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Probiotic effect of ferulic acid esterase-producing Lactobacillus plantarum inoculated alfalfa silage on digestion,antioxidant,and immunity status of lactating dairy goats

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Animal Nutrition 2022年第4期11卷 38-47页

作者： Fuhou Li Baibing Zhang Yixin Zhang Xia Zhang Samaila Usman Zitong Ding Lizhuang Hao Xusheng Guo State Key Laboratory of Grassland Agro-ecosystems School of Life SciencesCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou730000China Probiotics and Biological Feed Research Centre Lanzhou UniversityLanzhou730000China Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province The Academy of Animal and Veterinary SciencesState Key Laboratory of Plateau Ecology and AgricultureQinghai UniversityXining810016China

A feeding experiment was conducted to determine the effects of inoculating alfalfa silage with a ferulic acid esterase-producing inoculum on feed digestibility,rumen fermentation,antioxidant,and immunity status of lactating dairy *** dairy goats were distributed into 2 experimental groups consisting of control diet(Lp MTD/1,including Lactobacillus plantarum MTD/1 inoculated silage)against diet containing silage treated with ferulic acid esterase-producing *** A1(Lp A1).Alfalfa silage inoculated with a ferulic acid esterase-producing Lp A1 had better fermentation quality than the Lp MTD/*** application of Lp A1 improved silage antioxidant capacity as indicated by greater total antioxidant capacity(T-AOC),superoxide dismutase(SOD)and glutathion peroxidase(GSH-Px)activities in Lp A1 treated silage versus Lp MTD/1 *** with Lp MTD/1 treated group,inoculation of silage with Lp A1 increased apparent total tract digestibility of dietary dry matter,organic matter and crude protein,and ruminal concentrations of total volatile fatty acids,acetate,propionate and isobutyrate as *** results of current study also demonstrated improved antioxidant capacity and immune performance of dairy goats with Lp A1 *** Lp A1-treated silage increased dairy goats’serum antioxidase activity,such as T-AOC,SOD,GSH-Px and catalase,and the serum concentration of immunoglobulin A,while decreased tumor necrosis factor a,interleukin(IL)-2 and *** addition,compared with Lp MTD/1,diet containing alfalfa silage inoculated with Lp A1 endowed dairy goats’milk with greater fat and protein contents,improved dairy goat milk quality without affecting feed efficiency.

关键词： ferulic acid esterase Silage Digestibility Antioxidant Immunity

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Effects of inoculating feruloyl esterase-producing Lactiplantibacillus plantarum A1 on ensiling characteristics,in vitro ruminal fermentation and microbiota of alfalfa silage

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1713-1729页

作者： Fuhou Li Samaila Usman Wenkang Huang Mengya Jia Zohreh Akhavan Kharazian Tao Ran Fadi Li Zitong Ding Xusheng Guo State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000People’s Republic of China Probiotics and Biological Feed Research Centre Lanzhou UniversityLanzhou 730000People’s Republic of China State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems School of Life SciencesLanzhou UniversityNo.222 South Tianshui RoadLanzhou 730000People’s Republic of China

Background ferulic acid esterase(FAE)-secreting Lactiplantibacillus plantarum A1(Lp A1)is a promising silage inoculant due to the FAE’s ability to alter the plant cell wall structure during ensiling,an action that is expected to improve forage ***,little is known regarding the impacts of Lp A1 on rumen *** research assessed the influences of Lp A1 in comparison to a widely adopted commercial inoculant Lp MTD/1 on alfalfa’s ensilage,in vitro rumen incubation and *** Samples of fresh and ensiled alfalfa treated with(either Lp A1 or Lp MTD/1)or without additives(as control;CON)and ensiled for 30,60 and 90 d were used for fermentation quality,in vitro digestibility and batch culture *** treated silage had lower(Pacid concentration and dry matter(DM)loss,but higher(P=0.001)lactic acid concentration than the CON during *** to the CON and Lp MTD/1,silage treated with Lp A1 had lower(Pferulic acid *** silage treated with Lp MTD/1,silage treated with Lp A1 had significantly(P<0.01)improved ruminal gas production and digestibility,which were equivalent to those of fresh *** PCR analysis indicated that Lp A1 inoculation improved the relative abundances of rumen’s total bacteria,fungi,Ruminococcus albus and Ruminococcus flavefaciens,while the relative abundance of methanogens was reduced by Lp MTD/1 compared with *** component analysis of rumen bacterial 16S rRNA gene amplicons showed a clear distinction between CON and inoculated treatments without noticeable distinction between Lp A1 and Lp MTD/1 *** analysis revealed differences in the relative abundance of some bacteria in different taxa between Lp A1 and Lp MTD/1 *** treated with Lp A1 exhibited improved rumen fermentation characteristics due to the inoculant effects on the rumen micr

关键词： Digestibility ferulic acid esterase In vitro fermentation Rumen microbial community Silage

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Breakdown of Corn Fiber by a Metagenomic Ferulolyl esterase in Combination with Glycosyl Hydrolases

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Advances in Enzyme Research 2021年第4期9卷 91-100页

作者： Dominic W. S. Wong Victor J. Chan Hans Liao Western Regional Research Center United States Department of Agriculture Agricultural Research Service Albany USA Cargill Biotechnology Development Center Minneapolis USA

A feruloyl esterase (FAE-C6) gene of 957 bp was isolated from rumen microbial metagenome, subcloned into pET32b vector, and expressed in Escherichia coli. The enzyme purified in active form, consisted of 319 amino acid residues, with a molecular weight of 43.7 based on SDS-PAGE. Homology modeling showed that the FAE contained the catalytic triad composed of Ser154-Asp263His295 and a classical Gly-X-Ser154-X-Gly nucleophile motif commonly found in esterases. The FAE-C6 was characterized using corn fiber as substrate. Its combining action with glycoside hydrolases (C, X, A) individually and in various combinations was studied with focus on the difference in the effects on FA and sugar release. Glycoside hydrolases with endo-xylanase included in the enzyme mixture showed significant impact on increasing the FA yield. For the release of sugar, FAE enhanced the yield in all hydrolase combinations moderately and endo-xylanase was not the key factor in the enzyme formulation.

关键词： Feruloyl esterase ferulic acid esterase ferulic acid Metagenomics Corn Fiber

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