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Effect of epigallocatechin gallate in green tea on preventing lens opacity and αB-crystallin aggregation in rat model of diabetes

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International Journal of Ophthalmology(English edition) 2023年第3期16卷 342-347页

作者： Andita Gustria Caesary Nina Handayani Hidayat Sujuti Department of Ophthalmology Faculty of MedicineUniversitas BrawijayaDr.Saiful Anwar General HospitalMalang 65111Indonesia

AIM:To evaluate the effect of epigallocatechin gallate(EGCG) in preventing lens opacity and the aggregation of lens αB-crystallin in model rats of diabetes mellitus(DM).METHODS:This experimental study included Wistar rats for DM as in vivo models and divided into 5 groups.The treatment groups were administered EGCG by orally for 20d and were then assessed for their degree of lens opacity with binocular microscope and lens αB-crystallin expression from Western blot analyze.RESULTS:Pearson correlation test and regression analysis on EGCG exposure and final random blood sugar(RBS) obtained a significance level of P<0.05.EGCG exposure can significantly lower RBS with an R~2 of 0.5634(56.34%).The same analysis on EGCG exposure and the degree of lens opacity obtained a significance level of P<0.05 and increased exposure to EGCG can significantly lower the degree of lens opacity with an R~2 of 0.8577(85.77%).Correlation analysis between EGCG and the expression of lens αB-crystallin can be concluded that the higher the EGCG exposure administered,the higher the native lens αB-crystallin expression and the lower the aggregate lens αB-crystallin expression.There was also significant effect in which every 1 mg/kg body weight dose of EGCG can increase the native lens αB-crystallin expression by 0.0063 and decrease the aggregate lens αB-crystallin expression by 0.0076.CONCLUSION:The administration of EGCG at a dose of 300,600,and 1200 mg shows a significant effect on preventing lens opacity and aggregation of αB-crystallin in diabetic rat models and this research could be a biomolecular prevention of cataract.

关键词： diabetes mellitus epigallocatechin gallate cataract lensαB-crystallin

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Green tea, epigallocatechin gallate and the prevention of Alzheimer’s disease: clinical evidence

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Food Science and Human Wellness 2022年第4期11卷 765-770页

作者： Klaus W.Lange Katharina M.Lange Yukiko Nakamura Department of Experimental Psychology University of RegensburgRegensburg 93040Germany

Given its increasing global prevalence,Alzheimer’s disease(AD)has become a major public health challenge worldwide.The symptomatic treatments available for AD have shown no significant efficacy,and no disease-modifying interventions are capable of slowing the progression of the disorder.The potential of lifestyle-related factors,including diet,is increasingly recognized as an important consideration in the primary prevention of AD.Numerous mechanisms potentially underlying neuroprotective effects of bioactive components contained in tea,such as(-)-epigallocatechin-3-gallate,as well as their preventive efficacy against AD,have been elucidated in preclinical studies.However,in contrast to the abundance of mechanistic findings in animals,clinical results demonstrating efficacy in humans are scarce.While epidemiological studies have provided some evidence indicating that green tea consumption is associated with a reduced risk of age-related cognitive decline and AD,a causal relationship cannot be established on the basis of these observations.The clinical evidence regarding preventive or therapeutic effects of green tea and its bioactive components is unsatisfactory.A role of green tea in the prevention of AD cannot be recommended until well-designed,randomized,placebo-controlled clinical trials using standardized formulations confirm the purported beneficial effects of green tea.

关键词： Green tea epigallocatechin gallate Alzheimer’s disease Neurodegeneration Prevention

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Therapeutic effects of epigallocatechin and epigallocatechin gallate on the allergic reaction ofα_(s1)-casein sensitized mice

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Food Science and Human Wellness 2023年第3期12卷 882-888页

作者： Qianqian Zhang Xiaofeng Yu Linghan Tian Yanjun Cong Linfeng Li Beijing Advanced Innovation Center for Food Nutrition and Human Health College of Food and HealthBeijing Technology and Business UniversityBeijing 100048China Department of Dermatology Beijing Friendship HospitalBeijing 100050China

