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Effect of chlorogenic acid on antioxidant activity of flos lonicerae extracts

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第9期8卷 673-679页

作者： WU Lan Department of Horticulture Beijing Vocational College of Agriculture Beijing 102442 China

flos lonicerae is a medically useful traditional Chinese medicine herb. However, little is known about the antioxidant properties of flos lonicerae extracts. Here the antioxidant capacity of water, methanolic and ethanolic extracts prepared from flos lonicerae to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reduce Fe3+ to Fe2+ is examined. Chlorogenic acid, a major component of flos lonicerae, is identified and further purified from 70% ethanolic extract with high performance liquid chromatography (HPLC) and its antioxidant capacity is characterized. The total phenolic compounds and chlorogenic acid con-tents in flos lonicerae are determined. The present results demonstrate that the flos lonicerae extracts exhibit antioxidant ac-tivity and chlorogenic acid is a major contributor to this activity.

关键词： flos lonicerae Chlorogenic acid Antioxidant

Chemical compositions and biological activities of flos lonicerae

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Asian Journal of Traditional Medicines 2023年第2期18卷 60-68页

作者： Yi’an Shi Luming Pan Luyan Yang Jing Sun Meng Zhang Yu Chen School of Life Science and Biopharmaceutics Shenyang Pharmaceutical UniversityShenyang 110016China

flos lonicerae is the dry flower bud of Lonicera japonica Thunb,and it is a very important Chinese herbal medicine in *** ancient times,it was widely used to treat the exopathogenic wind-heat,epidemic febrile diseases,sores,carbuncles and some infectious *** pharmacological research has indicated that it has anti-inflammatory,anti-bacterial,anti-viral and anti-oxidation *** paper reviews the chemical compositions and biological activities of flos lonicerae in order to provide reference for its application in clinical practice in the future.

关键词： flos lonicerae chemical composition biological activity

Separation and Purification of Macranthoidin B and Dipsacoside B from flos lonicerae by HP-20 and HP-SS Macroporous Resin

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Agricultural Science & Technology 2016年第4期17卷 765-768页

作者：卢凤来魏欢王磊颜小捷陈月圆李典鹏广西植物研究所广西植物功能物质研究与利用重点实验室广西桂林541006 桂林理工大学广西桂林541006

[Objective] This study was conducted to develop a method for rapidly separating macranthoidin B and dipsacoside B from flos lonicerae. [Method] HP-20 and HP-SS macroporous resin were applied to separate and purify macranthoidin B and dipsacoside B from flos lonicerae. The extract of flos lonicerae was first loaded onto an HP-20 column to enrich saponins, which were then separated by an HP-SS macroporous resin column to get pure macranthoidin B and dipsacoside B.[Result] The optimal HP-20 purification conditions included： a concentration of sample liquid at 4.8 mg/ml, a sample volume of 2 BV, an adsorption flow rate at 1.5BV/h, an ethanol concentration for desorption at 60%, a desorption volume of 3 BV,and a desorption flow rate at 1.5 BV/h. Total saponins were then separated by an HP-SS macroporous resin column which was eluted sequentially by water, 20%ethanol, 30% ethanol, 40% ethanol and 50% ethanol. Two purified compounds were obtained in fractions eluted by 40% ethanol and 50% ethanol, respectively. The two compounds were identified as macranthoidin B and dipsacoside B by13 C and1H nuclear magnetic resonance spectroscopy. [Conclusion] The combination of HP-20 and HP-SS macroporous resin could efficiently separate macranthoidin B and dipsacoside B from flos lonicerae.

