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Journal of Integrative Agriculture 2023年第8期22卷 2335-2345页

作者： XIE Dong-wei LI Jing ZHANG Xiao-yu DAI Zhi-gang ZHOU Wen-zhi SU Jian-guang SUN Jian School of Life Science Nantong UniversityNantong 226019P.R.China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221P.R.China Jiangsu Sanshu Biotechnology Co. Ltd.Nantong 226001P.R.China

Anthocyanin is an important pigment that affects plant color and nutritional *** play an important role in plant anthocyanin synthesis and ***,the regulatory function of MYB transcription factors in anthocyanin synthesis in flax flowers is still *** this study,402 MYB transcription factors were identified in the flax *** MYB members are unevenly distributed on 15 *** R2R3-LuMYB members were divided into 32phylogenetic ***-PCR analysis showed that seven R2R3-LuMYB genes in the adjacent subfamily of the evolutionary tree had similar expression patterns,among which Lu MYB216 was highly expressed in the petals of different ***,gene editing of LuMYB216 in flax showed that the petal color,anther color and seed coat color of mutant plants were significantly lighter than those of wild-type plants,and the anthocyanin content of lumyb216 mutant plants was significantly *** analysis indicated that LuMYB216 was significantly positively correlated with the upstream regulator *** study systematically analyzed the MYB gene family in flax,laying a foundation for studying the regulation of LuMYB216 in flax flower anthocyanin synthesis.

关键词： flax MYB gene family anthocyanin biosynthesis LuMYB216 functional characterization

The NAC transcription factor LuNAC61 negatively regulates fiber development in flax (Linum usitatissimum L.)

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Journal of Integrative Agriculture 2024年第3期23卷 795-805页

作者： Dongwei Xie Jing Li Wan Li Lijun Sun Zhigang Dai Wenzhi Zhou Jianguang Su Jian Sun School of Life Sciences Nantong UniversityNantong 226019China Institute of Crop Cultivation and Tillage Heilongjiang Academy of Agricultural SciencesHarbin 150086China Institute of Bast Fiber Crops Chinese Academy of Agricultural SciencesChangsha 410221China Jiangsu Sanshu Biotechnology Co.Ltd. Nantong 226001China

flax is a crucial fiber crop that exhibits excellent textile properties and serves as a model plant for investigating phloem fiber development. The regulation of multiple genes significantly influences fiber development, notably involving NAC(NAM, ATAF1/2, CUC2) transcription factors in forming the fiber secondary cell wall(SCW).Overexpression of LuNAC61 in flax resulted in sparse top meristematic zone leaves and significantly reduced stem cellulose content. Scanning electron microscopy and staining observations revealed a significant reduction in fiber bundles. β-Glucuronidase(GUS) staining analysis demonstrated high activity of the LuNAC61 promoter in the bast fibers of the flax stem. Additionally, several members of the LuPLATZ and LuCesA families exhibited significant coexpression with LuNAC61. Subcellular localization indicated the presence of LuPLATZ24 protein in the nucleus and cytoplasm, LuNAC61 protein exclusively in the nucleus, and LuCesA10 in the nucleus and endoplasmic reticulum. LuPLATZ24 positively regulates LuNAC61, whereas LuNAC61 negatively affects LuCesA10, suggesting the involvement of a metabolic network in regulating flax fiber development. In conclusion, this study provides a critical opportunity for a comprehensive and in-depth analysis of the mechanisms governing flax fiber development and the potential use of biotechnology to enhance flax fiber yield.

关键词： flax fiber development Lu NAC61 gene function gene interaction

Anisotropic Visco-Elastoplastic Modeling of Quasi-Unidirectional flax Fiber Reinforced Epoxy Behavior:An Investigation on Low-Velocity Impact Response

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Journal of Renewable Materials 2018年第5期6卷 464-476页

作者： Marwa Abida Jamel Mars Florian Gehring Alexandre Vivet Fakhreddine Dammak Normandie Univ ENSICAENUNICAENCEACNRSCIMAP14000 CaenFrance Mechanical Modeling and Manufacturing Laboratory(LA2MP) National School of Engineers of SfaxUniversity of SfaxB.P 1173-3038SfaxTunisia Laboratory of Electromechanical Systems(LASEM) National Engineering School of SfaxUniversity of SfaxB.P.1173-3038SfaxTunisia

