检索结果-南通市图书馆

Detection of beta-lactamase and extended-spectrum beta-lactamase of Pathogens Isolated from Pig and Chicken and Their Antibiotic Susceptibility Test

引用

Agricultural Sciences in China 2005年第11期4卷 877-882页

作者： HU Gong-zheng ZHANG Chun-hui YUAN Li YANG Yu-rong LIANG Jun Faculty of Animal Husbandary and Veterinary Science Henan Agricultural University Zhengzhou 450002 P.R.China Zhengzhou College of Animal Husbandary Engineering Zhengzhou 450006 P.R.China

The antibacterial activity of beta-lactam antibiotics or their combinations with inhibitor sulbactum against non-lactamase- producing strains, lactamase-producing and ESBLs-producing isolates was evaluated with twofold dilution method after pathogens isolated from pigs and chickens were detected, respectively, for beta-lactamase and extended-spectrum beta- lactamases （ESBLs）, The results revealed that most of 43 clinically isolated strains could produce beta-lactamase and 3 strains of shigella isolated from chicken samples produced ESBLs. All of 30 lactamase-producing strains isolated and only one of 16 non-lactamase-producing strains were resistant to amoxicillin and ampicillin. MICs of ampicillin against lactamaseproducing isolates decreased 10-40 and 10-20 times respectively, when it was conbined with sulbactam at ration of 1：2 and 1：4. All clinical isolates were susceptible to third-generation cephalosporins. The MICs of third-generation cephalosporins against lactamase-producing isolates did not change when they were conbined with sulbactam. MICs of ceftiofur and ceftriaxone against ESBLs-producing isolates decreased 2-4 times when they were conbined with sulbactam.

关键词： beta-lactamase extended-spectrum beta-lactamase Inhibitor Antibiotic susceptibility test

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes

引用

Chinese Medical Journal 2014年第17期127卷 3051-3057页

作者： Xu Li Zhai Yao Lyu Yuan Wang Qi An Shuchang Chen Jichao Chen Yusheng Liu Lin Li Jiabin Gao Zhancheng Department of Respiratory and Critical Care Medicine Peking University People's Hospital Beijing 100044 China Institute of Clinical Pharmacology Peking University First Hospital Peking University Beijing 100034 China Department of Respiratory Medicine the Second Affiliated Hospital to Dalian Medical University Dalian Liaoning 116023 China Department of Respiratory Medicine First Hospital of Tsinghua University Beijing 100016 China Department of-Respiratory Medicine the Central Hospital of China Aerospace Corporation Beijing 100049 China Department of Respiratory Medicine Fujian Provincial Hospital Fuzhou Fujian 350001 China Department of Infectious Diseases First Affiliated Hospital of Anhui Medical University Hefei Anhui 230022 China

Background The extended-spectrum beta-lactamase （ESBL）-producing Klebsiella pneumoniae has increasingly become a major contributor to nosocomial infections and can exhibit multiple antibiotic *** studies have focused on the resistance genes in ESBL-producing strains,and the resistance-associated genetic environment of non-ESBL-producing strains has been ignored until ***,we investigated the occurrence and characteristics of non-ESBL-producing ***,which potentially carries unexpressed resistance *** *** strains were collected from five medical institutions in China from February 2010 to August *** VITEK-2 ESBL detection system was used as a primary screen to identify the ESBL-producing phenotype,and the three primary types of ESBL-associated genes （CTX,SHV,and TEM） were detected by polymerase chain reaction （PCR） to confirm the strains presenting with a non-ESBL-producing *** expression in the non-ESBL-producing strains was further screened by reverse-transcription PCR （RT-PCR） to validate their transcriptional *** Out of 224 clinically isolated antibiotic-sensitive *** strains with a non-ESBL-producing phenotype,5 （2.2％） were identified to carry inactivated ESBL blaSHV genes with intact upstream promoter regions and resistance gene ***,three of the five antibiotic-sensitive *** strains containing ESBL blaSHV genes still exhibited mRNA transcription of blasHv,while the other two exhibited no mRNA *** These findings suggest that inactivated ESBL genes exist in non-ESBL-producing antibiotic-sensitive *** strains,which have the potential to transform the strain into an ESBL phenotype if an inappropriate application or overdose of antibiotics is implemented during clinical management.

关键词： Klebsiella pneumoniae extended-spectrum beta-lactamase gene silencing beta-lactam resistance promoter regions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Zoonotic sources and the spread of antimicrobial resistance from the perspective of low and middle-income countries

引用

Infectious Diseases of Poverty 2023年第3期12卷 73-87页

作者： Ioana D.Olaru Birgit Walther Frieder Schaumburg Institute of Medical Microbiology University of MünsterMünsterGermany Advanced Light and Electron Microscopy Robert Koch-InstituteBerlinGermany Department of Environmental Hygiene German Environment AgencyBerlinGermany

Background Antimicrobial resistance is an increasing challenge in low and middle-income countries as it is wide-spread in these countries and is linked to an increased *** from human and environmental factors,animal-related drivers of antimicrobial resistance in low-and middle-income countries have special features that differ from high-income *** aim of this narrative review is to address the zoonotic sources and the spread of antimicrobial resistance from the perspective of low-and middle-income *** body Contamination with extended-spectrum beta-lactamase(ESBL)-producingEscherichia coli is highest in poultry(Africa:8.9–60%,Asia:53–93%)and there is a risk to import *** through poultry meat in *** aquacultures,the proportion of ESBL-producers *** can be high(27%)but the overall low quality of published studies limit the general conclusion on the impact of aquacultures on human *** colonization of wildlife is 1–9%in bats or 2.5–63%*** most of them are migratory animals,they can disperse antimicrobial resistant bacteria over large ***-called‘filth flies’are a relevant vector not only of enteric pathogens but also of antimicrobial resistant bacteria in settings where sanitary systems are *** Africa,up to 72.5%of‘filth flies’are colonized with ***,mostly conferred by CTX-M(24.4–100%).While methicillin-resistantStaphylococcus aureus plays a minor role in livestock in Africa,it is frequently found in South America in poultry(27%)or pork(37.5–56.5%)but less common in Asia(poultry:3%,pork:1–16%).Conclusions Interventions to contain the spread of AMR should be tailored to the needs of low-and middle-income *** comprise capacity building of diagnostic facilities,surveillance,infection prevention and control in small-scale farming.

关键词： Antimicrobial resistance extended-spectrum beta-lactamase Methicillin-resistant Staphylococcus aureus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：