关键词： flos lonicerae Macranthoidin B Dipsacoside B HP-20 macroporous resin HP-SS macroporous resin

Short-term Prediction of Occurrence Period of Main Diseases and Pests of flos lonicerae using Phenoecology

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Agricultural Science & Technology 2011年第6期12卷 858-861页

作者：苏桂花谢恩倍欧善生黄雪兰石化玉黄奉茂简峰黄艳花覃连红陈彩贤广西农业职业技术学院广西南宁530007 广西忻城县科学技术情报研究所广西来宾546200

The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson *** *** damage on flos lonicerae were investigated in *** planting area in XinCheng county of Guangxi Province during 2008-2010,which were coincided with the occurrence periods of related phenology of local Prunus persica *** *** Rootstock as indicator plant,the occurrence periods of three species of pests and one species of disease were predicted,respectively,and the method was simple and accurate,which could be the foundation for preventing these pests and diseases in the local field.

关键词： flos lonicerae Diseases and pests Phenological forecasting method

不同品种的金银花及金银花的不同部位中绿原酸含量的比较研究

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分析测试学报 2006年第z1期25卷 122-123,126页

作者：杨敏丽郝凤霞张林宁夏大学能源化工重点实验室宁夏银川750021 宁夏大学能源化工重点实验室宁夏银川750021 宁夏大学能源化工重点实验室宁夏银川750021

　　金银花是一种常用的中药材,其中绿原酸是其重要的有效成分,并被作为金银花药材及其制剂的质量控制指标之一[1-2].但不同品种的金银花其绿原酸含量都不相同,而且金银花不同部位绿原酸含量也不相同,为了更好地开发和利用金银花资源,... 详细信息

　　金银花是一种常用的中药材,其中绿原酸是其重要的有效成分,并被作为金银花药材及其制剂的质量控制指标之一[1-2].但不同品种的金银花其绿原酸含量都不相同,而且金银花不同部位绿原酸含量也不相同,为了更好地开发和利用金银花资源,本文以宁夏种植的5种不同品质的金银花为研究对象,采用超声提取HPLC检测的方法对不同品种的金银花及金银花不同部位中绿原酸的含量进行了比较研究,为金银花资源的合理利用提供理论依据.……

关键词：品种金银花药材同部位绿原酸含量比较研究 flos lonicerae 质量控制指标开发和利用资源有效成分研究对象合理利用超声提取中药材种植制剂品质宁夏理论检测

Leaf epidermal characters of Lonicera japonica and Lonicera confuse and their ecology adaptation

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Journal of Forestry Research 2007年第2期18卷 103-108页

作者：李强余龙江邓艳李为栗茂腾曹建华华中科技大学生命科学与技术学院湖北武汉430074 国土资源部岩溶动力学重点实验室中国地质科学院岩溶地质研究所广西桂林541004

The leaf epidermis of Japanese honeysuckle （Lonicera japonica Thunb.） and Wild Honeysuckle （Lonicera confusa） in the genus of flos lonicerae were mainly observed by scanning electron microscopes （SEM） to study the characteristics of stomata, trichomes and dermal cell, etc.. The results showed that stoma exists only on the lower epidermis and its distribution is irregular, and leaf epidermis consist of epidermis cells, stoma complexes and bushy trichomes including glandular hair and non-glandular hair. On the upper epidermis, anticlinal wall caves in sinuous groove to countercheck the transpiration. Evidences from leaf morphological structures serve as another proof on drought-resistant mechanisms. Some strumaes distributing regularly are hypothesized as oxalic calcium on the lower epidermis under laser scanning confocal microscopy （LSCM） with Fluo-3/AM, which can increase their endurance to drought stress. Therefore, the above characteristics of flos lonicerae can reduce the loss of water and make Japanese honeysuckle and Wild Honeysuckle adapt to the droughty environment at Karst area in southwest China. However, there is some difference of the two species. From the SEM （Scanning Electron Microscopy） result, it is shown that on the upper epidermis, some glandular hair regularly present along the midrib of Japanese honeysuckle, but Wild Honeysuckle has no glandular hair on the upper epidermis, which can verify the relationships of flos lonicerae species and provide the significance for classification of flos lonicerae.

关键词： Lonicera japonica Lonicera confuse flos lonicerae Dermal cell Stomata Trichomes Ecology adaptation