Based on experimental test results,flax fiber reinforced polymer composites are characterized by nonlinear visco-elastoplastic *** aim of this work is to model the quasi-unidirectional flax fiber reinforced composite behavior through a three dimensional formulation with orthotropic elasticity and orthotropic plasticity using Hill *** isotropic hardening and Johnson Cook parameters are identified from unidirectional tensile tests at different strain *** adjustment of Hill’s yield criterion is developed based on yield stresses obtained in tensile tests at different *** numerical integration of the constitutive equations is implemented in a user-defined material,UMAT subroutines for the commercial finite element code *** model parameters are identified using tensile tests,the model needs to be validated by confronting it with other experimental *** is why experimental and numerical three-point bending tests are carried out in order to validate the proposed model with tests that have not served for the ***,a numerical parametric study on low velocity impact of a flax/epoxy composite circular plate is investigated.

关键词： Visco-elastoplasticity anisotropic behavior impact simulation flax fiber composites

Accuracy of genomic selection in biparental populations of flax(Linum usitatissimum L.)

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The Crop Journal 2016年第4期4卷 290-303页

作者： Frank M.You Helen M.Booker Scott D.Duguid Gaofeng Jia Sylvie Cloutier Morden Research and Development Centre Agriculture and Agri-Food Canada Crop Development Centre Department of Plant Sciences University of Saskatchewan Ottawa Research and Development Centre Agriculture and Agri-Food Canada

flax is an important economic crop for seed oil and stem fiber. Phenotyping of traits such as seed yield, seed quality, stem fiber yield, and quality characteristics is expensive and time consuming. Genomic selection(GS) refers to a breeding approach aimed at selecting preferred individuals based on genomic estimated breeding values predicted by a statistical model based on the relationship between phenotypes and genome-wide genetic markers. We evaluated the prediction accuracy of GS(rMP) and the efficiency of GS relative to phenotypic selection(RE) for three GS models: ridge regression best linear unbiased prediction(RR-BLUP),Bayesian LASSO(BL), and Bayesian ridge regression(BRR), for seed yield, oil content, iodine value, linoleic, and linolenic acid content with a full and a common set of genome-wide simple sequence repeat markers in each of three biparental populations. The three GS models generated similar rMPand RE, while BRR displayed a higher coefficient of determination(R^2)of the fitted models than did RR-BLUP or BL. The mean rMPand RE varied for traits with different heritabilities and was affected by the genetic variation of the traits in the *** for seed yield generated a mean RE of 1.52 across populations and marker sets, a value significantly superior to that for direct phenotypic selection. Our empirical results provide the first validation of GS in flax and demonstrate that GS could increase genetic gain per unit time for linseed breeding. Further studies for selection of training populations and markers are warranted.

关键词： Genomic selection SSR flax Linseed Seed yield Fatty acid composition

Measurement and simulation of the suspension velocity of flax threshing material using CFD-DEM

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International Journal of Agricultural and Biological Engineering 2021年第5期14卷 230-237页

作者： Fei Dai Wenjuan Guo Xuefeng Song Ruijie Shi Jiangfei Qu Wuyun Zhao College of Mechanical and Electrical Engineering Gansu Agricultural UniversityLanzhou 730070China School of Cyber Security Gansu University of Political Science and LawLanzhou 730070China

In order to explore the aerodynamics characteristics of flax threshing material,and accurately obtain the movement trajectory of the material of each component in air-screen cleaning,the suspension velocities of flax grains,capsules,short stalks and capsule husks at different stages were measured by means of the suspension velocity *** experimental results showed that the suspension velocity range was 4.55-8.64 m/s for flax grains,6.46-10.90 m/s for flax capsules,3.23-7.75 m/s for flax short stalks,and 1.06-4.21 m/s for flax capsule *** on the experimental results,the overall interval distribution of each component of flax threshing material and the separation characteristics were discussed and the discrete element model of each component of the flax threshing material was *** simulation measurement of angle of repose of the material and the sliding friction angle with steel,the reliability of the established model was *** gas-solid coupling method was used to simulate the suspension velocity of each component of the flax threshing material,and the suspension velocity of the four components of flax threshing material was *** simulation measurement results were within the actual measurement range,showing that the test results were basically in line with the simulation *** simulation on the suspension velocity of different components of flax threshing material based on gas-solid coupling had high precision ***,the feasibility of the suspension velocity of the flax threshing material measured in the simulation test based on CFD-DEM coupling was verified.

