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Pollen Typhae Total flavone Inhibits Endoplasmic Reticulum Stress-Induced Apoptosis in Human Aortic-Vascular Smooth Muscle Cells through Down-Regulating PERK-eIF2α-ATF4-CHOP Pathway

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Chinese Journal of Integrative Medicine 2019年第8期25卷 604-612页

作者： CHEN Ming-tai HUANG Ruo-lan OU Li-jun CHEN Ying-nan MEN Ling CHANG Xiao WANG Ling YANG Yu-zhu ZHANG Zhong Cardiovascular Department Shenzhen Traditional Chinese Medicine Hospital Guangzhou University of Traditional Chinese Medicine

Objective: To test the hypothesis that the inhibition of endoplasmic reticulum(ER) stress-induced apoptosis in oxidized low-density lipoproteins(ox-LDL)-induced human aortic-vascular smooth muscle cells(HA-VSMCs) was associated with suppression of the protein kinase RNA-like ER kinase(PERK)-eukaryotic translation initiation factor 2α(e IF2α)-activating transcription factor 4(ATF4)-CCAAT/enhancer binding protein homologous protein(CHOP) signaling pathway by Pollen Typhae total flavone(PTF). Methods: Primary HA-VSMCs were cultured and identified. The cultured HA-VSMCs were randomized into 5 groups, including a normal control group, an ox-LDL group(70 μg/m L high ox-LDL), an HPTF group(70 μg/m L high ox-LDL+500 μg/m L PTF), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), and a LPTF group(70 μg/m L high ox-LDL+100 μg/m L PTF) in the first part;and a normal control group, an ox-LDL group(70 μg/mL high ox-LDL), an MPTF group(70 μg/m L high ox-LDL+250 μg/m L PTF), a sh RNA group(transducted with PERK shRNA lentiviral particles), a scramble shRNA group(transducted with control shRNA lentiviral particles), an MPTF+ox-LDL+shRNA group(250 μg/mL PTF+70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) and an ox-LDL+shRNA group(70 μg/mL high ox-LDL+PERK shRNA lentiviral particles) in the second part. The protein expression levels of ER-associated apoptosis proteins were detected by Western blot, and their m RNA expression levels were detected by quantitative real-time reverse transcription-polymerase chain reaction. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was applied to test cel viability, and the level of apoptosis was monitored by flow cytometry. Results: The MTT assay and flow cytometry showed that the ox-LDL group had a significant increase in apoptosis, which was attenuated in PTF treatment groups and sh RNA groups. Moreover, the ox-LDL group had increased protein and m RNA levels of binding immunoglobulin protein and ER-associated

关键词： Pollen Typhae total flavone endoplasmic reticulum stress protein kinase RNA-like endoplasmic reticulum kinase-eukaryotic translation initiation factor 2a -activating transcription factor 4-CCAAT/enhancer binding protein homologous protein pathway apoptosis vulnerable atherosclerotic plaque

Mahuangchiside, a New flavone Glycoside from Chirita fimbrisepala

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Chinese Chemical Letters 2000年第2期11卷 131-132页

作者： Li Dong ZHOU Jing Guang YU Jia GUO (Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094) Chinese Acad Med Sci Inst Med Plant Dev Beijing 100094 Peoples R China Peking Union Med Coll Beijing 100094 Peoples R China

A new flavone glycoside, named mahuangchiside, was isolated from the root of Chirita fimbrisepala Hand.-Mazz. The structure was elucidated as 5,4'-dihydroxy-6-methoxyflavone-7-O-beta-D-xylopyranosyl(1-->2)-beta-D-xylo... 详细信息

关键词： Chirita fimbrisepala Gesneriaceae mahuangchiside flavone flavonoid

A New flavone from the Roots of Uvaria macrophylla

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Chinese Chemical Letters 2001年第9期12卷 791-792页

作者： Hai Ling ZHANG Ruo Yun CHEN Institute of Materia Medica Chinese Academy of Medicinal Sciences&Peking Union Medical CollegeBeijing 100050

A New flavone named Macrophyllol was isolated from the roots of Uvaria macrophylla. The structure of 1 was elucidated on the basis of spectroscopic evidence.

关键词： Uvaria macrophylla flavone Macrophyllol

Synthesis of 4'-Methoxy flavone

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Chinese Chemical Letters 2000年第7期11卷 565-566页

作者： Lei ZOU Xiao Juan YE Yuan Lian LIU Zhen Bai CAO(Department of Chemistry, Suzhou University, Suzhou 215006) Suzhou Univ Dept Chem Suzhou 215006 Peoples R China

Synthesis of 4'-methoxyflavone, first by the reaction of 4 - methoxy acetophenone enamine with heptanedioyl chloride, then by dehydrogenation, is described.

关键词： Enamine heptanedioyl chloride tetrahydroflavone flavone synthesis

A New Diels-Alder Type Adduct and a New flavone from the Stem and Root Bark of Morus mongolica

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Chinese Chemical Letters 2005年第11期16卷 1474-1476页

作者： Jie KANG Ruo Yun CHEN De Quan YU Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050

A new Diels-Alder type adduct mongolicin G （1） and a new flavone 5＇-（1＂, 1＂-dimethylallyl）-5,7,2＇,4＇-tetrahydroxyflavone （2） were isolated from the stem and root bark of Morus mongolica. Their structures we... 详细信息

关键词： Morus mongolica Diels-Alder type adduct mongolicin G flavone 5'-(1",1"-dimethylallyl)-5,7,2',4'-tetrahydroxyflavone.

