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Journal of Ocean University of China 2007年第2期6卷 187-192页

作者： YU Shanshan KONG Xiaoyu LI Yulong XU Hui Key Laboratory of Mariculture of Ministry of Education Ocean University of China Qingdao 266003 P. R. China

The complete mitochondrial cytochrome oxidase subunit Ⅱ （COⅡ） gene of Penaeinae shrimp fenneropenaeus chinensis was cloned and sequenced. The gene is 688 bp in length and codes for 229 amino acids. It shows 83.2%, 87.0% and 83.8% sequence similarity to Marsupenaeus Japonicus, Penaeus monodon and Farfantepenaeus notialis, respectively. The A＋T content of the whole gene and that at the third position of codons are 64.7% and 78.2%, respectively. The phylogenetic relationship between F. chinensis and three other species representing genera Farfanatepenaeus, Marsupenaeus and Penaeus was analyzed. Results showed that the genetic distances among the four taxa ranged from 0.144 0 to 0.200 5, exceeding those estimated with COⅠ and partial 16S rRNA gene sequences among Marsupenaeus, Litopenaeus and Melicertus, and being therefore larger than the value among subgenera. It has been suggested that the COⅡ gene has a faster evolutionary rate than that of the COⅠ gene and partial 16S rRNA gene and could be used for phylogenetic analysis at genus or species level. The results of the present study indicated that Farfantepenaeus, fenneropenaeus, Marsupenaeus and Penaeus are at a higher phylogenetic level than subgenus, which supports the opinion of the elevation of phylogenetic status of the four subgenera to genus level.

关键词： fenneropenaeus chinensis COⅡ gene phylogenetic analysis

Research in migration route of hatchery released Chinese shrimp(fenneropenaeus chinensis) in the Bohai Bay using method of SSR marker

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Acta Oceanologica Sinica 2020年第12期39卷 76-81页

作者： Weiji Wang Ding Lyu Mosang Wang Kefeng Liu Jie Kong Xiujuan Shan Xianshi Jin Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Bohai Fisheries Research Institute of Tianjin Tianjin 300457China

This study provides new insights for the hatchery released Chinese shrimp(fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than *** shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in *** 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery ***,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering *** unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.

关键词： fenneropenaeus chinensis migration route hatchery release stock enhancement SSR marker

Effects of salinity fluctuation amplitudes on growth,osmolarity,Na^+-K^+-ATPase activity and Hsp70 of juvenile Chinese shrimp fenneropenaeus chinensis Osbeck

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Chinese Journal of Oceanology and Limnology 2009年第4期27卷 723-728页

作者：丁森王芳董双林高勤峰 The Key Laboratory of Mariculture Ministry of EducationOcean University of China

The effects of various salinity fluctuation amplitudes （2, 4, 6 and 8） on the growth, osmolarity, Na＋-K＋-ATPase activity and Hsp70 of juvenile fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied. The results show that weight gain in the salinity fluctuation treatments was better than that in control; in particular, the weight gain of treatments S4 and SO, at 231.8% and 196.3%, respectively, was significantly different （P〈0.05）. The hemolymph osmolarity of treatments SO, S2, S4, S6 and S8 was 635.4, 630.8, 623.6, 614.4 and 600.3 mOsm/kg, respectively, and decreased with increasing salinity fluctuation amplitude. The level of Na＋-K＋-ATPase activity in gills ofE chinensis was higher than that in hepatopancreas, but there were no significant differences among all treatments, either in gills or hepatopancreas （P〉0.05）. The relative level of Hsp70 in treatment $4 was 48.4% and 40.4% higher than control in muscle and eyestalks, respectively, with the highest values being recorded under a salinity fluctuation amplitude of 4.

