用于大鼠坐骨神经慢性卡压模型,并探讨大鼠坐骨神经慢性卡压后Fluoro-Ruby染色的背根神经元是否能将轴突受损的背根神经元从众多背根神经元中分辨出来。方法将16只普通级SD雄性大鼠随机分成两组,每组8只,A组按照Mackinnon设计的方法建立坐骨神经慢性卡压模型,B组则仅暴露坐骨神经;术后2周,将5%Fluoro-Ruby和5%Fluoro-Gold各5μL分别注射卡压段神经的近端和远端。1周后,分别取A、B两组大鼠右侧L4、L5、L6背根神经节,行冰冻切片后立即在荧光显微镜下观察Fluoro-Ruby和Fluoro-Gold染色的背根神经元并照相保存。结果 Fluoro-Ruby和Fluoro-Gold可以分别对不同的背根神经元进行染色,B组大鼠背根神经节内可见(18.2±1.5)个Fluoro-Ruby示踪染色的背根神经元,而A组大鼠背根神经节内可见(38.1±4.5)个Fluoro-Ruby示踪染色的背根神经元细胞,数目较B组多,差异有统计学意义(P0.05)。结论 Fluoro-Ruby和Fluoro-Gold示踪染色背根神经节运用于大鼠坐骨神经慢性卡压后,Fluoro-Ruby染色的背根神经元可以将轴突受损的背根神经元从众多背根神经元中辨别出来。

关键词： Fluororuby fluorogold 背根神经节坐骨神经慢性卡压

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Evidence for the Projections from the Basal Nucleus of the Amygdala to the Primary Visual Cortex in the Cat

Evidence for the Projections from the Basal Nucleus of the A...

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第十一次中国生物物理学术大会暨第九届全国会员代表大会

作者： Yuanxin Chen,Bin Zhu and Tiande Shou School of Life Sciences,Fudan University,Shanghai,P.R.China

＜正＞The amygdaloid complex receives information from all sensory systems,especially from vision.Some researches（Amaral DG,2003）show that in the primate brain,the amygdala is

关键词： Amygdaloid complex Basal nucleus Area 17 fluorogold Evoked Potentials （EPs）

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