关键词： flax threshing material suspension velocity CFD-DEM numerical simulation

Production of Pectinolytic Enzymes by Two Bacillus spp. Strains and Their Application in flax Degumming

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Transactions of Tianjin University 2019年第4期25卷 413-419页

作者： Jingping Ge Zhiyu Yang Renpeng Du Li Zhang Wenxiang Ping Dan Zhao Engineering Research Center of Agricultural Microbiology Technology Ministry of EducationHeilongjiang UniversityHarbin 150500China Key Laboratory of Microbiology School of Life SciencesHeilongjiang UniversityHarbin 150080China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300350China

This study demonstrated that Bacillus licheniformis HDYM-03 and Bacillus megaterium HDYM-09, isolated from a liquid sample of fl ax retting pool, were able to produce pectinolytic enzymes using polysaccharides as substrates. Bacillus megaterium HDYM-09 produced pectin lyase that exhibited the highest activity of 2116.71 ± 11.55 U/mL. Bacillus licheniformis HDYM-03 produced pectate lyase that exhibited the highest activity of 611.21 ± 14.54 U/mL. Based on these fi ndings, we constructed four retting systems to degrade the pectin substance. The results showed that the content of galacturonic acid in the mixed system was 529.21 μg/mL, the content of reducing sugar was 98.14 mg/mL, and the weight loss ratio of cells reached 19.49%, which were signifi cantly higher than those in other systems. The mixed system has more advantages, and the utilization rate of degumming was higher, which further ensured that the degumming can be carried out effi ciently and quickly. The mixed system exhibits feasible applications in the fi ber and textile industry.

关键词： flax Degumming Retting Pectin Pectate Pectinolytic enzyme Fiber

RESEARCH ON STRUCTURE AND PROPERTY OF OIL flax FIBER

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Journal of China Textile University(English Edition) 1989年第2期7卷 64-69页

作者：徐光华 Department of Textile Technology

In this paper investigations of the macromolecular chain structure, crystalline andmorphological structure of oil flax of San Xi and flax of East-North by means of such as elec-tron microscopy, X-ray diffraction, density and birefringence etc. is presented. The physical andmechanical properties of fibers are also measured. The results show that there is a little differencebetween the macrostructure of two kinds of flax fibers. The count which is an average linear den-sity of single fiber from different parts (root, middle, top of the stem), of the oil flax and that ofthe flax from East-North is 3.28 dtex and 3.29 dtex respectively. The modulus, breaking tenacityand elongation of oil flax are 1835.63 cN / tex, 61.08 cN / tex and 3.62% respectively. Com-paring this data with the data of flax, we find that there exists no appreciable difference.

关键词： electron microscopy X-ray diffraction birefringence crystallinity orientation degree breaking tenacity oil flax

Study on Dyeing Behavior of Modified flax

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Journal of Donghua University(English Edition) 2004年第5期21卷 46-50页

作者：郑来久李红刘剑宇 College of Textiles Donghua University Shanghai 200051 College of Textiles Dalian Institute of Light Industry Dalian Liaoning 116034professor College of Textiles Dalian Institute of Light Industry Dalian Liaoning 116034

The study of dyeability of the modified flax that uses dye-uptake to reflect its modifying effect is reported in this paper. The optimal technological condition is that the concentration of the modifying agent is 4 g/l, and NaOH is 6 g/l at liquor ratio of 1∶30 for 60 min. at 85℃.Dyeability include uptake rate and color fastness. Dye-uptake of the modified flax is twice more than that of the unmodified. And their color fastness doesnt fall and some of them raise 0.51 level.

