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ANALYSIS OF EVOLUTIONARY RELATIONSHIP BETWEEN ASTASIA LONGA AND euglena gracilis BY USING RAPD TECHNIQUE AND CLADISTIC ANALYSIS

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Chinese Journal of Oceanology and Limnology 2001年第1期19卷 40-50页

作者：王江新施之新甘小妮谢树莲中科院水生生物所湖北武汉 430072

Although both Astasia longa and euglena gracilis belong to different genera, they share many morphological characters except that A. longa has no chloroplast. In the 1940’s, on the basis of the finding that in darkness or upon addition of some chemicals, E. gracilis would fade reversibly or irreversibly, some scholars hypothesised that A. longa evolved from E. gracilis by losing chloroplast. The authors’ use of RAPD and cladistic analyses in a study on the evolutionary relationship between A .longa and E. gracilis showed that the A. longa ’s relationship with E. gracilis was closer than that with other green euglenoids. This proves the hypothesis that A. longa evolved from E. gracilis is reasonable. The results of this study suggest that saprophytic colorless euglenoids were transformed from green euglenoids by losing their choroplasts.

关键词： A. longa euglena gracilis cladistic analysis molecular evolution RAPD

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Residual plastids of bleached mutants of euglena gracilis and their effects on the expression of nucleus-encoded genes

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Progress in Natural Science:Materials International 2004年第3期 21-25页

作者： WANG Jiangxin, SHI Zhixin and XU Xudong(Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China) InstituteofHydrobiology ChineseAcademyofSciencesWuhan430072China

Bleached mutants of euglena gracilis were obtained by treatment with ofloxacin (Ofl)and streptomycin (Sm) respectively. As shown by electron microscopy, the residual plastids contain prothylakoids in an Ofl mutant, and the highly developed and tightly stacked membranous structure found in cells of two Sm mutants. Nine genes of the plastid genome were examined with PCR, showing that ribosomal protein genes and most other plastid genes were lost in all but one Sm mutant. Using differential display and RT PCR, it was shown that chloroplast degeneration could cause changes in transcription of certain nucleus encoded genes during heterotrophic growth in darkness.

关键词： euglena gracilis bleached mutant residual plastids tightly stacked membranous structure nucleus encoded genes regulation.

温度对纤细裸藻（euglena gracilis）脂肪酸组成的影响

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温度对纤细裸藻（Euglena gracilis）脂肪酸组成的影响

γ-Irradiation Effect:Variation of Photosynthetic Activity of euglena

2015年中国水产学会学术年会

作者：崔钟国金锋姜昌旼崔炳大姜石中国立庆尚大学海洋食品科学与水产养殖学院

在15℃-35℃条件下,于恒温培养箱中兼养培养纤细裸藻（euglena gracilis）3天,分别采集纤细裸藻样本,采用毛细管气相色谱法测定脂肪酸组成和含量,研究了纤细裸藻在不温度条件中的总脂含量和脂肪酸组成的变化。实验结果表明纤细裸藻在25... 详细信息

在15℃-35℃条件下,于恒温培养箱中兼养培养纤细裸藻（euglena gracilis）3天,分别采集纤细裸藻样本,采用毛细管气相色谱法测定脂肪酸组成和含量,研究了纤细裸藻在不温度条件中的总脂含量和脂肪酸组成的变化。实验结果表明纤细裸藻在25℃条件下的粗脂肪含量（占鲜质量17.13%）明显高于其他组（15.79-9.48%）。二十二碳六烯酸在15℃,20℃,25℃,30℃及35℃条件下含量分别为4.11%,4.33%,0.52%,0.51%和0.59%,n-3高度不饱和脂肪酸为24.88%,25.20%,9.49%,12.96%和10.37%。.因此,根据纤细裸藻的用途可通过设置不同培养温度来调控脂肪酸组成。该研究阐明培养温与脂肪酸组成的变化关系,为纤细裸藻的应用奠定基础。

关键词： euglena gracilis 温度兼养培养脂肪酸 DHA

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Biomedical and Environmental Sciences 2002年第3期15卷 261-267页

作者： T·SAKASHITA M·DOI 等 NationalInstituteofRadinologicalSciences

Objective To study the effects of gamma-ray irradiation on carbon fixation (Specific production rate: SPR), CO2 utilization efficiency (CUE) and electron transfer rate (ETR) in the photosynthetic flagellate euglena gracilis strain Z in a dose-response dependent manner. Methods euglena cells were cultured in an inorganic nutrient medium containing ammonium chloride or proteose peptone. Cells were exposed to gamma-ray at 5 doses (0, 100, 250, 350, 500 Gy for water). Five days after irradiation, three photosynthetic activities were measured. SPR, which is a carbon uptake rate per unit carbon mass, was determined by 13C tracer methodology. CUE was evaluated using a relation of carbon isotope fractionation in Calvin cycle. ETR in photosystem II (PS II) was measured by a chlorophyll fluorescence analysis. Results Even at a dose of 500 Gy, 80 % of ETR of the non-irradiated control (0 Gy) was sustained, while SPR and CUE were about half the level in the non-irradiated control at 500 Gy. Furthermore, the dose response of ETR was considerably different from the others. Conclusion Our findings suggest that not only PS II but also the Calvin cycle in the photosynthetic system is affected by gamma ray irradiation.

关键词： Gamma-ray irradiation euglena gracilis Photosynthetic activities

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