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Steroid hormone 20-hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae

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Insect Science 2023年第3期30卷 771-788页

作者： Sheng-Yu Zhang Han Gao Ankarjan Askar Xing-Peng Li Guo-Cai Zhang Tian-Zhong Jing Hang Zou Hao Guan Yun-He Zhao Chuan-Shan Zou School of Forestry Northeast Forestry UniversityHarbinChina Beiua University JilinChia

The steroid hormone 20-hydroxyecdysone(20E)has been described to regulate fat body lipid metabolism in insects,but its accurate regulatory mechanism,especially the crosstalk between 20E-induced lipid metabolism and gluconeogenesis remains largely ***,we specially investigated the effect of 20E on lipid metabolism and gluconeogenesis in the fat body of Hyphantria cunea larvae,a notorious pest in *** analysis showed that a total of 1907 lipid species were identified in the fat body of *** larvae assigned to 6 groups and 48 lipid *** differentially abundant lipids analysis showed a significant difference between 20E-treated and control samples,indicating that 20E caused a remarkable alteration of lipidomics profiles in the fat body of *** *** studies demonstrated that 20E accelerated fatty acidβ-oxidation,inhibited lipid synthesis,and promoted ***,the activities of pyruvate carboxylase,phosphoenolpyruvate carboxykinase,fructose-1,6-bisphosphatase,and glucose-6-phosphatase were dramatically suppressed by 20E in the fat body of *** *** well,the transcriptions of genes encoding these 4 rate-limiting gluconeogenic enzymes were significantly downregulated in the fat body of *** larvae after treatment with *** together,our results revealed that 20E disturbed fat body lipid homeostasis,accelerated fatty acidβ-oxidation and promoted lipolysis,but negatively regulated gluconeogenesis in *** *** findings might provide a new insight into hormonal regulation of glucose and lipid metabolism in insect fat body.

关键词： 20-hydroxyecdysone Hyphantria cunea fat body lipidomics analysis lipid metabolism gluconeogenesis

Clathrin-dependent endocytosis predominantly mediates protein absorption by fat body from the hemolymph in Bombyx mori

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Insect Science 2020年第4期27卷 675-686页

作者： Yu-Jie Wang Shu-Yan Li Jia-Ye Zhao Kang Li Jing Xu Xian-Ying Xu Wen-Mei Wu Rong Yang Yang Xiao Ming-Qiang Ye Ji-Ping Liu Yang-Jin Zhong Yang Cao Hui-Yu Yi Ling Tian Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding/Guangdong Provincial Sericulture and Mulberry Engineering Research Center College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences GuangzhouChina

During insect larval-pupal metamorphosis,proteins in the hemolymph are absorbed by the fat body for the maintenance of intracellular homeostasis;however,the type of proteins and how these proteins are internalized into the fat body are *** Bombyx mori,the developmental profiles o f total proteins in the hemolymph and fat body showed that hemolymph-decreased protein bands(55-100 kDa)were in accordance with those protein bands that increased in the fat *** o f clathrin-dependent endocytosis predominantly blocked the transportation o f 55-100 kDa proteins from the hemolymph into the fat body,which was further verified by RNA interference treatment o f *** hexamerins were shown to comprise〜90%of the total identified proteins in both the hemolymph and fat body by mass spectrum(MS)*** addition,hemolymphspecific proteins were mainly involved in material transportation,while fat body-specific proteins particularly participated in *** this paper,four hexamerins were found for the first time,and potential proteins absorbed by the fat body from the hemolymph through clathrin-dependent endocytosis were *** study sheds light on the protein absorption mechanism during insect metamorphosis.

关键词： Bombyx mori clathrin-mediated endocytosis fat body hemolymph uptake of proteins

Two dehydroecdysone reductases act as fat body-specific 20E catalyzers in Bombyx mori

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Insect Science 2022年第1期29卷 100-110页

作者： Lang You Zhiqian Li Zhongjie Zhang Bo Hu Ye Yu Fangying Yang Anjiang Tan Jiangsu Key Laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu Province212100China Key Laboratory of Silkworm and Mulberry Genetic Improvement Ministry of AgricultureThe Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangJiangsu Province212100China Key Laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai200032China

Periodic post-embryonic changes in insects, including growth, development and metamorphosis, are strictly controlled by many compounds, including steroid hormones. The biosynthesis and clearance of 20-hydroxyecdysone (20E), the major active form of the insect steroid hormone ecdysone, result in titer fluctuations that help control insect development. The inactivation of 20E in the silkworm Bombyx mori is highly tissue-specific, with CYP18A1 and ecdysone oxidase controlling 20E inactivation specifically in the mid-silk gland and midgut, respectively. Here, we characterized silkworm 3-dehydroecdysone 3α reductase (Bm3DE3α) and 3-dehydroecdysone 3β reductase (Bm3DE3β), two enzymes involved predominantly in the C-3-mediated catalysis of 20E in fat bodies. The ubiquitous and silk gland-specific overexpression of Bm3DE3α decreased the 20E titer, resulting in larval lethality and larval–pupal transition failure, respectively. In contrast, the ubiquitous and mid-silk gland-specific overexpression of Bm3DE3β increased the 20E titer, resulting in larval growth delays and lethality at the mid-fifth larval stage, respectively. Thus, Bm3DE3α and Bm3DE3β mediate fat body-specific steroid hormone metabolism in B. mori, indicating that highly diversified 20E metabolism-related mechanisms exist in different insect species.

