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Experimental Study on the Enhancement of Murine Splenic Lymphocyte Proliferation by Lycium Barbarum Glycopeptide

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2004年第5期24卷 518-520,527页

作者：杜光刘璐方建国 Department of Pharmacy Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

In order to investigate the immunoactivity of Lycium Barbarum glycopeptide (LBG), the routinely prepared murine splenic lymphocyte suspension was separately added into the samples with different concentrations (500, 100, 10, 1 μg/ml) of LBG as LBG groups. Blank control group in the absence of Lycium Barbarum glycopeptide or ConA and positive control group in the presence of 0.5 ml ConA but in the absence of LBG were created. 0.5 ml LBG samples with different concentrations in combination with 0.5 ml ConA (10 μg/ml) into each well to observe the synergic effects of LBG and ConA as LBG+ConA groups. After incubation for 72 h at 37 ℃, the samples were analyzed by CFSE-labeled cells combined with flow cytometry, and MTT. flow cytometry revealed that both LBG could enhance the murine splenic lymphocyte proliferative reaction. Combined use of LBG and ConA had synergic effects. MTT demonstrated that sample A could obviously promote the murine splenic lymphocyte proliferative reaction as compared with control group (P<0.01), while sample B could also enhance the lymphocyte proliferation at a high dose. In combination with ConA, sample A had synergic effects at high dose, while sample B showed obviously synergic effects (P<0.05). It was concluded that both samples (A and B) had strong immunocompetence.

关键词： Lycium Barbarum glycopeptide flow cytometer CFSE MTT immunocompetence

Inhibitory effect of resveratrol on the proliferation of GH3 pituitary-adenoma cells and voltage-dependent potassium current

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Neural Regeneration Research 2006年第6期1卷 481-484页

作者： Ming Chu Lanlan Wei Chao Wang Yu Cheng Kongbin Yang Baofeng Yang Department of Neurosurgery First Clinical Hospital Harbin Medical University Harbin 150001 Heilongjiang Province China Department of Microbiology Harbin Medical University Harbin 150001 Heilongjiang Province China Department of Pharmacology Harbin Medical University Harbin 150001 Heilongjiang Province Chnia

BACKGROUND ： Recent researches indicate that activation of potassium channel is likely to cause many kinds of cells to proliferate and differentiate; using chemical to block the potassium channel can restrain the proliferation of small lung-cancer cells, breast cancer, prostate cancer and human lymphocyte, etc. Previous researches proved that resveratrol （RE）, a selective estrogen receptor modulator （SERM）, could inhibit growth of GH3 cells, induce apoptosis, and resist tumor through interfering K^＋ channel. OBJECTIVE ： To investigate the effects of RE on voltage-dependent K^＋ current GH3 pituitary-tumor cells, DESIGN： Observational contrast study SETTING ： Department of Neurosurgery, the First Clinical Hospital of Harbin Medical University; Department of Microbiology, Harbin Medical University; Department of Pharmacology, Harbin Medical University. MATERIALS： GH3 pituitary-tumor cell line of rats was purchased from the American Type Culture Collection （ATCC）. RE and [3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl-tetrazolium bromide] （MTT） were obtained from Sigma Chemical Co, St Louis, USA; Ham＇s F-10 medium from Gibco BRL; Equine serum and fetal bovine serum from Hyclone Laboratones, Logan, UT; FACSCalibur flow cytometer from BD Company, USA, RE was dissolved in ethanol and stored at -20 ℃, It was diluted to different concentrations （10, 50, 100 μmol/L） with medium and extra cellular solution when needed, The final concentration of ethanol was less than 0.01%.METHODS： The experiment was carded out in the Department of Microbiology and Pharmacology of Harbin Medical University from March 2005 to January 2006, （1） Cell preparation： Proliferating indexes affected by 10, 50 and 100 μmol/L RE were measured with MTT, respectively. 0.000 1 volume fraction of ethanol was added into control group. Inhibitory rate of cellular growth was calculated as the following formula： Inhibitory rate （%） = （1 - A value in experimental group/A value in control group）×100%, The exp

关键词： flow cytometry cell cycle cell proliferation potassium channel volume fraction analysis of variance growth suppression Culture Collection potassium current estrogen receptor flow cytometer patch clamp cell line

Microchip imaging cytometer:making healthcare available,accessible,and affordable

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Opto-Electronic Advances 2022年第11期5卷 1-15页

作者： Xilong Yuan Todd Darcie Ziyin Wei J Stewart Aitchison Department of Electrical and Computer Engineering University of TorontoTorontoONM5S 3G4Canada

The Microchip Imaging cytometer(MIC)is a class of integrated point-of-care detection systems based on the combination of optical microscopy and flow *** devices have the attributes of portability,cost-effectiveness,and adaptability while providing quantitative measurements to meet the needs of laboratory testing in a variety of healthcare *** on the use of microfluidic chips,MIC requires less sample and can complete sample preparation ***,they can provide quantitative testing results simply using a finger prick *** decreased reagent consumption and reduced form factor also help improve the accessibility and affordability of healthcare services in remote and resource-limited *** this article,we review recent developments of the Microchip Imaging cytometer from the following aspects:clinical applications,microfluidic chip integration,imaging optics,and image ***,we provide an outlook of the field and remark on promising technologies that may enable significant progress in the near future.