关键词： fenneropenaeus chinensis growth osmolarity Na+-K+-ATPase activity Hsp70 salinityfluctuation amplitude

Genetic improvement on Chinese shrimp (fenneropenaeus chinensis):growth and viability performance in F1 hybrids of different populations

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Chinese Journal of Oceanology and Limnology 2008年第4期26卷 369-374页

作者：田燚孔杰李文东栾生杨翠华王清印 Dalian Fisheries University Yellow Sea Fisheries Research Institute Institute of Oceanology Chinese Academy of Sciences Graduate School Chinese Academy of SciencesBeijingChina Ocean University of China

fenneropenaeus chinensis distributed in the Yellow Sea and Bohai Sea of China and the west coast of the Korean Peninsula. Different geographical populations represent potentially different genetic resources. To learn further the characteristics of different geographical population, crosses among two wild and three farmed populations were produced. The two wild populations were from the Yellow Sea and Bohai Sea （WYP）, and the west coast of the Korean Peninsula and coast （WKN）. The three farmed populations included the offspring of first generation of wild shrimp from coast in Korea （FKN）, the Huang Hai （the Yellow Sea in Chinese） No.1 （HH1）, and JK98. The phenotypes growth and survival rates of these populations were compared to confirm the feasibility for crossbreeding. The body length （BL）, carapace length （CL）. carapace width （CW）, height of the second and third abdominal segment （HST）, width of the second and third abdominal segment （WST）, length of the first abdominal segment （LF）, length of the last abdominal segment （LL）, live body weight （BW）, and survival rate were measured, Different combinations were statistically performed with ANOVA and Duncan＇s Multiple Range Test. The results show that the survival rate of JK98（♀）×WKN（♂） was the highest, followed by WYP（♀）×WKN（♂）, FKN（♀）×WYP（♂）, FKN（♀）×HHI（♂） and WYP（♀）×FKN（♂）; the body weight of FKN（♀）×HHI（♂） was the highest, followed by FKN（♀）×WYP（♂）, WYP（♀）×WKN（♂）, WYP（♀）×FKN（♂） and JK98（♀）×WKN（♂）; the total length had the same ranking as the body weight. All growth traits in hybrids JK98（♀）×WKN（♂） were the lowest among all combinations. F1 hybrids had significant difference （P〈0.05） in BL, CL, HST, LL, and BW; and insignificant difference （P〉0.05） in other growth traits and survival rate. The results of Duncan＇s Multiple Range Test are that BL and CL of JK98（♀）×WKN（♂） were significantly different from the other combinations; HST different from the combination of FKN（♀）×WYP（♂）, FKN（♀）×HHI（♂） and WYP（♀）×WKN（♂）; and B

关键词： fenneropenaeus chinensis population hybridization growth performance viability

Expression profiles of penaeidin from fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR

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Acta Oceanologica Sinica 2005年第2期24卷 131-140页

作者： DONGBo LIUFengsong XIANGJianhai LIFuhua GAOHongwei ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China／／GraduateSchoolChineseAcademyofSciencesBeijing100039China ExperimentalMarineBiologylaboratory InstituteofOceanologyChineseAcademyofSciencesQingdao266071China QingdaoInspectionandQuarantineBureau Qingdao266101China

Penaeidin from Chinese shrimp (fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp.

关键词： penaeidin fenneropenaeus chinensis expression profile WSSV Vibrio anguillarum real-time PCR

Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (fenneropenaeus chinensis)

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Chinese Journal of Oceanology and Limnology 2010年第1期28卷 37-45页

作者：周倩如邵明瑜秦贞奎康庆浩张志峰 Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China Department of Aquaculture Chonnam National University

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication, DNA repair, and RNA processing. However, the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level. We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins, Fc-vasa and Fc-PL10a, from the testes of Chinese shrimp, fenneropenaeus chinensis. The two predicted amino acid sequences contain all the conserved motifs characterized by the DEAD-box family and several RGG repeats in the N-terminal regions. Homology and phylogenetic analyses indicate that they belong to the vasa and PLIO subfamilies. The three-dimensional structures of the two proteins were predicted with a homology modeling approach. Both core proteins consist of two tandem RecA-like domains similar to those of the DEAD-box RNA helicase. Using reverse transcription-polymerase chain reaction （RT-PCR） and real-time PCR we found that Fc-vasa was expressed specifically in the adult gonads. Transcription decreased in the ovary but increased in the testis during gonadal development. Fc-PL10a expression was widely distributed in the tissues we examined. Using in situ hybridization, we demonstrated that the Fc-vasa transcript is localized to the cytoplasm of the spermatogonia and oocytes. Thus, our results suggest that Fc-wasa plays an important role in germ-line development, and has utility as a germ cell lineage marker which will help to generate new insight into the origin and differentiation of germ cells as well as the regulation of reproduction in F. chinensis.

