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Dietary methionine deficiency stunts growth and increases fat deposition via suppression of fatty acids transportation and hepatic catabolism in Pekin ducks

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1871-1886页

作者： Yongbao Wu Jing Tang Zhiguo Wen Bo Zhang Junting Cao Lulu Zhao Zhanbao Guo Ming Xie Zhengkui Zhou Shuisheng Hou State Key Laboratory of Animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China

Background:Although methionine(Met),the first-limiting dietary amino acid,has crucial roles in growth and regulation of lipid metabolism in ducks,mechanisms underlying are not well understood.Therefore,the objective was to use dietary Met deficiency to investigate the involvement of Met in lipid metabolism and fat accumulation of Pekin ducks.Methods:A total of 150 male Pekin ducks(15-d-old,558.5±4.4 g)were allocated into 5 groups(6 replicates with 5 birds each)and fed corn and soybean meal-based diets containing 0.28%,0.35%,0.43%,0.50%,and 0.58%Met,respectively,for 4 weeks.Met-deficient(Met-D,0.28%Met)and Met-adequate(Met-A,0.43%Met)groups were selected for subsequent molecular studies.Serum,liver,and abdominal fat samples were collected to assess the genes and proteins involved in lipid metabolism of Pekin ducks and hepatocytes were cultured in vivo for verification.Results:Dietary Met deficiency caused growth depression and excess fat deposition that were ameliorated by feeding diets with adequate Met.Serum triglyceride and non-esterified fatty acid concentrations increased(Pfatty acid transport,fatty acid oxidation,tricarboxylic acid cycle,glycolysis/gluconeogenesis,ketogenesis,and electron transport chain;selected key proteins had similar expression patterns verified by qRT-PCR and Western blotting,which indicated these processes were likely impaired.In vitro verification with hepatocyte models confirmed albumin expression was diminished by Met deficiency.Additionally,in abdominal fat,dietary Met deficiency increased adipocyte diameter and area(P<0.05),and down-regulated(P<0.05)of lipolytic genes and proteins,suggesting Met deficiency may suppress lipolysis in adipocyte.Conclusion:Taken together,these data demonstrated th

关键词： fat deposition Hepatic catabolism Methionine deficiency Pekin duck Proteomics

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Identification of key genes affecting porcine fat deposition based on coexpression network analysis of weighted genes

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Journal of Animal Science and Biotechnology 2022年第1期13卷 36-51页

作者： Kai Xing Huatao Liu Fengxia Zhang Yibing Liu Yong Shi Xiangdong Ding Chuduan Wang Animal Science and Technology College Beijing University of AgricultureBeijingChina Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of AgricultureNational Engineering Laboratory for Animal BreedingCollege of Animal Science and TechnologyChina Agricultural UniversityBeijingChina

Background:fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition.

关键词： fat deposition Pigs RNA-seq WGCNA

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A 314-bp SINE insertion in the ZNF2 promoter region may act as a repressor related to regulation of fat deposition in pigs

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Journal of Integrative Agriculture 2023年第2期22卷 526-536页

作者： GU Hao DU Zhan-yu Eduard MURANI Enrico D'ALESSANDRO CHEN Cai WANG Xiao-yan MAO Jiu-de Klaus WIMMERS SONG Cheng-yi College of Animal Science&Technology Yangzhou UniversityYangzhou 225009P.R.China Leibniz Institute for Farm Animal Biology(FBN) Dummerstorf 18196Germany Department of Veterinary Science Unit of Animal ProductionUniversity of MessinaMessina 98168Italy Bond Life Science Center University of MissouriColumbiaMO65211USA

