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expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs

Journal of Animal Science and Biotechnology 2017年第1期8卷 30-42页

作者： Xiao Chen Jinluan Fu Aiguo Wang College of Animal Sciences and Technology National Engineering Laboratory for Animal Breeding & Key Laboratory of Animal Genetics Breeding and Reproduction Ministry of Agriculture China Agricultural University Beijing 100193 People's Republic of China Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing 100093 People's Republic of China

Background： Embryonic mortality during the period of implantation strongly affects litter size in pigs. Progesterone receptor（PGR） paracrine signaling has been recognized to play a significant role in embryonic implantation. IHH,NR2F2, BMP2, FKBP4 and HAND2 were proved to involve in PGR paracrine signaling. The objective of this study was to evaluate the expression of IHH, NR2F2, BMP2, FKBP4 and HAND2 in endometrium of pregnant sows and to further investigate these genes＇ effect on litter size in pigs. Real-time PCR, western blot and immunostaining were used to study target genes/proteins expression in endometrium in pigs. RFLP-PCR was used to detect single nucleotide polymorphisms（snps） of target ***： The results showed that the m RNA and protein expression levels of IHH, NR2F2 and BMP2 were up-regulated during implantation period（P 〈 0.05 or P 〈 0.01）. All target proteins were mainly observed in luminal epithelium and glandular epithelium. Interestingly, the staining of NR2F2 and HAND2 was also strong in stroma. snps detection revealed that there was a-204 C 〉 A mutation in promoter region of NR2F2 gene. Three genotypes were found in Large White, Landrace and Duroc sows. A total of 1847 litter records from 625 sows genotyped at NR2F2 gene were used to analyze the total number born（TNB） and number born alive（NBA）. The study of the effect on litter size suggested that sows with genotype CC tend to have higher litter ***： These results showed the expression patterns of genes/proteins involved in PGR paracrine signaling over implantation time. And the candidate gene for litter size was identified from genes involved in this signaling. This study could be a resource for further studies to identify the roles of these genes for embryonic implantation in pigs.

关键词： expression implantation litter size pigs snps

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