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falcaria vulgaris extract attenuates diabetes-induced kidney injury in rats

Asian Pacific Journal of Tropical Biomedicine 2019年第4期9卷 150-157页

作者： Jalili Cyrus Roshankhah Shiva Salahshoor Mohammad Reza Medical Biology Research Center Kermanshah University of Medical Sciences Department of Anatomical Sciences Medical School Kermanshah University of Medical Sciences

Objective: To assess the effects of falcaria vulgaris(F. vulgaris) as an antioxidant on damage to kidney of diabetic rats. Methods: Diabetic rats were established via streptozotocin(60 mg/kg). Various doses of F. vulgaris extracts(50, 100 and 150 mg/kg) and streptozotocin + F. vulgaris extracts were administered via intraperitoneal(i.p) injection to 48 rats(n=8 per group) for 28 d. Subsequently, ferric ion reducing antioxidant power(FRAP) of renal tissue, thiobarbituric acid reactive species, blood glucose concentrations, insulin, nitrite oxide, the weight of animals, glomeruli characteristics and kidney function were evaluated. Results: Compared with the control rats, diabetic rats showed significant increase in malondialdehyde, blood urea nitrogen, creatinine, blood glucose, nitrite oxide contents in renal tissues, and glomerular diameter. Furthermore, tissue FRAP level, body weight, number of glomeruli and plasma insulin were markedly reduced in diabetic rats when compared with the control group(P < 0.05). However, in all F. vulgaris and F. vulgaris + streptozotocin groups, malondialdehyde level, blood urea nitrogen, creatinine, glomerular diameter, nitrite oxide, and glucose levels were decreased significantly; meanwhile, tissue FRAP level, body weight, glomeruli number and insulin serum level were increased, compared to the control diabetic group(P < 0.05). Conclusions: F. vulgaris extract alleviates renal damage in diabetic rats.

关键词： Antioxidant Diabetes falcaria vulgaris Kidney

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Characterization of Nuclear and Chloroplast Microsatellite Markers for <i>falcaria vulgaris</i>(Apiaceae)

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American Journal of Plant Sciences 2013年第3期4卷 590-595页

作者： Sarbottam Piya Madhav P. Nepal Department of Biology and Microbiology South Dakota State University Brookings SD USA.

falcaria vulgaris (sickleweed) is native to Eurasia and a potential invasive plant of the United States. No molecular markers have been developed so far for sickleweed. Characterization of molecular markers for this plant would allow investigation into its population structure and biogeography thereby yielding insights into risk analysis and effective management practices of the plant. In order to characterize the molecular markers, DNA samples were collected from eight populations in Iowa, Nebraska and South Dakota. Nuclear microsatellite markers developed for other Apiaceae taxa were screened and tested for intergeneric transferability to sickleweed. The chloroplast trnL intron and trnL-F intergenic spacer regions were sequenced and the sequences were used to design primers to amplify the microsatellites present within each region. We characterized eight polymorphic microsatellite markers for sickleweed that included six nuclear and two chloroplast markers. Our result showed intergeneric transferability of six nuclear microsatellite markers from Daucus carota to F. vulgaris. The markers we characterized are useful for population genetic study of F. vulgaris.

关键词： falcaria vulgaris Invasive Species Microsatellite trnL Intron trnL-trnF Intergenic Spacer Sickleweed

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