Retrotransposons,a type of DNA fragment that can mobilize itself on genome,can generate genetic variations and develop for molecular markers based on the insertion polymorphism.Zinc finger proteins(ZNFs)are among the most abundant proteins in eukaryotic animals,and their functions are extraordinarily diverse and particularly important in gene regulation.In the current study,bioinformatic prediction was performed to screen for retrotransposon insertion polymorphisms(RIPs)in six ZNF genes(ZNF2,ZNF3,ZNF7,ZNF8,ZNF10 and ZNF12).Six RIPs in these ZNFs,including one short interspersed nuclear element(SINE)RIP in intron 1 and one long interspersed nuclear element 1(L1)RIP in intron 3 of ZNF2,one SINE RIP in 5′flanking region and one SINE RIP in intron 2 of ZNF3,one SINE RIP in 3′UTR of ZNF7 and one L1 RIP in intron 2 of ZNF12,were discovered and their presence was confirmed by PCR.The impact of the SINE RIP in the first intron of ZNF2,which is close to the core promoter of ZNF2,on the gene activity was investigated by dual-luciferase assay in three cell lines.Our results showed that the SINE insertion in the intron 1 of ZNF2 repressed the core promoter activity extremely significantly(Pfat thickness in Yorkshire pigs.The corrected back fat thickness of individuals with SINE insertion in the first intron of ZNF2 was significantly(Pfat deposition in the pig breeding.

关键词： retrotransposon ZNF2 polymorphism repressor fat deposition

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Identification and characterization of genes that control fat deposition in chickens

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Journal of Animal Science and Biotechnology 2014年第1期5卷 22-37页

作者： Hirwa Claire D'Andre Wallace Paul Xu Shen Xinzheng Jia Rong Zhang Liang Sun Xiquan Zhang Rwanda Agriculture Board Research Department Department ofAnimal Genetics Breeding and Reproduction College of Animal ScienceSouth China Agricultural University Council for Scientific and Industrial Research (CSIR) AnimalResearch Institute

Background： fat deposits in chickens contribute significantly to meat quality attributes such as juiciness, flavor, taste and other organoleptic properties. The quantity of fat deposited increases faster and earlier in the fast- growing chickens than in slow-growing chickens. In this study, Affymetrix Genechip~ Chicken Genome Arrays 32773 transcripts were used to compare gene expression profiles in liver and hypothalamus tissues of fast-growing and slow-growing chicken at 8 wk of age. Real-time RT-PCR was used to validate the differential expression of genes selected from the microarray analysis. The mRNA expression of the genes was further examined in fat tissues. The association of single nucleotide polymorphisms of four lipid-related genes with fat traits was examined in a F2 resource population. Results： Four hundred genes in the liver tissues and 220 genes hypothalamus tissues, respectively, were identified to be differentially expressed in fast-growing chickens and slow-growing chickens. Expression levels of genes for lipid metabolism （SULTIB1, ACSBG2, PNPLA3, LPL, AOAH） carbohydrate metabolism （MGAT4B, XYLB, GBE1, PGM1, HKDCl）cholesttrol biosynthesis （FDPS, LSS, HMGCR, NSDHL, DHCR24, IDI1, MEI） HSD17B7 and other reaction or pro- cesses （CYPIA4, CYP1A1, AKR1BI, CYP4V2, DDO） were higher in the fast-growing White Recessive Rock chickens than in the slow-growing Xinghua chickens. On the other hand, expression levels of genes associated with multicellular organism development, immune response, DNA integration, melanin biosynthetic process, muscle organ develop- ment and oxidation-reduction （FRZB, DMD, FUT8, CYP2C45, DHRSX, and CYP2C18） and with glycol-metabolism （GCNT2, ELOVL d, and FASN）, were higher in the XH chickens than in the fast-growing chickens, fiT-PCR validated high expression levels of nine out of 12 genes in fat tissues. The G1257069A and T1247123C of the ACSBG2 gene were significantly associated with abdominal fat weight. The G4928024A of the FASN gene were significantly associ- ated with fat band

关键词： Chicken fat deposition Genes

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Evaluation of dynamic effects of dietary medium-chain monoglycerides on performance,intestinal development and gut microbiota of broilers in large-scale production

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Animal Nutrition 2023年第3期14卷 269-280页

作者： Tao Liu Shengyue Ruan Qiufen Mo Minjie Zhao Jing Wang Zhangying Ye Li Chen Fengqin Feng College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China The Institute of Animal Husbandry and Veterinary Science Zhejiang Academy of Agricultural SciencesHangzhou 310021China Ningbo Research Institute Zhejiang UniversityNingbo 315000China Hangzhou Longyu Biotechnology Co. LtdHangzhou 310003China

