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The transcription factor GATA10 regulates fertility conversion of a two-line hybrid tms5 mutant rice via the modulation of UbL40 expression^∞

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Journal of Integrative Plant Biology 2020年第7期62卷 1034-1056页

作者： Jing Jin Songtao Gui Qian Li Ying Wang Hongyuan Zhang Zhixuan Zhu Hao Chen Yueyang Sun Yu Zou Xingguo Huang Yi Ding State Key Laboratory of Hybrid Rice Department of GeneticsCollege of Life SciencesWuhan UniversityWuhan 430072China Institute of Vegetable Wuhan Academy of Agricultural SciencesWuhan 430072China Wuhan Wuda Tianyuau Bio-Tech Co. Ltd.Wuhan 430070China

The thermosensitive genic male sterile 5(tms5)mutation causes thermosensitive genic male sterility in rice(Oryza sativa)through loss of RNase ZS1 function,which influences ubiquitin fusion ribosomal protein L40(UbL40)messenger RNA levels during male development.Here,we used ATAC-seq,combined with analysis of H3K9ac and H3K4me2,to identify changes in accessible chromatin during fertility conversion of the two-line hybrid rice Wux-iang S(WXS)derived from a mutant tms5 allele.Fur-thermore,RNA-seq and bioinformatic analyses identified specific transcription factors(TFs)in differentially accessible chromatin regions.Among these TFs,only GATA10 targeted UbL40.Osgata10 knockout mutations,which resulted in low expression of UbL40 and a tendency toward male fertility,confirmed that GATA10 regulated fertility conversion via themodulation of UbL40.Meanwhile,GATA10 acted as a medi-ator for interactions with ERF65,which revealed that tran-scriptional regulation is a complex process involving multiple complexes of TFs,namely TF modules.It appears that the ERF141/MADS7/MADS50/MYB modules affect metabolic processes that control anther and pollen development,es-pecially cell wall formation.Our analysis revealed that these modules directly or indirectly affect metabolic pathway-re-lated genes to coordinate plant growth with proper anther development,and furthermore,that GATA10 regulates fer-tility conversion via the modulation of UbL40 expression.

关键词： sativa fertility sterile

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Women’s preconception health patterns in traditional Chinese medicine as a predictor of fertility outcomes

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Journal of Integrative Medicine 2020年第3期18卷 222-228页

作者： Xia-qiu Wu Wendy Satmary Jin Peng Ka-kit Hui School of Basic Medical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina Center for East-West Medicine David Geffen School of MedicineUniversity of CaliforniaLos AngelesCA 90024USA Department of Obstetrics&Gynecology David Geffen School of MedicineUniversity of CaliforniaLos AngelesCA 90024USA Institute of Basic Research in Clinical Medicine China Academy of Chinese Medical SciencesBeijing 100700China

Objective: To examine the association between traditional Chinese medicine(TCM), preconception health patterns and fertility outcomes.Methods: A community-based prospective cohort study was conducted in China. A total of 3012 newly married women who were willing to conceive within 2 years were enrolled in the study and took National Free Prepregnancy Checkups(NFPC). A reliably structured self-rating scale was used to measure the TCM preconception health patterns of the enrolled women. A 3-year follow-up was conducted to obtain the fertility outcomes, including pregnancy rate, time to pregnancy, spontaneous miscarriage and newborn status. Statistical analyses were conducted using Chi-square or Fisher’s exact tests, logistic regression models, general linear models and the Cox proportional hazard model.Results: The fertility outcomes showed no statistic correlations to the terms of NFPC in this population.Approximately a half of the women(46.66%) had unhealthy patterns. Women with qi & blood-deficiency(odds ratio [OR] = 35.19, 95% confidence interval [CI] = 1.55–801.15) or qi-stagnation(OR = 4.55, 95% CI =0.90–23.06) pattern took a longer time to get pregnant, and those with qi-stagnation(OR = 2.05, 95% CI =1.1–3.82) or yang-deficiency(OR = 1.91, 95% CI = 1.12–3.25) pattern had a higher risk of spontaneous miscarriage.Conclusion: Three unhealthy TCM patterns during the preconception period might be risk factors for low fecundity or poor pregnancy outcomes. The TCM preconception pattern identification may provide a convenient and effective way to screen for potential pregnancy risks beyond the NFPC. Further, appropriate interventions based on the TCM preconception health patterns are needed to improve quality in women’s fecundability and birth outcomes.

