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EFFECT OF HEPATIC STIMULATOR SUBSTANCE (HSS) EXTRACTED FROM fetal liver ON THE PROLIFERATION OF HUMAN ALEXENDER HEPATOMA CELLS

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Chinese Journal of Cancer Research 1991年第4期3卷 55-58页

作者：单英张振生熊诗松买凯 Hepatitis Division Henan Institute of Medical Sciences Henan Medical University Zhengzhou

It's reported that hepatic stimulator substance (HSS) was extracte from the fetal liver of 4 - 6 months of fetus, and that the effect of HSS on the proliferation of human Alexender hepatoma cells was studied in this paper. The results showed that proliferation of Alexender cells varied with the amount of HSS in the culture medium, and the former was positively correlated with the latter significantly (Pfetal liver can stimulate the proliferation of human Alexender hepatoma cells.

关键词： HSS fetal liver hepatoma cell line.

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fetal liver Transplantation in the Treatment of β-Thalassemia Major:Report of Two Cases

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Military Medical Research 1987年第3期 249-253页

作者：宋静澄何杏周芷芬张肇和 Guangzhou Department of Pediatrics First Military Medical College

Two cases of β-thalassemia major treated with fetal liver transplanta-tion(FLT)were reported.A conditioning regimen of total body irradiation pluscyclophosphamide was given,followed by the infusion of suspensions of fetal *** number of nucleated cells(procared from 3 to 4 fetal livers)infused tothe 2 cases was 17.5 and 15.5×10~8/kg *** hemopoieticdepression was observed after FLT,and normal hemopoiesis was recoveredaround the 30th *** analysis revealed that the donor typechromosomes was founed in the peripheral blood of both cases from the 20th dayto 123rd day after *** biopsy of cass 1 revealed changes of mild GVHD,indicating engrafment of fetal hemopoietic cells and formation of chimeras in *** hemopoiesis was reconstituted in both cases two months *** transfusion was not necessary within the first 5 months after FLT,and in case 1 transfusion was given 3 times only within 21 months after FLT.

关键词： fetus fetal liver transplantation β-thalassemia major

Transplantation of fetal liver epithelial progenitor cells ameliorates experimental liver fibrosis in mice

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World Journal of Gastroenterology 2006年第45期12卷 7292-7298页

作者： Jin-Fang Zheng Li-Jian Liang Chang-Xiong Wu Jin-Song Chen Zhen-Sheng Zhang Department of Hepatobiliary Surgery the First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Department of Hepatobiliary Surgery the People's Hospital of Hainan Province Haikou 570311 Hainan Province China

AIM： To investigate the effect of transplanted fetal liver epithelial progenitor （FLEP） cells on liver fibrosis in mice. METHODS： FLEP cells were isolated from embryonal day （ED） 14 BALB/c mice and transplanted into female syngenic BALB/c mice （n = 60）. After partial hepatectomy （PH）, diethylnitrosamine （DEN） was administered to induce liver fibrosis. Controls received FLEP cells and non-supplemented drinking water, the model group received DEN-spiked water, and the experimental group received FLEP cells and DEN. Mice were killed after 1, 2, and 3 mo, and alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, hyaluronic acid （HA）, and laminin （LN） in serum, and hydroxyproline （Hyp） content in liver were assessed. Alpha-smooth muscle actin （α-SMA） of liver was tested by immunohistochemistry. Transplanted male mice FLEP cells were identified by immunocytochemistry for sty （sex determination region for Y chromosome） protein. RESULTS： Serum ALT, AST, HA, and LN were markedly reduced by transplanted FLEP cells. liver Hyp content and （α-SMA staining in mice receiving FLEP cells were lower than that of the model group, which was consistent with altered liver pathology. Transplanted cells proliferated and differentiated into hepatocytes and bile duct epithelial cells with 30%-50% repopulation in the liver fibrosis induced by DEN after 3 mo. CONCLUSION： Transplanted FLEP cells proliferate and differentiate into hepatocytes and bile duct epithelial cells with high repopulation capacity in the fiberized liver induced by DEN, which restores liver function and reduces liver fibrosis.

关键词： fetal liver Epithelial progenitor cells Ce therapy Proliferation liver fibrosis

Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker

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World Journal of Gastroenterology 2007年第16期13卷 2319-2323页

作者： Parveen Nyamath Ayesha Alvi Aejaz Habeeb Sanjeev Khosla Aleem A Khan CM Habibullah Center for liver Research and Diagnostics Deccan College of Medical Sciences and Allied Hospitals Kanchanbagh Hyderabad-58 Andhra Pradesh India Centre for DNA Fingerprinting and Diagnostics Nacharam Hyderabad 500072 India

AIM: To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. METHODS: Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation. RESULTS: Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture. CONCLUSION: The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations.