关键词： DEAD-box fenneropenaeus chinensis germ-line vasa PL10

Parent-offspring relationship recognition based on SSR and mtDNA confirmed resource supplement effect of fenneropenaeus chinensis release

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Acta Oceanologica Sinica 2024年第2期43卷 156-160页

作者： Song Sun Ding Lyu Xianshi Jin Xiujuan Shan Weiji Wang Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The resource of fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and *** order to supplement the fishing yield and restore resources of ***,the relevant authorities have carried out the activities of stock enhancement and *** can increase biomass and recover ***,compared with increasing biomass,there were still few reports on its effect on the recovery of *** recovery is a process related to whether the released individuals can form a reproductive *** to now,there has been a lack of evidence whether the released *** can complete the entire life history,and form reproduction *** this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow *** identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture *** was confirmed for the first time that at least part of the released *** can complete overwintering and reproductive migration,and maintain the migration habits as their wild ***,we infered that the released shrimp can reproduce under natural conditions,these *** can form reproductive populations theoretically if without human *** results indicated that enhancenment and release activities have a positive effect on resource recovery.

关键词： fenneropenaeus chinensis release simple sequence repeat(SSR) mtDNA resource supplement

Genetic parameters for cold tolerance and body weight of Chinese fleshy prawn,fenneropenaeus chinensis

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Acta Oceanologica Sinica 2016年第6期35卷 12-18页

作者： LI Wenjia LUAN Sheng LUO Kun SUI Juan LU Xia WANG Qingyin KONG Jie Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology

The inability of fenneropenaeus chinensis to tolerate low temperatures is of major economic concern in temperate climates,as it reduces their growing season and leads to over-winter *** this study,the heritability of body weight under low grow-out temperature and cold tolerance in *** were first investigated and estimated using 88 ful-sib families,which might provide crucial information in Chinese fleshy prawn breeding *** heritability for body weight under suitable and low temperature of *** were both moderate（0.158 0±0.307 5 and 0.132 0±0.026 9 respectively）;the large coefficient of variation（approximately 21%） and moderate estimate of heritability for body weight indicated substantial potential for selective *** heritability estimate for cold tolerance was low（0.019 2±0.023 5）,and showed no significant differences from zero（P〉0.05）.A weak genetic correlation between cold tolerance and body weight was also estimated in the present study,also showing no significant differences from zero（P〉0.05）.Thus,more research needs to be conducted on the more accurate heritability estimate of cold tolerance and genetic correlations between traits in *** to further improve the achievement of breeding goals.

关键词： fenneropenaeus chinensis heritability body weight cold tolerance genetic correlation

Effects of salinity fluctuation in different ranges on the intermolt period and growth of juvenile fenneropenaeus chinensis

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Acta Oceanologica Sinica 2005年第3期24卷 141-147页

作者： MUYingchun WANGFang DONGShuanglin DONGShaoshuai ZHUChangbo KeyLaboratoryofMaricultureofMinistryofEducation FisheriesCollegeDepartmentofLifeSciencesandTechnologyOceanUniversityofChinaQingdao266003China

Experiments were conducted to examine the effects of salinity fluctuation on the intermolt period and growth of fenneropenaeus chi- nensis with an initial body weight of (0.553 2±0.000 1)g (mean plus or minus SE). The salinity (S0) of the control treatment is 30 throughout the experiment, while the treatments S2, S4, S7 and S10 are subjected to different salinity fluctuations, and the ranges are 2, 4, 7 and 10, respectively. After a 30-day feeding trial, the longest and the shortest intermolt periods of the shrimp occur under Treatments S10 and S2, respectively (difference 37.14%). The special growth rates (SGR) of the shrimp under the five treatments are ranked as S7> S4> S2> S10> S0, and the SGR of the shrimp under Treatment S0 is lower than that under Treatments S4 and S7 by 22.36% and 28.11%, respectively (P 0.05).

关键词： fenneropenaeus chinensis salinityfluctuation intermolt period growth

Discovery of immune related factors in fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2 , XIANG Jianhai 1*, WANG Bing 1,2 , LI Fuhua 1 and TONG Wei 3(1. Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071,China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China 3. Genomics & Bioinformatics Institute, Chinese Academy of Sciences, Beijing 101300, China) Chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China Chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： fenneropenaeus chinensis bioinformatics ESTs immune factors