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Effects of euphorbiasteroid on gene expression in lung cancer cells based on gene chip

TMR Modern Herbal Medicine 2022年第3期5卷 48-58页

作者： Zi-Ye Yang Ming-Rui Jiang Xiao-Tong Wei Zhi-Cheng Wang Zhu-Zhu Yue Jing-Qiu Zhang Meng-Yu Chen Hui-Nan Wang Meng-Lin Wang Shuang-Hui Shi Ying-Zi Wang School of Chinese Materia Medica Beijing University of Chinese Medicine102488BeijingChina

Objective Using gene chip technology to explore the molecular mechanism of euphorbiasteroid in the treatment of non-small cell lung cancer(NSCLC).Methods A549 cells were used as the in vitro model,and they were randomly divided into control group and different concentrations of euphorbiasteroid administration *** group had 3 duplicate wells,after the cells were cultured in vitro,the cell viability was evaluated by CCK-8 *** chip technology was used to screen the differentially expressed genes(DEGs)between the control group and the euphorbiasteroid administration *** differential genes were further analyzed for Gene Ontology(GO)function enrichment and Kyoto Encyclopedia of Genes and Genome(KEGG)pathway enrichment *** STRING online analysis platform combined with Cytoscape software to construct a target protein interaction(PPI)network and perform topological analysis to screen key targets,and use Real-time PCR(RT-PCR)and molecular docking technology to verify key *** According to the analysis of gene chip data,276 differentially expressed genes were screened,including 117 up-regulated genes and 159 down-regulated *** analysis showed that differentially expressed genes were mainly involved in cell division,cell proliferation,cell cycle and other processes,involving protein binding,protein kinase binding and other functions,and were mainly distributed in nucleoplasm,chromosomes and other *** signaling pathway analysis showed that differentially expressed genes were involved in cell cycle,pyrimidine metabolism,p53 signaling pathway and other *** network analysis showed that CCNA2,TOP2A,CCNB1,CDC20,and RRM2 may be the key targets of euphorbiasteroid in the treatment of ***-PCR results showed that the expressions of CCNA2,TOP2A,CCNB1,CDC20,and RRM2 were significantly down-regulated in the euphorbiasteroid administered group,which was consistent with the gene chip *** docking results showed t

关键词： euphorbiasteroid non-Small cell lung cancer Gene chip Differentially expressed genes Molecular docking

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