Medium-chain monoglycerides(MG)have been reported to affect the productive performance,gut microbiota and health of broiler chickens reared in ideal experimental conditions at home and abroad.However,the effects of MG on performance,intestinal development and gut microbiota of chickens in large-scale farms during different feed stages remain unknown.The present study was conducted on a modern farm with a total of 12,000 yellow feathered broiler chicks that were randomly allotted to 2groups(1000 chicks/replicate,6 replicates/group)for a 70-day trial.The control group(CON group)received a basal diet,and the treated group(MG group)was fed a basal diet containing 300 mg/kg mixed MG.The results revealed that dietary MG significantly(P<0.05)increased the body weight and average feed intake,but notably reduced the feed conversion and mortality of chickens in large-scale production during the starter phase.The villus height of the duodenum in the MG group at 1,2 and 7 wk of age increased notably,and the villus height to crypt depth ratio at 1,2,5 and 10 wk of age was improved.Dietary MG decreased the serum insulin content of chickens at 5,7 and 10 wk of age,and decreased the serum lipopolysaccharide at 3 and 7 wk of age.The triglyceride level of chickens at 3,5 and 10 wk of age and the low-density lipoprotein cholesterol level of chickens at 7 and 10 wk of age in the MG group decreased notably,while the high-density lipoprotein cholesterol increased significantly.Moreover,MG supplementation selectively increased the relative abundance of genus Bacteroides(family Bacteroidaceae)and Lachnospiraceae_NK4A136_group,but decreased the content of genus Rikenellaceae_RC9_gut_group,Collinsella and family Barnesiellaceae in the cecum of chickens at 3,7 and 10 wk of age.Conclusively,these findings showed that dietary MG notably enhanced chicken performance,health and feed nutrient utilization at early ages by regulating gut microbiota,intestinal development and serum biochemical indices.

关键词： Medium-chain monoglycerides Broiler chickens Dynamic effect Gut microbiota fat deposition

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Different Transcription Profiles of SOCS-3,ob and IGF-I Genes and their Possible Correlations in Obese and Lean Pigs

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Acta Biochimica et Biophysica Sinica 2007年第4期39卷 305-310页

作者： Jiangwei WU Bo WANG Haowei ZHANG Taiyong YU Gongshe YANG* Laboratory of Animal fat deposition and Muscle Development.Northwest A&F University,Yangling 712100,China Laboratory of Animal fat deposition and Muscle Development Northwest A & F UniversityYangling 712100 China

Pig breeds have significant differences in fat deposition and muscle development ability.However,the molecular mechanism behind these differences is still unknown.In this study,the expressionpatterns of three candidate genes,suppressor of cytokine signaling 3(SOCS-3),obesity(oh)and insulin-likegrowth factor Ⅰ(IGF-1),which are involved in adipose metabolism or muscle development,were analyzed.Total RNA was extracted from dorsal subcutaneous adipose tissue and longissimus of 8-month-old Bameiand Largewhite pigs.Semiquantitative reverse transcription-polymerase chain reaction was used to deter-mine the expression levels of the SOCS-3 and ob genes in adipose tissue,and SOCS-3 and IGF-I genes inmuscle tissue.The results showed that in adipose tissue the expression level of SOCS-3 was significantlyhigher in Bamei(obese)pigs than that in Largewhite(lean)pigs(Pfat deposition and muscle development ability between obese and lean pigs.

关键词： SOCS-3 ob IGF-I fat deposition muscle development obese pigs lean pigs

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Molecular cloning and ontogenesis expression of fatty acid transport protein-1 in yellow-feathered broilers

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Journal of Genetics and Genomics 2008年第6期35卷 327-333页

作者： Yuzhen Song Jiaying Feng Lihua Zhou Gang Shu Xiaotong Zhu Ping Gao College of Animal Science South China Agricultural University Guangzhou 510642 China

