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Construction and Functional Analysis of CRISPR/Cas9 Vector of fad2 gene family in Soybean

Phyton-International Journal of Experimental Botany 2022年第2期91卷 349-361页

作者： Zhifeng Xiao Yukun Jin Qi Zhang Abraham Lamboro Baozhu Dong Zhuoyu Yang Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun130118China

Soybean oleic acid content is one of the important indexes to evaluate the quality of soybean *** the synthesis pathway of soybean fatty acids,the fad2 gene family is the key gene that regulates the production of linoleic acid from soybean oleic *** this study,CRISPR/Cas9 gene editing technology was used to regulate fad2 gene ***,the CRISPR/Cas9 single knockout vectors Gmfad2-1B and Gmfad2-2C and double knockout vectors Gmfad2-2A-3 were ***,the three vectors were transferred into the recipient soybean variety Jinong 38 by Agrobacterium-mediated cotyledon node transformation,and the mutant plants were *** analysis and comparison of the mutant plants of the T2 and T3 generations were carried *** results showed that there was no significant difference in agronomic traits between the CRISPR/Cas9 single and double knockout vectors and the untransformed CRISPR/Cas9 receptor *** oleic acid content of the plants that knocked out the CRISPR/Cas9 double gene vector was significantly higher than that of the single gene vector.

关键词： Soybean fad2 gene family CRISPR/Cas9 oleic acid content

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Expression proteomic and transcriptome analysis of peanut seeds in the different developmental stages revealed candidate genes for oil accumulation pathways

Expression proteomic and transcriptome analysis of peanut se...

引用

2019年中国作物学会学术年会

作者：殷冬梅 Yun Wang Xingguo Zhang Xingli Ma Zhongfeng Li Henan Agricultural University

Peanuts are one of the most important edible oil crops in the world. In order to survey key genes controlling peanut oil accumulation, we analyzed the seed transcriptome and proteomic analysis in different developmental stages of high-and low-oil peanut varieties. Over 1,500 genes involved in lipid metabolism were identified, classified, and found to participate in FA synthesis and TAG assembly. There were seven possible metabolic pathways involved in the accumulation of oil during seed development. Proteomic analyses were carried out 20, 40, 60 and 80 days after pollination, and 5, 10, 20 and 30 days after germination to determine the protein profiles of lipid dynamics during peanut seed development and post germination. A total of 5,712 of 8,505 proteins were identified, quantified and divided into 23 functional groups, the largest of which was metabolism-related. Further analyses of the proteins and their pathways revealed initiation of fatty acid accumulation at early stages after flowering, while lipid degradation occurred largely through the lipoxygenase-dependent pathway. Further study was investigated on molecular genetic mechanism of high oleic acid peanut traits, In this study, six ahfad2 genes were cloned and analyzed, using the CRISPR-CAS9 site-directed mutagenesis system constructed of ahfad2 gene to further investigate the function of ahfad2 and screen the genetically materials of different oleic acid content for further cultivating the high oleic acid peanut varieties.

关键词： peanut lipid metabolism oil body proteomic fad2 gene family genetic transformation CRISPR-CAS9 system

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