关键词： Preconception care fertility Traditional Chinese medicine Women’s health Risk assessment

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The deubiquitinating gene Usp29 is dispensable for fertility in male mice

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Science China(Life Sciences) 2019年第4期62卷 544-552页

作者： Zhu Huang Manan Khan Jianze Xu Teka Khan Hui Ma Ranjha Khan Hafiz Muhammad Jafar Hussain Xiaohua Jiang Qinghua Shi Hefei National Laboratory for Physical Sciences at Microscale The First Affiliated Hospital of USTC School of Life Sciences CAS Center for Excellence in Molecular Cell Science University of Science and Technology of China(USTC) Collaborative Innovation Center of Genetics and Development The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui Life Science College of Anqing Normal University Department of Zoology Abbottabad University of Science and Technology

The balanced actions between ubiquitination and deubiquitination precisely control the levels of various proteins vital for spermatogenesis. Ubiquitin-specific processing proteases(USPs) are the largest family of deubiquitinatingenzymes(DUBs),containing more than 50 members. So far, the functions of only a few USPs in male fertility have been studied, the roles of the majority are yet unknown. The present study aimed to explore the function of Usp29(ubiquitin-specific protease 29) in male fertility. We found that Usp29 showed predominant expression in mouse testis, and its m RNA expression started to increase at 14 days postpartum(dpp), with a peak at 28 and 35 dpp. Using CRISPR/Cas9 technology, we generated Usp29 knockout mice(Usp29^(–/–)). Usp29^(–/–)mice exhibited no overt developmental anomalies. Further examination revealed that Usp29^(–/–)mice had normal fertility and showed no detectable difference in the testis/body weight ratio, testicular and epididymal histology as well as epididymal sperm count from the wild-type littermates. Moreover, Usp29 is not a pseudogene in mice. Taken together, our study first reported that though Usp29 is predominantly expressed in the testis, it is not essential for male fertility in mice.

关键词： Usp29 mouse testis fertility

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Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

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Journal of Animal Science and Biotechnology 2020年第1期11卷 44-57页

作者： MariaPortia B.Nagata Junki Egashira Naoto Katafuchi Kenji Endo Kazuko Ogata Kenichi Yamanaka Tadayuki Yamanouchi Hideo Matsuda Yutaka Hashiyada Kenichi Yamashita Advanced Manufacturing Research Institute National Institute of Advanced Industrial Science and Technology(AIST)807-1 Shuku-machiTosuSaga 841-0052Japan Saga Prefectural Livestock Experiment Station 23242-2 Yamauchi-machiMiyanoTakeoSaga 849-2305Japan Morinaga Dairy Service Co.Ltd. 1-159 ToyoharaotsuNasu-gun Nasu-machiTochigi 329-3224Japan National Livestock Breeding Center(NLBC) 1 OdakuraharaOdakuraNishigo-muraNishishirakawa-gunFukushima 961-8511Japan Institute of Livestock and Grassland Science National Agriculture and Food Research Organization(NARO)Ikenodai 2TsukubaIbaraki 305-0901Japan Faculty of Agriculture Saga University1 Honjo-machiSaga 840-8502Japan.7Ishikawa Prefectural University1-308 SuematsuNonoichi-shiIshikawa 921-8836Japan.