关键词： Progenitor cells fetal liver Magnetic cell sorting Flow cytometry Immunohistochemistry

Promoted differentiation of cynomolgus monkey ES cells into hepatocyte-like cells by co-culture with mouse fetal liver-derived cells

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World Journal of Gastroenterology 2006年第42期12卷 6818-6827页

作者： Ko Saito Masahide Yoshikawa Yukiteru Ouji Kei Moriya Mariko Nishiofuku Shigehiko Ueda Noriko Hayashi Shigeaki Ishizaka Hiroshi Fukui Department of Gastroenterology and Hepatology Nara Medical University Kashihara Nara Japan Division of Developmental Biology Department of Parasitology Nara Medical University Kashihara Nara Japan

AIM: To explore whether a co-culture of cynomolgus monkey embryonic stem (cES) cells with embryonic liver cells could promote their differentiation into hepatocytes. METHODS: Mouse fetal liver-derived cells (MFLCs) were prepared as adherent cells from mouse embryos on embryonic d (ED) 14, after which undifferentiated cES cells were co-cultured with MFLCs. The induction of cES cells along a hepatic lineage was examined in MFLC- assisted differentiation, spontaneous differentiation, and growth factors (GF) and chemicals-induced differentiations (GF-induced differentiation) using retinoic acid, leukemia inhibitory factor (LIF), FGF2, FGF4, hepatocyte growth factor (HGF), oncostatin M (OSM), and dexamethasone. RESULTS: The mRNA expression of α-fetoprotein, albumin (ALB), α-1-antitrypsin, and hepatocyte nuclear factor 4α was observed earlier in the differentiating cES cells co-cultured with MFLCs, as compared to cES cells undergoing spontaneous differentiation and those subjected to GF-induced differentiation. The expression of cytochrome P450 7a1, a possible marker for embryonic endoderm-derived mature hepatocytes, was only observed in cES cells that had differentiated in a co-culture with MFLCs. Further, the disappearance of Oct3/4, a representative marker of an undifferentiated state, was noted in cells co-cultured with MFLCs, but not in those undergoing spontaneous or GF-induced differentiation. Immunocytochemical analysis revealed an increased ratio of ALB-immunopositive cells among cES cells co-cultured with MFLCs, while glycogen storageand urea synthesis were also demonstrated. CONCLUSION: MFLCs showed an ability to induce cES cells to differentiate toward hepatocytes. The co-culture system with MFLCs is a useful method for induction of hepatocyte-like cells from undifferentiated cES cells.

关键词： Primate embryonic stem cells fetal liver Hepatic differentiation Co-culture

Disruption of palladin leads to defects in definitive erythropoiesis by interfering erythroblastic island formation in mouse fetal liver

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Disruption of palladin leads to defects in definitive erythr...

Small non-coding RNAs transfer through mammalian placenta and directly regulate fetal gene expression

中国遗传学会“发育、遗传和疾病”研讨会

作者： Xue-Song Liu~(1,4) Xi-Hua Li~1 Yi Wang~1 Run-Zhe Shu~(1,4) Long Wang~(1-3) Shun-Yuan Lu~(1-3) Hui Kong~3 Yue-E Jin~1 Li-Jun Zhang~1 Jian Fei~3 Sai-Juan Chen~2 Zhu Chen~2 Ming-Min Gu~1 Zhen-Yu Lu~1 Zhu-Gang Wang~(1-3,*) 1 Laboratory of Genetic Engineering,Institute of Health Sciences,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences & Shanghai Jiao Tong University School of Medicine,Shanghai 200025,PR China 2 State Key Laboratory of Medical Genomics,Rui-jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200025,PR China 3 Shanghai Research Center for Model Organisms,Shanghai 201203,PR China 4 Graduate School of Chinese Academy of Sciences,Shanghai 200025,PR China.

