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GC-MS analysis,determination of total phenolics,flavonoid content and free radical scavenging activities of various crude extracts of Moringa peregrina(Forssk.) Fiori leaves

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Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 964-970页

作者： Mooza Al-Owaisi Nora Al-Hadiwi Shah Alam Khan Department of Pharmacy Oman Medical College

Objective:To perform phytochemical screening,estimate total phenolics,flavonoids and to evaluate antioxidant potential of Moringa peregrina(***) ***:The dried powdered leaves of ***(150 g) were extracted exhaustively by Soxhlet with ethanol and then fractionated into hexane,chloroform,ethy alacetate and *** the prepared extracts were also analyzed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the crude ***- Ciocalteu reagent and aluminium chloride colorimetric methods were used to estimate total phenolic and flavonoid content of *** peroxide and 1,1 diphenyl-2-picrylhydrazyl were used to determine in vitro antioxidant ***:Phytochemical analysis of ethanol extract showed presence of major classes of *** chromatography-mass spectrometry results revealed presence of 19 phytoconstituents in hexane extract,6 in ethyl acetate and 7 compounds in methanolic *** extract was found to contain the highest phenolic content and *** vitro antioxidant activities of all crude extracts were significant and comparable with the standard ascorbic ***:Results of this study show that the leaves of *** are the rich source of phenolic compounds that can play an important role in preventing the progression of many diseases.

关键词： Antioxidant activity flavonoid content GC-MS analysis Moringa peregrina Total phenolics

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Fruiting increases total content of flavonoids and antiproliferative effects of Cereus jamacaru D.C. cladodes in sarcoma 180 cells in vitro

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Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 66-72页

作者： Jean Carlos Vencioneck Dutra Juda Ben-Hur de Oliveira Vanessa Silva dos Santos Paula Roberta Costalonga Pereira Jean Moises Ferreira Maria do Carmo Pimentel Batitucci Laboratorio de Genetica Vegetal e Toxicologica Departamento de Ciencias BiologicasUniversidade Federal do Espirito Santo Laboratorio de Biologia Molecular e Expressao Genica Departamento de Ciências BiologicasUniversidade Federal de Alagoas

Objective: To evaluate the influence of fruiting phenological stage on total flavonoid content, antioxidant activity, and antiproliferative effects of Cereus jamacaru(C. jamacaru)(mandacaru) cladodes and fruit. Methods: Fruit and cladodes at vegetative and fruiting stage of C. jamacaru were collected. The fruit was dissected and bark, pulp, and seeds were separated. Vegetative and fruiting cladodes, together with bark, pulp, and seeds were used to obtain five hydroalcoholic extracts. The extracts were investigated for total flavonoid content, using AlCl3 colorimetric method, antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity and Fe^(2+) ion chelating activity, and in vitro antiproliferative effects(sarcoma 180 cells) by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay. Results: The extract of C. jamacaru cladodes at the fruiting stage showed higher flavonoid content compared to the other extracts. Seed extracts showed the highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assays, and the extract of cladodes at vegetative stage showed better antioxidant activity in Fe^(2+) ion chelating activity. The extract of fruiting cladodes promoted higher antiproliferative effects compared to the other extracts. Conclusions: These findings suggest that fruiting increases the content of flavonoids and antiproliferative effects of C. jamacaru cladodes. Data reinforce the potential use of C. jamacaru cladodes and fruits as natural antioxidants and potent anticancer agent.

关键词： Mandacaru flavonoid content DPPH ABTS Fe^(2+) ion chelating activity MTT assay Sarcoma 180 cells

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