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Phosphorylation of an ethylene response factor by MPK3/MPK6 mediates negative feedback regulation of pathogen-induced ethylene biosynthesis in Arabidopsis

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Journal of Genetics and Genomics 2022年第8期49卷 810-822页

作者： Xiaoyang Wang Huicong Meng Yuxi Tang Yashi Zhang Yunxia He Jinggeng Zhou Xiangzong Meng Shanghai Key Laboratory of Plant Molecular Sciences College of Life SciencesShanghai Normal UniversityShanghai 200233China

Plants under pathogen attack produce high levels of the gaseous phytohormone ethylene to induce plant defense responses via the ethylene signaling *** 1-aminocyclopropane-1-carboxylate synthase(ACS)is a critical rate-limiting enzyme of ethylene *** and post-translational upregulation of ACS2 and ACS6 by the mitogen-activated protein kinases MPK3 and MPK6 are previously shown to be crucial for pathogen-induced ethylene biosynthesis in ***,we report that the fungal pathogen Botrytis cinerea-induced ethylene biosynthesis in Arabidopsis is under the negative feedback regulation by ethylene signaling *** ethylene response factor ERF1 A is further found to act downstream of ethylene signaling to negatively regulate the ***-induced ethylene biosynthesis via indirectly suppressing the expression of ACS2 and ***,ERF1 A is shown to also upregulate defensin genes directly and therefore promote Arabidopsis resistance to ***,ERF1 A is identified to be a substrate of MPK3 and MPK6,which phosphoactivate ERF1 A to enhance its functions in suppressing ethylene biosynthesis and inducing defensin gene *** together,our data reveal that ERF1 A and its phosphorylation by MPK3/MPK6 not only mediate the negativefeedback regulation of the ***-induced ethylene biosynthesis,but also upregulate defensin gene expression to increase Arabidopsis resistance to ***.

关键词： ethylene response factor Mitogen-activated protein kinase Protein phosphorylation ethylene biosynthesis Defensin gene induction Disease resistance

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Crystal structure and molecular evolution of AtACS7, the key enzyme for senescence ethylene biosynthesis in Arabidopsis

Crystal structure and molecular evolution of AtACS7, the key...

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第三届全国植物开花·衰老与采后生物学大会

作者： Yuanyuan Mei Bowei Hao Gongling Sun Chang Xu Xin Li Ning Ning Wang Tianjin Key Laboratory of Protein Sciences College of Life Sciences Nankai University

ethylene is a widely acknowledged positive regulator of leaf senescence. The rate-limiting enzyme that controls ethylene synthesis in higher plants is ACC synthase（ACS）. We evaluated the production of ethylene and revealed an up-regulation of AtACS7 during leaf senescence in Arabidopsis. High-level accumulation of AtACS7 protein promotes leaf senescence while the acs7-1 mutant exhibits delayed natural senescence. In addition, the N-terminal 14 residue-mediated degradation of AtACS7 is highly regulated by senescence signaling to enable plants to produce appropriate levels of ethylene required. It was further found that AtACS7 protein possesses ACS and C-S lyase dual enzyme activities while the ACS-like protein in early diverging bryophyte Physcomitrella patens only encodes a C-S lyase. Investigations using multidisciplinary methods, such as biochemistry and molecular biology, structural biology and bioinformatics, suggest that ACSs in seed plants may originate from C-S lyase, not aspartate aminotransferases as generally believed. We also found that several key motifs may play important roles during the molecular evolution of ACS proteins.

关键词： ethylene biosynthesis AtACS7 leaf senescence crystal structure molecular evolution

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Abscisic Acid Antagonizes ethylene Production through the ABI4-Mediated Transcriptional Repression of ACS4 and ACS8 in Arabidopsis

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Molecular Plant 2016年第1期9卷 126-135页

作者： Zhijun Dong Yanwen Yu Shenghui Li Juan Wang Saijun Tang Rongfeng Huang College of Biological Sciences China Agricultural University Beijing 100193 China plant Molecular Biology Department Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China National Key Facility of Crop Gene Resources and Genetic Improvement Beijing 100081 China These authors contributed equally to this article