关键词： Bm3DE3α Bm3DE3β Bombyx mori fat body 20-hydroxyecdysone

Differences in nutrient uptake between the fat body and embryonic primary cultures of silkworm （Bombyx mori）

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Insect Science 2006年第1期13卷 19-24页

作者： LEILA MATINDOOST JALAL J. SENDI HOORIEH SOLEIMAN JAHI KAYVAN ETEBARI Department of Plant Protection Faculty of Agriculture University of Guilan Rasht Department of Virology School of Medical Science Tarbiat Modarres Tehran Department of Sericulture Faculty of Natural Resources University of Guilan Somehesara Iran

Nutrition utilization and by-product formation in cultured insect cells has been investigated in several insect cells and has been of great interest to cell culturists and physiologists. In this research the biochemical changes in embryonic and fat body primary cultures of silkworm, Bombyx mori, have been compared. TC-100 medium supplemented with 10% and 20% FBS was used in embryonic and fat body primary cultures, respectively. Medium was renewed every week and the amount of glucose, uric acid, urea, total protein and alkaline phosphatase were measured in the samples from medium of primary cultures using spectrophotometeric methods. All biochemical macromolecules except uric acid showed significant changes. Glucose decreased in embryonic tissues, while in fat body culture its amount increased. Urea accumulation in embryonic culture was higher than in the fat body cultures. Since urea is a by-product, this accumulation could be due to higher utilization of amino acids. Total protein showed considerable changes and was consumed by embryonic culture more than the fat body＇ s. Alkaline phosphatase showed stronger activity in embryonic cells.

关键词： biochemical Bombyx mori embryo fat body insect cell primary culture,silkworm

Effect of Bmel-1/-2 mutation on the fat body metabolism in Bombyx mori

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Effect of Bmel-1/-2 mutation on the fat body metabolism in B...

The steroid-induced microRNA let-7 regulates developmental growth by targeting cdc7 in the Drosophila body

中国蚕学会2021年学术年会

作者： Baofeng HE Kaixiang ZhANG Hui YANG Sheng QIN Muwang LI Xia SUN Jiangsu Key Laboratory of Sericultural Biology and Biotechnology School of BiotechnologyJiangsu University of Science and Technology The Key Laboratory of Silkworm and Mulberry Genetic Improvement Ministry of AgricultureSericultural Research InstituteChinese Academy of Agricultural Science

fatty acid metabolism is mainly completed in the fat body,an important metabolic organ known as the "liver" in *** fat body is not only a place for the synthesis and storage of nutrients,but also an energy supply center to cope with the physiological changes and complete related metabolic functions during the development of the *** deficiency of BmEL-1/2 protein can cause defects in fatty acid metabolism of Bombyx *** figure out the influence of Bmel-1 and Bmel-2 genes on the metabolism of fat body,we analyzed the different metabolites of the fat body in Bmel-1-/Bmel-2-mutants compared with the normal individual *** data showed that there were a total of 54 differential metabolite in the Bmel-1-mutant and 93 differential metabolite in the *** of these differential metabolites are lipids and lipid-like molecules（37.50%/30.26%）,organic oxygen compounds（25%/22.37%）,Phenylpropanoids and polyketides（22.50%/21.05%）.By Kyoto Encyclopedia of Genes and Genomes analysis,most of these differential metabolic were significantly enriched in multiple amino acid metabolism *** with the inhibited growth and development phenotype of the Bmel-1-/Bmel-2-mutants,we speculated that in the Bmel-1/-2 mutant,the energy generation of fatty acid metabolism is inhibited,which induces the energy generation pathway of amino acid *** study reveals that Bmel-1/-2 genes could be involved in the energy metabolism of silkworm.

关键词： RNA binding protein development energy metabolism fat body silkworm

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Insect Science 2021年第6期28卷 1621-1632页

作者： Dan-Yan Huang Xiao-Ling Xia Run Huang Sheng Li Dong-Wei Yuan Su-Ning Liu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhou510631China

In insects,20-hydroxyecdysone(20E)limits systemic growth by triggering developmental *** studies have shown that 20E-induced let-7 exhibits crosstalk with the cell ***,we examined the underlying molecular mechanisms and physiological functions of 20E-induced let-7 in the fat body,an organ for energy storage and nutrient mobilization which plays a critical role in the larval ***,the overexpression of let-7 decreased the body size and led to the reduction of both nucleolus and cell sizes in the larval fat *** contrast,the overexpression of let-7-Sponge increased the nucleolus and cell ***,we found that cdc7,encoding a conserved protein kinase that controls the endocycle,is a target of ***,the mutation of cdc7 in the fat body resulted in growth ***,our findings revealed a novel role of let-7 in the control of endoreduplication-related growth during larval-prepupal transition in Drosophila.