关键词： flax cation modification dye-uptake dyeing behavior

Sodium nitroprusside as a signal molecule for up-regulating membrane characteristics,antioxidant defense system to improve flax productivity under water stress

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Oil Crop Science 2024年第3期9卷 160-169页

作者： N.M.Al-Ashkar B.A.Bakry H.M.S.El-Bassiouny M.M.S.Abdallah M.S.Sadak Botany Department Agricultural and Biological Research InstituteNational Research Centre33 El Bohouth Street P.O.12622DokkiGizaEgypt Field Crops Research Department Agricultural and Biological Research InstituteNational Research Centre33 El Bohouth Street P.O.12622DokkiGizaEgypt

Water stress is a critical environmental adversity that significantly impacts the growth,development,and yield of flax *** this study,flax seeds were cultivated under different water irrigation requirements(WIR)(100%,75%,and 50%)to investigate the effects of exogenously supplied nitric oxide(NO)donor sodium nitroprusside(SNP)as foliar treatments at concentrations of 0.0 mmol/L,0.5 mmol/L,1.0 mmol/L,and 2.0 mmol/*** stress led to a significant decrease in plant growth,photosynthetic pigments,yield components such as oil and total carbohydrate *** also resulted in an increase in leaf H2O2 production,lipid peroxidation levels and activities of enzymatic antioxidants including polyphenol oxidase,superoxide dismutase,and nitrate reductase ***,foliar application of SNP improved photosynthetic pigments and antioxidant defense system which mitigated the negative impact of water stress on growth and yield productivity by reducing oxidative damage caused by reactive oxygen species *** use of SNP also decreased H_(2)O_(2) accumulation levels,lipid peroxidation levels,and improved membrane *** treatment at concentration of 2 mmol/L showed superior results compared to other concentrations with extremely significant increases observed in yield characteristics such as oil content,total carbohydrate percentages,and unsaturated fatty acids to saturated fatty acids ratio.

关键词： flax Fatty acid Sodium nitroprusside Antioxidant enzymes Yield

Effectiveness of biofertilizers foliar application on yield and quality traits of flax(Linum usitatissimum L.)

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Oil Crop Science 2024年第2期9卷 91-101页

作者： Saied El Sayed A.B.Bakry O.A.Nofal M.A.Abo Horish Plant Nutrition Department National Research Centre33 El-Behouth St12622DokkiCairoEgypt Field Crops Department National Research Centre33 El-Behouth St12622DokkiCairoEgypt

flax is considered to be one of the most significant dual-purpose crops for oil and fiber production in Egypt and *** have a substantial impact on various metabolic processes,including increased photo-synthesis,endogenous hormone levels,ion absorption,nucleic acid synthesis,and protein *** factors collectively contribute to the growth and development of ***,this study aims to investigate how three biofertilizers(Algae extract,CMS as a by-product of yeast,and Metalosate multi minerals as amino acids)can enhance both the quantity and quality of flax seed yield under sandy soil *** field experiments were conducted at the Experimental Station of National Research Centre in Nubaria District,Behira Governorate,Egypt during two seasons(2021/2022)using a randomized complete block design(RCBD).The results revealed significant differences among all tested biofertilizers in terms of various characteristics studied in *** application of algae extract at a rate of 1.50 mL/L resulted in an increase in seed yield(ton/ha)by 26.69%&19.89%,straw yield(ton/ha)by 8.08%&17.12%,and oil yield(kg/ha)by 47.72%&33.69%compared to the control group during both seasons *** applications of algae extract at a rate of 1.50 mL/L along with CMS at a rate of 5 m L/L and amino acids at a rate of 1.50 mL/L demonstrated significantly higher macronutrient contents(N,P,K),micronutrient contents(Fe,Zn,Mn),seed oil content,and protein content in flax seeds during both *** highest values for seed oil content and protein content%were obtained through foliar application of amino acids at a rate of 1.50 mL/*** can be concluded that foliar sprays with these bio-fertilizers effectively improved flax performance by increasing seed straw and oil yields,nutrients oil,protein and fatty acids seeds contents.

关键词： flax Bio fertilizers Seeds yield Nutrient contents Quality