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Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction

Neural Regeneration Research 2013年第23期8卷 2190-2197页

作者： Jing Liu Xiaofeng Wang Ying Liu Na Yang Jing Xu Xiaotun Ren Department of Neonatology and NICU of Bayi Children's Hospital General Hospital of Beijing Military Command

From pregnancy to parturition, Sprague-Dawley rats were daily administered a low protein diet to establish a model of intrauterine growth restriction. From the 12th day of pregnancy, 300 mg/kg taurine was daily added to food until spontaneous delivery occurred. Brain tissues from normal neonatal rats at 6 hours after delivery, neonatal rats with intrauterine growth restriction, and neo- natal rats with intrauterine growth restriction undergoing taurine supplement were obtained for fur- ther experiments. The terminal deoxyribonucleotidyl transferase （TdT）-mediated biotin-16-dUTP nick-end labeling assay revealed that the number of apoptotic cells in the brain tissue of neonatal rats with intrauterine growth restriction significantly increased. Taurine supplement in pregnant rats reduced cell apoptosis in brain tissue from neonatal rats with intrauterine growth restriction. Immu- nohistochemical staining revealed that taurine supplement increased glial cell line-derived neuro- trophic factor expression and decreased caspase-3 expression in the cerebral cortex of intrauterine growth-restricted fetal rats. These results indicate that taurine supplement reduces cell apoptosis through the glial cell line-derived neurotrophic factor-caspase-3 signaling pathway, resulting in a protective effect on the intrauterine growth-restricted fetal rat brain.

关键词： neural regeneration intrauterine growth restriction fetal rats brain neural cells taurine cell apop-tosis glial cell line-derived neurotrophic factor caspase-3 neural development grants-supportedpaper neuroregeneration

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An improved primary culture method for hippocampal neurons in fetal rats and MAP2 identification

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TMR Integrative Medicine 2018年第1期2卷 1-6页

作者： Yi-Ran Shao Yong Chang DIWU Tao Yue First Clinical Medical College of Shaanxi University of Chinese Medicine Xianyang 712046 China

Objective： To establish a simple, effective and high-purity primary culture method for fetal rat hippocampalneurons. Methods： Wistar rats of gestational age 18 days were taken and the brain tissue was separated under themicroscope. Single neuronal cells were obtained by digestion with Brain Dissociation Kit, and then were seeded incell plates to observe the basic morphologic structure after 24h, 3d, and 5d. Immunofluorescence of microtubuleassociated protein 2 was applied to assess cell purity of the culture. Results： The hippocampal neurons obtained inthis culture method are in good condition and grow vigorously. On the 7th day after culture, the purity of neuronswas up to 99.62%. Conclusion： The method is simple and effective for obtaining the high-purity and stableneurons.

关键词： Hippocampal neurons fetal rats Primary culture

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