关键词： microchip microfluidics flow cytometer imaging cytometer biosensors point-of-care testing biomedical engineering

The Role of Kappa and Lambda in Subclassification of B Cell Lymphoblastic Leukemia in Sudanese Patients Using flow Cytometry

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Open Journal of Blood Diseases 2016年第3期6卷 44-52页

作者： Hameeda Abd Eladeem Abdalla Amira Ahmed Khalid Humeida Eman Abbass Osama A. Altayeb Ghada M. Marghani Faculty of Medical Laboratory Sciences Al Neelain University Khartoum Sudan Faculty of Medicine Al Neelain University Khartoum Sudan flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis Khartoum Sudan Medical Laboratory Flow Cytometry Laboratory for Leukemia & Lymphoma Diagnosis Khartoum Sudan

Background: B-cell Acute lymphoblastic leukemia (B-ALL) is a neoplasm of lymphoblasts which are of B-cell lineage typically composed of small to medium sized blast cells, moderately condensed to dispersed chromatin with scanty cytoplasm and inconspicuous nucleoli, involving the bone marrow and/or blood. Methods and materials: This is a prospective cross-sectional study in which 50 blood and/or bone marrow samples of newly diagnosed patients (B-ALL) were tested for immunophenotyping. All samples were prepared for surface and cytoplasmic markers including kappa and lambda human antibody for 10 minutes in dark place and then run by the flow cytometer. Results: 64% of the study populations were males and 36% were females. Cases were classified according to immunophenotype and the age into different subtypes and showed the following frequencies: Pro B-ALL (8%), early pre B-ALL (56%), common B-ALL (16%), Pre-B-ALL (14%) and Mature B-ALL (only 6%). Surface immunoglobulin was positive in 10% and negative in 90% of all patients, showing 100% positivity in mature B-ALL and totally negative in other subtypes. While cytoplasmic immunoglobulin was positive in 16% and negative in 84% of all patients and was positive in 100% of Pre-B-ALL and in 50% of mature B-ALL. Surface kappa was more expressed in mature B-ALL than lambda giving a ratio of 2:1, while cytoplasmic kappa:lambda was 6:1 in Pre-B-ALL. Conclusion: Kappa and lambda have important role in B-ALL classification which necessitates their presence in immunophenotyping of B-ALL.

关键词： B Cell Acute Lymphoblastic Leukemia Immunoglobulin Kappa Lambda flow cytometer

Preliminary investigations on Arctic microalgae by jointapplication of fluorescent instruments

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Advances in Polar Science 2012年第2期23卷 128-132页

作者： ZHANG Fang HE Jianfeng GUANG Yingzhi Key Laboratory for Polar Science of State Oceanic Administration Polar Research Institute of ChinaShanghai 200136China

In vivo fluorescence has a wide application in analyzing microalgae, including assessing phytoplankton biomass, rates of primary production and physiological status. This study describes a preliminary investigation on the joint application of the three kinds of fluorescence analysis in the physiological study of microalgae. flow cytometry and fluorescence spectrometry were used to obtain the in vivo static fluorescence information of pigments, and a Pulsed-Amplitude-Modulation chlorophyll fluorometer was used to detect the dynamic fluorescence of chlorophyll. The validity of the joint application was proved by analyzing two labora- tory cultured Arctic microalgae, Pseudo-nitzschia delicatissima （Bacillariophyceae） and Thalassiosira sp. The higher value of minimum fluorescence yield in dark-adapted state （Fo）, actual photochemical efficiency of PSll （ФPSII）, and electron transport rate （ETR） exhibited positive results in a higher cell abundance and chlorophyll a content of P. delicatissima; whereas higher fl-carotene content of Thalassiosira sp. played an important role in the protection of photosynthesis.

关键词： Arctic microalgae flow cytometer fluorescence spectrum PAM chlorophyll fluorometer joint applica-tion

Increase of tumor necrosis factor receptor 1 expression in women with unexplained early spontaneous abortion

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Journal of Medical Colleges of PLA(China) 2004年第6期19卷 359-362,366页

作者：闫春芳于学文金辉李旭 Department of Gynecology & Obstetrics Fourth Hospital of Xi'an CityXi'an 710004China Center of Maternal and Child Health First Hospital of Xi'an Jiaotong UniversityXi'an 710061China Center of Molecular Biological Medicine First Hospital of Xi'an Jiaotong UniversityXi'an 710061China

Objective: To investigate membrane tumor necrosis factor receptor 1 protein expression level in decidua and concentration of soluble tumor necrosis factor receptor 1 in serum in women with unexplained early spontaneous abortion, threatened abortion, and compare the levels with healthy pregnant women. Methods: Thirty-seven women with unexplained early spontaneous abortion, 27 women with threatened abortion, and 34 healthy pregnant women undergoing artificial abortion of pregnancy at 6 - 10 weeks of gestation were selected. Decidual samples were collected when women were undergoing artificial abortion, and blood samples were collected at the same time. The level of membrane tumor necrosis factor receptor 1 in decidua was detected by flow cytometer, and the concentration of soluble tumor necrosis factor receptor 1 in sera was measured with an enzyme-linked immunosorbent assay. Results: The percentages of membrane tumor necrosis factor receptor 1 positive decidual cells were 16.42 ± 7.10 Mean ± SD for women with unexplained early spontaneous abortion and 13. 14 ± 6.30 for healthy pregnant women ( P < 0.05). Serum concentration of soluble tumor necrosis factor receptor 1 was significantly higher in women with unexplained early spontaneous abortion than in healthy pregnant women and in women with threatened abortion, and no difference was found between healthy pregnant women and women with threatened abortion. Conclusion: Women with unexplained early spontaneous abortion present significantly higher expression of tumor necrosis factor receptor 1 than healthy pregnant women, suggesting that over-expression of tumor necrosis factor receptor 1 may contribute to the development of early spontaneous abortion.

关键词： membrane tumor necrosis factor receptor 1 soluble tumor necrosis factor receptor 1 unexplained early spontaneous abortion unexplained recurrent spontaneous abortion flow cytometer