Background:The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal reproduction.However,a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation,rendering the frozen-thawed semen impractical for routine use.Cryopreservation is known to reduce sperm lifespan and fertility.Variation in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination(AI)centers.Our goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation process.Results:Our strategy of sperm selection based on rheotaxis and thermotaxis(SSRT)on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity,viability and biological function related to fertilization competence.This strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat breeders.This technique protected further sublethal damage to bovine spermatozoa(higher%cryosurvival than the control)and resulted in the improvement of DNA integrity.Prefreeze selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo fertilization.Conclusions:These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival,longevity of post-thawed sperm,and optimization of in vivo fertilization,embryo development and calving as supported by the favorable results of field fertility study.

关键词： Cryopreservation fertility Livestock Rheotaxis Spermatozoa Thermotaxis

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Commentary on“CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice”

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Asian Journal of Andrology 2022年第3期24卷 273-273页

作者： Brendan J Houston Bio21 Institute and The School of BioSciences The University of Melbourne Australia Parkville VIC 3052

Several thousand genes(17000–19000)are expressed in the human/mouse testis and a sizeable number of these are testis-enriched.However,the function of many of these genes in spermatogenesis is yet to be investigated.In a recent study by Oyama et al.,1 the essential nature of 12 testis-enriched genes in male fertility was tested using knockout mouse models generated by clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)technology.The authors performed basic male fertility analyses for three knockout lines(Tmem210,4921539E11Rik,and 4930558C23Rik)and determined that spermatogenesis was normal,motile and morphologically normal sperm were produced,and knockout males were fertile.For the remaining nine lines(Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tomm20l,and 1700057G04Rik),the authors simply investigated fertility via mating.Thus,there is a small chance that subtle roles of these genes in spermatogenesis or optimal fertility were overlooked.Altogether,despite a biased testis expression,each gene was dispensable for male fertility.

关键词： CRISPR/Cas9 fertility enriched

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Effect of testicular capsulotomy on fertility of rats

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Asian Journal of Andrology 2001年第1期3卷 21-25页

作者： Da-NianQIN MaryA.Lung DepartmentofPhysiology ShantouUniversitySchoolofMedicineShantou515031China DepartmentofPhysiology UniversityofHongKongHongKongChina

Aim: To demonstrate the effect of capsulotomy on the fertility of male rats. Methods: Testicular capsulotomywas carded out in immature (21 days) and adult (60 days) male rats. The fertility of them was assessed by cohabita-tion with proestrus females overnight and 20 days later, the females were examined for impregnation. Morphologicalchanges at the site of the capsulotomy were observed under light microscope. Results: In rats capsulotomized atDay 60, the fertility was gradually depressed and all the rats completely lost their fertility 2 months post-operation. Atthat time, a partial regeneration of the capsule at the site of capsulotomy was observed. Immature rats capsulotomized atDay 21 were found to possess normal fertility at maturity. The capsulotomy site was almost completely recovered 60days post-operation. Conclusion: In male rats, testicular capsulotomy at the age of Day 60 will damage fertility.However, when capsulotomy is performed at Day 21, fertility is preserve.(Asian J Androl 2001 Mar; 3: 21-25)

关键词： testis testicular capsulotomy fertility

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Semen quality analysis and the idea of normal fertility

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Asian Journal of Andrology 2010年第1期12卷 79-82页

作者： Michael Joffe Department of Epidemiology and Public Health Imperial College London St Mary＇s Campus London W2 1PF UK