fatty acid transport protein-1 （fatP-1） is one of the important transporter proteins involved in fatty acid transmembrane transport and fat deposition. To study the relationship between fatP-1 mRNA expression and fat deposition, chicken （Gallus gallus） fatP-1 sequence was first cloned by rapid amplification of cDNA ends （RACE）. Tissue samples of chest muscle, leg muscle, subcutaneous fat, and abdominal fat were collected from six male and six female broilers each, at 22 days, 29 days, and 42 days, respectively. The tissue specificity and ontogenesis expression pattern of the fatP-1 mRNA of yellow-feathered broilers was studied by real-time reverse transcription polymerase chain reaction （RT-PCR）, and the fat deposition laws in different tissues were also compared. A 2,488 bp cDNA sequence of chicken fatP-1 was cloned by RACE （GenBank accession no. DQ352834）, including 547 bp 3＇ end untranslated region （URT） and 1,941 bp open reading frame （ORF）. Chicken fatP-1 encoded 646 amino acid residues, which shared 83.9% and 83.0% identity with those of human and rat, respectively. The results of quantitative PCR demonstrated a constant fatP-1 mRNA expression level in the chest muscle and subcutaneous fat of both male and female broilers at three stages, whereas the expression level of the fatP-1 mRNA in the leg muscle at 42 days was significantly higher than that at 22 days or 29 days. In the abdominal fat of male broilers, the gene expression significantly increased with age, whereas the female broilers showed a dramatic downregulation of fatP-1 expression in abdominal fat at 42 days. This suggested a typical tissue-and gender-specific expression pattern of chicken fatP-1, mediating the specific process of fatty acid transport or utilization in muscle and adipose tissues.

关键词： yellow-feathered broiler fatP-1 RACE quantitative PCR fat deposition

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Feeding a natural plant extract affects fat metabolism and changes the expression of the gene for fatty acid binding protein in beef cattle

Feeding a natural plant extract affects fat metabolism and c...

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第七届草食动物营养国际学术研讨会

作者： C.R.Ge~(1 2) X.Zhang~1 J.J.Jia~(1*) S.Z.Gao~1 C.Wang~1 F.D.Li~2 M.Jois~3 ~1 Yunnan Provincial Key laboratory of Animal Nutrition and Feed Science Yunnan Agricultural University Kunming 650201 Yunnan Province China ~2 Gansu Agricultural University Lanzhou 730070 Gansu Province China ~3 Department of Agriculture School of Life Sciences La Trobe University VIC 3086 Australia

＜正＞Growing crossbred beef cattle steers were fed pasture hay ad libitum and 2.5 kg/day of a maize/soya bean based concentrate.Half the steers were offered a supplement of a dried extract of a mixture of ‘medicinal herbs’（NPE）at the rate of 6g supplement per kilogram of concentrate.By comparison with the steers fed the control concentrate supplement,those fed the concentrate containing the NPE deposited significantly more（P＜0.05）subcutaneous fat,intramuscular fat and tended to deposit more visceral fat.It was found that the steers fed the concentrate containing NPE displayed significantly greater（P＜0.05）expression of mRNA for heart fatty acid binding protein（H-FABP）in cardiac,loin muscle （Longissimus dorsi LD）and rump muscle（Biceps femoris,BF）tissue.In both groups of cattle the expression of mRNA for H-FABP in the tissues monitored was greatest in cardiac muscle and least in rump muscle with the differences between the tissues being significant（P＜0.05）.In the cattle fed the supplement containing NPE expression of mRNA for H-FABP was significantly greater in cardiac（P＜0.01）, loin（P＜0.0t）and rump（P＜0.05）muscle than in corresponding tissues from the steers fed the control supplement.

关键词： Natural plant extract Cattle fat deposition Heart fatty acid binding protein

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Expression of Lipid Metabolism-Associated Genes in Male and Female Chickens Selected for High Body Weight

Expression of Lipid Metabolism-Associated Genes in Male and ...

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中国畜牧兽医学会家禽学分会第九次代表会议暨第十六次全国...

作者： TU Yunjie P.B Siegel E.R.Gilbert SU Yijun ZHANG Xueyu LI Guohui YIN Jianmei WANG Kehua TONG Haibin Poultry Institute Chinese Academy of Agricultral Sciences Department of Animal and Poultry Sciences Virginia Polytechnic Institute and State University

1 Introduction The expression of many lipid-metabolic genes,including those involved in lipogenesis,holding and breaking down functional genes influences the rate of these processes and hence the tissue fat content.Genetic selection for boay weight and differential metabolic requirements of sexually-mature males and females may influence the regulation of these genes and hence feed intake regulation and body composition.In this study we determined

关键词： chicken fat deposition gene expression lipid metabolism

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