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The Ca^(2+)-dependent DNases are Involved in Secondary Xylem Development in eucommia ulmoides

Journal of Integrative Plant Biology 2012年第7期54卷 456-470页

作者： Hui-Min Chen Yu Pang Jun Zeng Qi Ding Shen-Yi Yin Chao Liu Meng-Zhu Lu Ke-Ming Cui Xin-Qiang He State KeyLaboratory of Protein and Plant Gene Research College of Life SciencesPeking UniversityBeijing 100871China Laboratory of Biotechnology Research Institute of ForestryChinese Academy of ForestryBeijing 100091China

Secondary xylem development has long been recognized as a typical case of programmed cell death （PCD） in plants. During PCD, the degradation of genomic DNA is catalyzed by endonucleases. However, to date, no endonuclease has been shown to participate in secondary xylem development. Two novel Ca^2＋-dependent DNase genes, EuCaN1 and EuCaN2, were identified from the differentiating secondary xylem of the tree eucommia ulmoides.Oliv., their functions were studied by DNase activity assay, in situ hybridization, protein immunolocalization and virus-induced gene silencing experiments. Full-length cDNAs of EuCaN1 and EuCaN2 contained an open reading frame of 987 bp, encoding two proteins of 328 amino acids with SNase-like functional domains. The genomic DNA sequence for EuCaN1 had no introns, while EuCaN2 had 8 introns. EuCaN1 and EuCaN2 digested ssDNA and dsDNA with Ca^2＋-dependence at neutral pH. Their expression was confined to differentiating secondary xylem cells and the proteins were localized in the nucleus. Their activity dynamics was closely correlated with secondary xylem development. Secondary xylem cell differentiation is influenced by RNAi of endonuclease genes. The results provide evidence that the Ca^2＋-dependent DNases are involved in secondary xylem development.

关键词： Ca^2+-dependent DNase programmed cell death secondary xylem development secondary wall formation eucommia ulmoides.

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