Palladin was originally found up-regulated with NB4 cell differentiation induced by *** of palladin results in neural tube closure defects,liver herniation and embryonic *** we further report thatpailadinembryos exhibit a significant defect in erythropoiesis,characterized by dramatic reduction in definitive erythrocytes derived from fetal liver but not primitive erythrocytes from yolk *** reduction of erythrocytes is accompanied by increased apoptosis of erythroblasts and partial blockage of erythroid ***,colony forming assay shows no differences between wt and mutant fetal liver or yolk sac in the number and size of colonies *** addition,palladinfetal liver cells can reconstitute hematopoiesis in lethally-irradiated *** data strongly suggest that deficient erythropoiesis in palladin fetal liver is mainly due to compromised erythropoietic *** expected,erythroblastic island in palladin fetal liver was found *** liver cells fail to form erythroblastic island in ***,wt macrophages can form such units with either wt or mutant erythroblasts,while mutant macrophages lose their ability to bind wt or mutant *** data demonstrate that palladin is crucial for definitive erythropoiesis and erythroblastic island formation,and especially,required for normal function ofmacrophages in fetal liver.

关键词： Palladin fetal liver Erythropoiesis Microenvironment Macrophage.

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Protein & Cell 2015年第6期6卷 391-396页

作者： Jing Li Yujing Zhang Dameng Li Yuchen Liu Danping Chu Xiaohong Jiang Dongxia Hou Ke Zen Chen-Yu zhang State Key Laboratory of Pharmaceutical Biotechnology Nanjing Advanced Institute for Life Sciences (NAILS) Nanjing University School of Life Sciences Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnoiogy Nanjing University 22 Hankou Road Nanjing 210093 Jiangsu China

SMALL NON-CODING RNA CAN TRANSFER THROUGH THE PLACENTA AND REGULATE fetal DEVELOPMENT Increasing evidence demonstrated that exogenous plant miRNAs in the diet can be absorbed and are found in mammalian tissues. These miRNAs influence the physio- logical and pathological progression of the recipient. In this issue, Jing Li and colleagues further reveal that exogenous/ endogenous small non-coding RNA in the maternal system can transfer through the placenta to the fetal side and influ- ence fetal development and health. These surprising finding demonstrate that maternal miRNAs profiling are very impor- tant to fetal development, which means maternal diet and the healthy status may influence fetal development and the postnatal morbidity via transplacental small non-coding RNAs. Furthermore, this finding also proposes a brand new potential strategy to treat fetal diseases in utero. Combining with gene therapy, maternal administrating represents a new trend for treating fetal diseases.

关键词： Amniotic Fluid fetal liver fetal Development Maternal Plasma Rubella Virus

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人胎肝组织块腹腔输注治疗重症型肝炎(附九例报告)

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中华器官移植杂志 1989年第4期10卷 164-165页

作者：吕新生韩明翁绍纪胡国龄湖南医科大学第一附属医院器官移植研究室湖南医科大学第一附属医院传染病学教研室

本文报道采用胎肝组织块腹腔内注射治疗9例重症型肝炎, 除1例死亡外, 其余8例均康复出院。胎肝细胞于注射后3～7天开始发挥作用, 治疗后37 ～103天临宋症状及黄疸消退, 肝功能恢复。认为腹腔是一较理想的移植部位。

关键词：人胎肝组织块腹腔输注治疗重症肝炎 Treatment fetal liver

不同胎龄的胎肝及胎髓粒单系祖细胞及OKT4/OKT8比值的研究

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中华器官移植杂志 1992年第3期13卷 101-102页

作者：洪平张肇和中国人民解放军第157医院三内科第一军医大学附属南方医院小儿科

采用体外琼脂半固体培养法观察22例胎龄为11～31周的胎肝及胎髓粒单系祖细胞(CFU-GM)产率的动态变化。胎髓于妊娠20周以前曾有过CFU-GM产率高峰, 20周降低, 以后多次出现CFU-GM产率高峰。胎肝CFU-GM的产率于妊娠17～20周时达高峰, 以后... 详细信息

采用体外琼脂半固体培养法观察22例胎龄为11～31周的胎肝及胎髓粒单系祖细胞(CFU-GM)产率的动态变化。胎髓于妊娠20周以前曾有过CFU-GM产率高峰, 20周降低, 以后多次出现CFU-GM产率高峰。胎肝CFU-GM的产率于妊娠17～20周时达高峰, 以后迅速下降, 于25周时降至极低水平。24周前胎肝、胎髓的OKT4/OKT8比值均小于1.3。认为OKT4/OKT8的比值超过1.3时, 移植术后发生移植物抗宿主病的机率增高;胎髓造血干细胞移植较胎肝造血干细胞移植的存活率高, 但须采取预防严重移植物抗宿主病发生的措施。

关键词：胎龄胎肝粒单系祖细胞比值 Investigation CFU-GM Bone Marrow fetal liver