关键词： cdc7 Drosophila fat body growth let-7

Transcriptomic analysis of epigenetic modification genes in the termite Reticulitermes speratus

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Insect Science 2020年第2期27卷 202-211页

作者： Yuki Mitaka Eisuke Tasaki Tomonari Nozaki Taro Fuchikawa Kazuya Kobayashi Kenji Matsuura Laboratory of Insect Ecology Graduate School of AgricultureKyoto UniversitySakyo-kuKyotoJapan Applied Entomology Laboratory Center for Bioresource Field ScienceKyoto Institute of TechnologyUkyo-kuKyotoJapan Laboratory of Animal Physiology Graduate School of ScienceOsaka City UniversitySumiyoshi-kuOsakaJapan Hokkaido Forest Research Station Field Science Education and Research CenterKyoto UniversityKawakami-gunHokkaidoJapan

Eusocial insects display a caste system in which different castes are morpho-logically and physiologically specialized for different *** studies have revealed that epigenetic modifications,including DNA methylation and histone modification,me-diate caste determination and differentiation,longevity,and polyethism in eusocial *** there has been a growing interest in the relationship between epigenetic mech-anisms and phenotypic plasticity in termites,there is ltte information about differential expression levels among castes and expression sites for these genes in *** we show royaltissuc-specific expression of epigenetic modification genes in the termite Reticulitermes *** RNA-seq,we identified 74 genes,including three DNA methyltransferases,seven sirtuins,48 Trithorax group proteins,and 16 Polycomb group *** these genes,15 showed king-specific expression,and 52 showed age-dependent differential expression in kings and *** real-time PCR revealed that DNA methyltransferase 3 is expressed specifically in the king's testis and fat body,whereas some histone modification genes are remarkably expressed in the king's testis and queen's *** findings imply that epigenetic modification plays important roles in the gamete production process in termite kings and queens.

关键词： aging division of labor fat body king ovary testis

The sex determination gene doublesex regulates expression and secretion of the basement membrane protein Collagen Ⅳ

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Journal of Genetics and Genomics 2022年第7期49卷 636-644页

作者： Qionglin Peng Jiangtao Chen Rong Wang Huan Zhu Caihong Han Xiaoxiao Ji Yufeng Pan The Key Laboratory of Developmental Genes and Human Disease School of Life Science and TechnologySoutheast UniversityNanjingJiangsu 210096China Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu 226019China

The highly conserved doublesex(dsx) and doublesex/mab-3 related(Dmrt) genes control sexually dimorphic traits across animals. The dsx gene encodes sex-specific transcription factors, Dsx^(M) in males and Dsx^(F) in females, which function differentially and often oppositely to establish sexual dimorphism. Here, we report that mutations in dsx, or overexpression of dsx, result in abnormal distribution of the basement membrane(BM) protein Collagen Ⅳ in the fat body. We find that Dsx isoforms regulate the expression of Collagen Ⅳ in the fat body and its secretion into the BM of other tissues. We identify the procollagen lysyl hydroxylase(dPlod) gene, which is involved in the biosynthesis of Collagen Ⅳ, as a direct target of Dsx. We further show that Dsx regulates Collagen Ⅳ through d Plod-dependent and independent pathways. These findings reveal how Dsx isoforms function in the secretory fat body to regulate Collagen Ⅳ and remotely establish sexual dimorphism.

关键词： Drosophila Sexual dimorphism doublesex Dmrt genes CollagenⅣ dPlod fat body

Hexamerin and allergen are required for female reproduction in the American cockroach,Periplaneta americana

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Insect Science 2024年第1期31卷 186-200页

作者： Shiming Zhu Xiaoyi Chen Sishi Xia Qin Li Ziqi Ye Shaoting Zhao Kexin Liu Fangfang Liu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologyInstitute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouChina

Reproduction is of great importance for the continuation of the *** insects,the fat body is the major tissue for nutrient storage and involved in vitellogenesis,which is essential for female ***,2 proteins,hexamerin and allergen,were separated from the fat bodies of adult female American cockroaches(Periplaneta americana)and identified as storage proteins,encoding for 733 amino acids with molecular weight of 87.88 kDa and 686 amino acids with molecular weight of 82.18 kDa,*** encoding genes of these 2 storage proteins are mainly expressed in the fat *** interference-mediated knockdown of Hexamerin and Allergen in the early stage of the first reproductive cycle in females suppressed vitellogenesis and ovarian maturation,indicating that these storage proteins are involved in controlling ***,the expression of Hexamerin and Allergen was repressed by knockdown of the juvenile hormone(JH)receptor gene Met and the primary response gene Kr-h1,and was induced by methoprene,a JH analog,in both in vivo and in vitro ***,we have determined that hexamerin and allergen are identified as storage proteins and play an important role in promoting female reproduction in the American *** expression of their encoding genes is induced by JH *** data reveal a novel mechanism by which hexamerin and allergen are necessary for JH-stimulated female reproduction.

关键词： fat body juvenile hormone ovary reproduction storage protein vitellogenesis