Increasing evidence has revealed that abscisic acid （ABA） negatively modulates ethylene biosynthesis, although the underlying mechanism remains unclear. To identify the factors involved, we conducted a screen for ABA-insensitive mutants with altered ethylene production in Arabidopsis. A dominant allele of ABI4, abi4-152, which produces a putative protein with a 16-amino-acid truncation at the C-terminus of ABI4, reduces ethylene production. By contrast, two recessive knockout alleles of ABI4, abi4-102 and abi4-103, result in increased ethylene evolution, indicating that ABI4 negatively regulates ethylene produc- tion. Further analyses showed that expression of the ethylene biosynthesis genes ACS4, ACSS, and AC02 was significantly decreased in abi4-152 but increased in the knockout mutants, with partial dependence on ABA. Chromatin immunoprecipitation-quantitative PCR assays showed that ABI4 directly binds the pro- moters of these ethylene biosynthesis genes and that ABA enhances this interaction. A fusion protein containing the truncated ABI4-152 peptide accumulated to higher levels than its full-length counterpart in transgenic plants, suggesting that ABI4 is destabilized by its C terminus. Therefore, our results demon- strate that ABA negatively regulates ethylene production through ABI4-mediated transcriptional repression of the ethylene biosynthesis genes ACS4 and ACS8 in Arabidopsis.

关键词： ABA ABI4 ethylene biosynthesis stress response transcriptional regulation

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Dual and opposing roles of EIN3 reveal a generation conflict during seed growth

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Molecular Plant 2022年第2期15卷 363-371页

作者： Juliane Heydlauff Isil Erbasol Serbes Dieu Vo Yanbo Mao Sonja Gieseking Thomas Nakel Theresa Harten Ronny Völz Anja Hoffmann Rita GroΒ-Hardt University of Bremen Centre for Biomolecular Interactions Bremen(CBIB)Leobenerstrasse 528359 BremenGermany ZMBP University of TübingenAuf der Morgenstelle 3272076 TübingenGermany

Seed size critically affects grain yield of crops and hence represents a key breeding *** develop-ment of embryo-nourishing endosperm is a key driver of seed *** here report unexpected dual roles of the transcription factor EIN3 in regulating seed *** EIN3 functions have remained largely undiscovered because they oppose each *** on the analysis of multiple ethylene biosynthesis mutants,we demonstrate that EIN3 represses endosperm and seed development in a pathway regulated by ***,in addition,provide evidence that EIN3-mediated synergid nucleus disintegration promotes endosperm ***,synergid nucleus disintegration is not affected in various ethylene biosynthesis mutants,suggesting that this promoting function of EIN3 is inde-pendent of *** the growth-inhibitory ethylene-dependent EIN3 action appears to be encoded by sporophytic tissue,the growth-promoting role of EIN3 is induced by fertilization,revealing a generation conflict that converges toward the key signaling component EIN3.

关键词： seed size EIN3 ethylene biosynthesis fertilization generation conflict synergid disintegration

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Molecular mechanisms of remorin1 to regulate fruit ripening and resistance

Molecular mechanisms of remorin1 to regulate fruit ripening ...

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中国植物学会第十六次全国会员代表大会暨85周年学术年会

作者： Jianghua Cai Guozheng Qin Tong Qin Shiping Tian Key Laboratory of Plant Resources Institute of Botanythe Chinese Academy of Sciences University of Chinese Academy of Sciences

Remorins are plant-specific and plasma membrane-associated proteins that display a variety of functions in plant growth,development,biotic and abiotic stresses and signal ***,little information is available for understanding their role in fruit ripening and ***,we investigate SlREM1 expression during tomato fruit development and ripening,and determine its localization in plasma *** find that overexpression of SlREM1 in tomato can promote fruit ripening with higher lycopene accumulation and ethylene production,and enhance fruit resistance to Botrytis cinerea causing grey mold rot in various *** further prove SlREM1 function,that regulates fruit ripening via mediating expression of key genes related to ethylene and lycopene *** on co-immunoprecipitation coupled with mass spectroscopy and luciferase complementation imaging assays,we ascertain that SlREM1 can interact with ethylene biosynthesis protein SlSAM1 to regulate ethylene,and be degraded by ubiquitin-mediated *** findings suggest that SlREM1 positively regulates fruit ripening via affecting ethylene and lycopene biosynthesis,and affects tomato fruit resistance against fungal pathogen.

关键词： Remorin fruit ripening resistance ethylene biosynthesis lycopene accumulation

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