The World Health Organization （WHO） has extensively revised its manual for semen analysis, and seeks to provide reference limits for semen quality parameters. This raises the question of what is meant by ＇normal＇（excluding the use of this term to denote a Gaussian distribution）. It could be taken in a purely statistical sense, using a biologically arbitrary cut-off point to denote an abnormal level, typically the extreme 5 percent of the population. Alternatively, ＇normal＇ could be defined according to the biological concept of normality and abnormality, in terms of the point at which biological function becomes impaired. Either of these can be used in descriptive epidemiology, for example, to study trends, but in the case of fertility, both semen quality and functional fertility （time to pregnancy） are continuous variables with no clear threshold. The WHO manual uses the biological meaning of normal, in that it provides the semen parameter distributions for men who have recently fathered pregnancies that took 12 months or less to conceive. However, what is really needed is the same information the other way around： given a particular semen test result, what should be expected in terms of ability to conceive, and how long it is likely to take. In considering epidemiological research, the focus has been mainly on internal comparisons, rather than reference limits, but it would be beneficial if more attention were paid to the absolute levels and to what these mean in terms of function--in other words, if the data were better calibrated biologically.

关键词： fecundability fecundity fertility semen semen analysis subfecundity

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Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L

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Horticulture Research 2020年第1期7卷 1971-1980页

作者： Wenjing Ren Zhiyuan Li Fengqing Han Bin Zhang Xing Li Zhiyuan Fang Limei Yang Mu Zhuang Honghao Lv Yumei Liu Yong Wang Hailong Yu Yangyong Zhang Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural CropsMinistry of Agriculture#12 Zhong Guan Cun Nandajie StreetBeijing 100081China

Clubroot disease,a major plant root disease caused by Plasmodiophora brassicae,has become one of the most destructive diseases among cultivated cruciferous vegetables.However,clubroot-resistant Brassica oleracea materials are rare.A few clubroot-resistant cabbage varieties are available on the market,but all are Ogura cytoplasmic male sterile(CMS)types.Therefore,in this study,to reutilize the clubroot-resistant Ogura CMS germplasm of cabbage,a new fertility-restored Ogura CMS material,16Q2-11,was used as a bridge to transfer the clubroot resistance(CR)gene from the Ogura CMS cytoplasm to the normal cytoplasm by a two-step method(a fertility restoration and cytoplasm replacement method).In the first cross for fertility restoration of Ogura CMS clubroot-resistant cabbage(FRCRC),16Q2-11 was used as a restorer to cross with Ogura CMS materials containing the CR gene CRb2.Eleven Rfo-positive progenies were generated,of which four contained CRb2:F8-514,F8-620,F8-732 and F8-839.After inoculation with race 4 of P.brassicae,these four CRb2-positive individuals showed resistance.Furthermore,F8-514 and F8-839 were then used as male parents in the second cross of FRCRC to cross with cabbage inbred lines,resulting in the successful introgression of the CRb2 gene into the inbred lines.All offspring produced from this step of cross,which had a normal cytoplasm,showed a high resistance to race 4 of P.brassicae and could be utilized for the breeding of clubrootresistant cabbage varieties in the future.This is the first time that the Ogura CMS restorer has been used to restore the fertility of Ogura CMS clubroot-resistant cabbages,which could improve germplasm diversity in cabbage and provide a reference method for using CMS germplasm in Brassica crops.

关键词： fertility germplasm breeding

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Negative biomarker-based male fertility evaluation： sperm phenotypes associated with molecular-level anomalies

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Asian Journal of Andrology 2015年第4期17卷 554-560页

作者： Peter Sutovsky Mahmoud Aarabi Antonio Miranda-Vizuete Richard Oko Division of Animal Science and Departments of Obstetrics Gynecology and Women's Health University of Missouri Columbia Missouri USA Department of HumanGenetics McGill University Quebec Canada Department of Biomedical and Molecular Sciences Institute de Biomedicina de Sevilla Hospital Universitario Virgen delRocb/CSIC/Universidad de Sevilla Sevilla Spain Queens University Kingston Ontario Canada.