A Minor New flavone from Scutellaria baicalensis Georgi

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Chinese Chemical Letters 2002年第5期13卷 428-429页

作者： HongYanWANG SuiXuXU 等 DepartmentofBiochemistry HongKongUniversityofScienceandTechnologyClearWaterBayHongKong DpeartmentofChemistry ShenyangPharmaceuticalUniversityShenyang110016

A new flavone, 6, 2-dihydroxy-5, 7, 8, 6-tetramethoxyflavone, was isolated from the roots of Scutellaria baicalensis. Its structure was established on the basis of spectral evidences.

关键词： flavone 6,2-dihydroxy-5,7,8,6-tetramethoxyflavone Scutellaria baicalensis Georgi.

Development and Validation of a HPLC Method for Determination of flavone Glycoside-Camellianin B in Cephalotaxus Sinensis

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Journal of Beijing Institute of Technology 2017年第1期26卷 129-134页

作者： Yongqian Zhang Zhihui Xu Chao Wei Yan Chen Yulin Deng School of Life Science Beijing Institute of Technology Beijing 100081 China

A simple,rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis（***）a natural plant with anti-hyperglycemic effect,was developed and validated by reversed phase liquid *** separation was achieved on a C18 column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min-1 with the acetonitrile-water containing0.1%formic acid（19∶81,v/v）.The detection wavelength was set at 330 *** calibration curve was linear over the concentration range of 0.25-50μg·mL-1 with correlation coefficients larger than0.999 *** limit of detection and limit of quantification were 0.09μg·mL-1and 0.25μg·mL-1,*** precisions and accuracy for all samples were *** validated method has been successfully applied for the quantification of camellianin B in *** harvested in different months and may also be used as the quality evaluation of this herb medicine.

关键词： column liquid chromatography flavone flavone glycoside

New flavone and isoflavone glycoside from Belamcanda chinensis

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Chinese Chemical Letters 2007年第2期18卷 158-160页

作者： Li Jin Hai Sheng Chen Zhao Bao Xiang Shuang Liang Yong Sheng Jin Jian Guo Liu College of Pharmacy Second Military Medical UniversityShanghai 200433China College of Bio-information Chongqing University of Posts and TelecommunicationsChongqing 400065China

The new flavone, 5,4′-dihydroxy-6,7-methylenedioxy-3′-methoxyflavone, and one new isoflavone glycoside, 3′,5′-dimethoxy irisolone-4′-O-β-d-glucoside were isolated from the rhizomes of Belamcanda chinensis. Their... 详细信息

关键词： Belamcanda chinensis flavone Isoflavone glycoside

Total flavone of Abelmoschus manihot suppresses epithelial-mesenchymal transition via interfering transforming growth factor-β1 signaling in Crohn's disease intestinal fibrosis

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World Journal of Gastroenterology 2018年第30期24卷 3414-3425页

作者： Bo-Lin Yang Ping Zhu You-Ran Li Min-Min Xu Hao Wang Li-Chao Qiao Hai-Xia Xu Hong-Jin Chen Department of Colorectal Surgery The Affiliated Hospital of Nanjing University of Chinese Medicine

AIM To explore the role and mechanism of total flavone of Abelmoschus manihot(TFA) on epithelial-mesenchymal transition(EMT) progress of Crohn's disease(CD) intestinal *** First,CCK-8 assay was performed to assess TFA on the viability of intestinal epithelial(IEC-6) cells and select the optimal concentrations of TFA for our further *** cell morphology,wound healing and transwell assays were performed to examine the effect of TFA on morphology,migration and invasion of IEC-6 cells treated with TGF-β*** addition,immunofluorescence,real-time PCR analysis(q RT-PCR) and western blotting assays were carried out to detect the impact of TFA on EMT ***,western blotting assay was performed to evaluate the function of TFA on the Smad and MAPK signaling ***,the role of co-treatment of TFA and si-Smad or MAPK inhibitors has been examined by q RTPCR,western blotting,morphology,wound healing andtranswell *** In this study,TFA promoted transforming growth factor-β1(TGF-β1)-induced(IEC-6) morphological change,migration and invasion,and increased the expression of epithelial markers and reduced the levels of mesenchymal markers,along with the inactivation of Smad and MAPK signaling ***,we revealed that si-Smad and MAPK inhibitors effectively attenuated TGF-β1-induced EMT in IEC-6 ***,co-treatment of TFA and si-Smad or MAPK inhibitors had better inhibitory effects on TGF-β1-induced EMT in IEC-6 cells than either one of *** These findings could provide new insight into the molecular mechanisms of TFA on TGF-β1-induced EMT in IEC-6 cells and TFA is expected to advance as a new therapy to treat CD intestinal fibrosis.

关键词： Crohn’s disease Intestinal fibrosis Epithelialto-mesenchymal transition Total flavone of Abelmoschus manihot Transforming growth factor-β1/Smad signaling Transforming growth factor-β1/non-Smad signaling