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An Improved Method of Simultaneous Determination of Four Bioactive Compounds in evodiae fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

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Chemical Research in Chinese Universities 2017年第4期33卷 552-558页

作者： BIAN Min TIAN Ling YAO Cheng College of Chemistry and Molecular Engineering Nanj'ing Tech University Nanjing 211800 P. R. China

There are many compounds with different structures and chemical properties in evodiae fructus. It is hard to simultaneously determine the bioactive compounds by high performance liquid chromatography（HPLC）. A new method was proposed for four bioactive compounds（synephrine, limonoids, evodiamine and rutecarpine） to be separated completely and determined accurately using ionic liquids（ILs） as mobile phase additives. The mechanism and the effect of the ILs for changing the chromatographic behaviors of the four compounds were studied by systemati- cally changing the pH value of mobile phase, the types and concentrations of ILs as well as the concentrations of phosphate buffer. The chromatographic behaviors of the analytes with a mobile phase containing ILs complied with the stoichiometric displacement model for retention（SDM-R）. All results demonstrate the dual nature of ionic liquids, which are competitive adsorption and ion-pair agent. Meanwhile, excellent linearity was observed for all the com- pounds with correlation coefficients between 0.9992 and 0.9998. The limit of detection and the limit of quantification of the four compounds varied from 0.47μg/mL to 0.87 μg/mL and from 1.79 μg/mL to 2.44μg/mL, respectively. Three kinds of evodiae fructus processed through different methods were analyzed via the method. The result shows that the contents of evodiamine and rutecarpine as the two main active compounds by processing with vinegar and salt are obviously higher than those of the raw products.

关键词： evodiae fructus Ionic liquid Mobile phase additive High performance liquid chromatography(HPLC) Processed evodiae fructus

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Simultaneous analytical method of the bioactive marker compounds of a herbal drug,evodiaefructus

Simultaneous analytical method of the bioactive marker compo...

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30th International Symposium on Capillary Chromatography、4th GC×GC Symposium、16th National Symposium and Exhibition on Chromatography,i.e. 1st Dalian International Symposium and Exhibition on Chromatography

作者： Moon,D.C. Kim,N.H. Jeong,E.S. Cho,H.E. Ahn,S.Y. Hwang,B.Y. Lee,Y.M. Lee,J.J. College of Pharmacy CBITRC Chungbuk NationalUniversityCheongju 361-763Korea Korea Research Institute of Bioscience and Biotechnology Daejon 305-600 Korea

HPLC-PDA and LC-tandem MS methods have been developed and validated for the simultaneous analyses of indolequinazoline-(IQAs)and quinolone alkaloids(QAs)in a herbal drug,“evodiae fructus”.The bioactive marker compounds,evodiamine(1),rutaecarpine(2)and quinolone alkaloids were simultaneously determined in the 20 drug specimens that collected from Korea and China markets..HPLC analysis was performed on a reversed-phase phenyl-C6 column using solvent system of MeOH(A):20mM NH4OAc(B):AcCN(C)using gradient flow detecting at UV ***-ESI-MS/MS analysis was accomplished by multiple reaction monitoring(MRM)method after separation on a minibore phenyl-C6 column using 74% AcCN(5mM NHAc). Contents of evodiamine(1)and rutaecarpine(2)were in the range between 0.12-1.14% and 0.07-1.02%, respectively,which together with other compounds,showed variable profiles depending on specimens. Clustering analysis was performed as an evaluating mean of the herbal *** methods would be useful as a crucial quality control of the herbal drug or the related products.

关键词： evodiae fructus Marker compounds HPLC-PDA LC-ESI-MS/MS

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