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Daisy fleabane

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流行歌曲（下半月） 2008年第3期 2-2页

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<i>Conyza sumatrensis</i>Intrapopulation Variation in Response to Glufosinate

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American Journal of Plant Sciences 2019年第9期10卷 1565-1582页

作者： Ivana Paula Ferraz Santos de Brito Caio Antonio Carbonari Bruna Barboza Marchesi Ana Karollyna Alves de Matos Leandro Tropaldi Nilda Roma-Burgos Edivaldo Domingues Velini Department of Crop Science Sao Paulo State University (UNESP) College of Agriculture Botucatu Sao Paulo Brazil Department of Crop Soil and Environmental Sciences University of Arkansas Fayetteville AR USA

We aimed to study the response of Conyza sumatrensis to different doses of glufosinate, intrapopulation variation in sensitivity to the herbicide, and the heritability of phenotypic response, and model the evolution of resistance. Three studies were conducted in the greenhouse with two repetitions. First, we tested doses of glufosinate (0, 50, 100, 200, 400, 800 g a.i. ha-1) plus a nontreated check, with four replications. Second, we examined the range in sensitivity of 44 plants to 200 g a.i. ha-1 glufosinate. Third, we evaluated the sensitivity of the progeny of six glufosinate-treated plants to 200 g a.i. ha-1 glufosinate. Plant response was evaluated visually and the ammonium content in leaf tissues was measured. Glufosinate at 400 g a.i. ha-1 caused the highest injury to *** plants. Ammonia accumulation occurred in response to glufosinate treatment, regardless of dose. Ammonia accumulation was correlated strongly with the level of visible plant injury;thus, it is a good indicator of herbicide efficacy. Sensitivity to glufosinate was highly variable within the population. Plants with high ammonia concentration (high injury) after treatment with glufosinate produced progenies that also had high ammonia concentrations after herbicide treatment. The variation in ammonia accumulation among siblings was high. Simulating the exclusion of plants that accumulated more ammonia produced a population that is expected to be less sensitive to glufosinate in the next generation. The stronger the selection pressures by a simulated treatment with glufosinate, the greater the reduction in ammonia accumulation in the progeny and expected sensitivity to glufosinate.

关键词： Ammonia Accumulation Differential Tolerance Glutamine Synthetase Herbicide Selection Pressure Sumatran fleabane

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Technique to Obtain Mitotic Chromosomes of <i>Conyza bonariensis</i>L. Cronquist (Asteraceae)

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American Journal of Plant Sciences 2015年第9期6卷 1466-1474页

作者： Juliana Maria de Paula Cecília Alzira Ferreira Pinto-Maglio Programa de Pós-Graduação em Genética Melhoramento Vegetal e Biotecnologia Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais Instituto Agron&ocircmico de Campinas Campinas Brazil Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais Instituto Agron&ocircmico de Campinas Campinas Brazil

Cytogenetic analysis requires cytological preparations that unequivocally reveal the chromosome number and permit optimal visualizations of chromosome morphology for the construction of karyotypes and ideograms. Chromosomal characterization is possible only by establishing these two parameters. To cytogenetically characterize the weed Conyza bonariensis (L.) Cronquist (Asteraceae), it was necessary to improve cytological analysis techniques to obtain optimal results. This species belongs to a genus whose plants have wide phenotypic plasticity. These plants can be morphologically differentiated by other types of analysis, and thus the application of this technique will serve as a reference for cytogenetical analysis of other groups of plants that have cytogenetic characteristics similar to those of C. bonariensis. The methodology described here highlights three main protocol steps: 1) root tip collection from newly germinated seed radicles and from young root tips of mature plants, 2) pretreatment of meristems with antimitotic agents, both isolated and combined, and 3) acid and enzymatic hydrolytic processes.

关键词： Hairy fleabane Cytogenetics Chromosomes Weed

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