Biomarker-based sperm analysis elevates the treatment of human infertility and ameliorates reproductive performance in livestock. The negative biomarker-based approach focuses on proteins and ligands unique to defective spermatozoa, regardless of their morphological phenotype, lending itself to analysis by flow cytometry （FC）. A prime example is the spermatid specific thioredoxin SPTRX3/TXNDC8, retained in the nuclear vacuoles and superfluous cytoplasm of defective human spermatozoa. Infertile couples with high semen SPTRX3 are less likely to conceive by assisted reproductive therapies （ART） and more prone to recurrent miscarriage while low SPTRX3 has been associated with multiple ART births. Ubiquitin, a small, proteolysis-promoting covalent posttranslational protein modifier is found on the surface of defective posttesticular spermatozoa and in the damaged protein aggregates, the aggresomes of spermiogenic origin. Semen ubiquitin content correlates negatively with fertility and conventional semen parameters, and with sperm binding of lectins LCA （Lens culinaris agglutinin; reveals altered sperm surface） and PNA （Arachis hypogaealpeanut agglutinin; reveals acrosomal malformation or damage）. The Postacrosomal Sheath WWl Domain Binding Protein （PAWP）, implicated in oocyte activation during fertilization, is ectopic or absent from defective human and animal spermatozoa. Consequently, FC-parameters of PAWP correlate with ART outcomes in infertile couples and with fertility in bulls. Assays based on the above biomarkers have been combined into multiplex FC semen screening protocols, and the surface expression of lectins and ubiquitin has been utilized to develop nanoparticle-based bull semen purification method validated by field artificial insemination trials. These advances go hand-in-hand with the innovation of FC-technology and genomics/proteomics-based biomarker discovery.

关键词： artificial insemination biomarker fertility fertilization flow cytometry infertility nanotechnology oocyte activation Postacrosomal Sheath WWI Domain Binding Protein sperm SPTRX3 thioredoxin ubiquitin

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Variance components and correlations of female fertility traits in Chinese Holstein population

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Journal of Animal Science and Biotechnology 2017年第4期8卷 833-841页

作者： Aoxing Liu Mogens Sandø Lund Yachun Wang Gang Guo Ganghui Dong Per Madsen Guosheng Su Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture of China National Engineering Laboratory of Animal BreedingCollege of Animal Science and Technology China Agricultural UniversityBeijing 100193 China Center for Quantitative Genetics and Genomics Department of Molecular Biology and Genetics Aarhus University 8830 TjeleDenmark. Beijing Sunlon Livestock Development Co. Ltd Beijing 100176 China

Background: The objective of the present study was to estimate(co)variance components of female fertility traits in Chinese Holsteins, considering fertility traits in different parities as different traits. Data on 88,647 females with 215,632 records(parities) were collected during 2000 to 2014 from 32 herds in the Sanyuan Lvhe Dairy Cattle Center, Beijing, China. The analyzed female fertility traits included interval from calving to first insemination, interval from first to last insemination, days open, conception rate at first insemination, number of inseminations per conception and non-return rates within 56 days after first insemination.Results: The descriptive statistics showed that the average fertility of heifers was superior to that of cows. Moreover,the genetic correlations between the performances of a trait in heifers and in cows were all moderate to high but far from one, which suggested that the performances of a trait in heifers and cows should be considered as different but genetically correlated traits in genetic evaluations. On the other hand, genetic correlations between performances of a trait in different parities of cows were greater than 0.87, with only a few exceptions, but variances were not homogeneous across parities for some traits. The estimated heritabilities of female fertility traits were low; all were below 0.049(except for interval from calving to first insemination). Additionally, the heritabilities of the heifer interval traits were lower than those of the corresponding cow interval traits. Moreover, the heritabilities of the interval traits were higher than those of the threshold traits when measuring similar fertility functions. In general, estimated genetic correlations between traits were highly consistent with the biological categories of the female fertility traits.Conclusions: Interval from calving to first insemination, interval from first to last insemination and non-return rates within 56 days after first insemination are recommended to be included in the selection in

关键词： Chinese Holsteins Female fertility Genetic correlation Heritability

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