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Leucosceptroid B from glandular trichomes of Leucosceptrum canum reduces fat accumulation in Caenorhabditis elegans through suppressing unsaturated fatty acid biosynthesis

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Chinese Journal of Natural Medicines 2019年第12期17卷 892-899页

作者： LING Yi TENG Lin-Lin HUA Juan LI De-Sen LUO Shi-Hong LIU Yan-Chun LIU Yan LI Sheng-Hong State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Yunnan Key Laboratory of Natural Medicinal Chemistry Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Obesity that is highly associated with numerous metabolic diseases has become a global health issue *** sesterterpenoids are an important group of natural products with great potential;thus,their bioactivities deserve extensive ***-seq analysis indicated that leucosceptroid B,a sesterterpenoid previously discovered from the glandular trichomes of Leucosceptrum canum,significantly regulated the expression of 10 genes involved in lipid metabolism in Caenorhabditis ***,leucosceptroid B was found to reduce fat storage,and downregulate the expression of two stearoyl-CoA desaturase(SCD)genes fat-6 and fat-7,and a fatty acid elongase gene elo-2 in wild-type *** addition,leucosceptroid B significantly decreased fat accumulation in both fat-6 and fat-7 mutant worms but did not affect the fat storage of fat-6;fat-7 double *** findings indicated that leucosceptroid B reduced fat storage depending on the downregulated expression of fat-6,fat-7 and elo-2 and thereby inhibiting the biosynthesis of the corresponding unsaturated fatty *** findings provide new insights into the development and utilization of plant sesterterpenoids as potential antilipemic agents.

关键词： Sesterterpenoid Leucosceptroid B Leucosceptrum canum Caenorhabditis elegans Fat accumulation fatty acid biosynthesis Antilipemic agent

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HCV NS5A and NS5B Enhance Expression of Human Ceramide Glucosyltransferase Gene

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Virologica Sinica 2012年第1期27卷 38-47页

作者： Jia Guo Ran Yan Guo-dong Xu Cong-yi Zheng State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China China Center for Type Culture Collection Wuhan UniversityWuhan 430072China

Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus （HCV） infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a positive effect on the replication of the HCV full-length replicon when cells were treated with nystatin. Conversely, the HCV RNA replication was decreased when fatty acid biosynthesis was inhibited with 25-hydroxycholesterol and PDMP（D-threo-l-phenyl-2-decanoylamino-3- morpholino-l-propanol）. In agreement with these results, the expression level of GlcT-l（ceramide glucosyltransferase）, a host glucosyltransferase in the first step of GSL （glycosphingolipid） biosynthesis, was found to be closely associated with the expression and replication of HCV RNA. On the other hand, the viral RNA can also activate GlcT-1 in the early stage of viral RNA transfection in vitro. To identify viral factors that are responsible for GlcT-1 activation, we constructed ten stable Vero cell lines that express individual HCV proteins. Based on the analyses of these cell lines and transient transfection assay of the GlcT-1 promoter regions, we conclude that HCV proteins, especially NS5A and NS5B, have positive effects on the expression of GlcT-1. It is possible that NS5A and NS5B stimulate transcription factor（s） to activate the expression of GlcT-1 by increasing its transcription level

关键词： Hepatitis C virus fatty acid biosynthesis Ceramide glucosyltransferase Stable cell lines

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Chain Elongation Using Native Soil Inocula:Exceptional n-Caproate biosynthesis Performance and Microbial Mechanisms

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Engineering 2024年第8期39卷 262-272页

作者： Lin Deng Yang Lv Tian Lan Qing-Lian Wu Wei-Tong Ren Hua-Zhe Wang Bing-Jie Ni Wan-Qian Guo State Key Laboratory of Urban Water Resource and the Environment Harbin Institute of TechnologyHarbin 150090China Centre for Technology in Water and Wastewater&School of Civil and Environmental Engineering University of Technology SydneySydneyNSW 2007Australia

This study demonstrates the feasibility and effectiveness of utilizing native soils as a resource for inocula to produce n-caproate through the chain elongation(CE)platform,offering new insights into anaerobic soil *** results reveal that all five of the tested soil types exhibit CE activity when supplied with high concentrations of ethanol and acetate,highlighting the suitability of soil as an ideal source for n-caproate *** with anaerobic sludge and pit mud,the native soil CE system exhibited higher selectivity(60.53%),specificity(82.32%),carbon distribution(60.00%),electron transfer efficiency(165.00%),and conductivity(0.59 ms∙cm^(-1)).Kinetic analysis further confirmed the superiority of soil in terms of a shorter lag time and higher yield.A microbial community analysis indicated a positive correlation between the relative abundances of Pseudomonas,Azotobacter,and Clostridium and n-caproate ***,metagenomics analysis revealed a higher abundance of functional genes in key microbial species,providing direct insights into the pathways involved in n-caproate formation,including in situ CO_(2)utilization,ethanol oxidation,fatty acid biosynthesis(FAB),and reverse beta-oxidation(RBO).The numerous functions in FAB and RBO are primarily associated with Pseudomonas,Clostridium,Rhodococcus,Stenotrophomonas,and Geobacter,suggesting that these genera may play roles that are involved or associated with the CE ***,this innovative inoculation strategy offers an efficient microbial source for n-caproate production,underscoring the importance of considering CE activity in anaerobic soil microbial ecology and holding potential for significant economic and environmental benefits through soil consortia exploration.

关键词： Soil Chain elongation n-caproate Reverse beta-oxidation fatty acid biosynthesis Metagenomics

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