To investigate the anti-α_(s1)-casein allergy mechanism of two tea-derived polyphenols,epigallocatechin(EGC)and epigallocatechin gallate(EGCG),BALB/c mice were sensitized and challenged withα_(s1)-casein and nutritional intervention was given by EGC and EGCG during the sensitization provocation phase.The main evaluation indexes used were levels of mast cell proteases,histamine,and specific antibody immunoglobulin E(IgE),as well as cytokine secretion and pathological observation.The results showed that both EGC and EGCG significantly reduced levels of mast cell protease,histamine,specific IgE antibodies,and Th2 cytokines in allergic mice.The histopathology results showed that both EGC and EGCG markedly reduced the degree of lesions in the intestine,thymus,spleen,and lung.The conclusions from this study can provide a theoretical basis for the mechanism by which tea polyphenols regulate food allergens.

关键词： α_(s1)-casein epigallocatechin epigallocatechin gallate Antianaphylaxis

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Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury？

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Neural Regeneration Research 2018年第1期13卷 119-127页

作者： Jiri Ruzicka Lucia Machova Urdzikova Barbora Svobodova Anubhav G. Amin Kristyna Karova Jana Dubisova Kristyna Zaviskova Sarka Kubinova Meic Schmidt Meena Jhanwar-Uniyal Pavla Jendelova Institute of Experimental Medicine Academy of Sciences of the Czech Republic Videfiska Prague Czech Republic Department of Neuroscience Charles University Second Faculty of Medicine Prague Czech Republic New York Medical College Valhalla NY USA

Systematic inflammatory response after spinal cord injury （SCI） is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate （EGCG） are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin ＋ EGCG or saline [daily intraperitoneal doses （curcumin, 6 mg/kg; EGCG 17 mg/kg）] and weekly intramuscular doses （curcumin,60 mg/kg; EGCG 17 mg/kg）] for 28 days. Rats were evaluated using behavioral tests （the Basso, Beattie, and Bresnahan （BBB） open-field locomotor test, flat beam test）. Spinal cord tissue was analyzed using histological methods （Luxol Blue-cresyl violet staining） and mmunohistochemistry （anti-glial fibrillary acidic protein, anti-growth associated protein 43）. Cytokine levels （interleukin-1β, interleukin-4, interleukin-2,interleukin-6, macrophage inflammatory protein 1-alpha, and RANTES） were measured using Luminex assay. Quantitative polymerase chain reaction was performed to determine the relative expression of genes （Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, NfκB, Cntf） related to regenerative processes in injured spinal cord. We found that all treatments displayed significant behavioral recovery, with no obvious synergistic effect after combined therapy of curcumin and ECGC. Curcumin and EGCG alone or in combination increased axonal sprouting, decreased glial scar formation, and altered the levels of macrophage inflammatory protein 1-alpha, interleukin-1β, interleukin-4 and interleukin-6 cytokines. These results imply that although the expected synergistic response of this combined therapy was less obvious, aspects of tissue regeneration and immune responses in severe SCI were evident.

关键词： spinal cord injury epigallocatechin gallate curcumin inflammatory response neural regeneration

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Structural shift of gut microbiota during chemopreventive effects of epigallocatechin gallate on colorectal carcinogenesis in mice

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World Journal of Gastroenterology 2017年第46期23卷 8128-8139页

作者： Xin Wang Tao Ye Wen-Jie Chen You Lv Zong Hao Jun Chen Jia-Ying Zhao Hui-Peng Wang Yuan-Kun Cai Department of General Surgery The 5 People’s Hospital of ShanghaiFudan University

AIM To investigate the effect of epigallocatechin gallate(EGCG) on structural changes of gut microbiota in colorectal carcinogenesis.METHODS An azoxymethane(AOM)/dextran sodium sulfate(DSS)-induced colitis mouse model was established. Fortytwo female FVB/N mice were randomly divided into the following three groups: group 1(10 mice, negative control) was treated with vehicle, group 2(16 mice, positive control) was treated with AOM plus vehicle, and group 3(16 mice, EG) was treated with AOM plus EGCG. For aberrant crypt foci(ACF) evaluation, the colons were rapidly took out after sacrifice, rinsed with saline, opened longitudinally, laid flat on a polystyrene board, and fixed with 10% buffered formaldehyde solution before being stained with 0.2% methylene blue in saline. For tumor evaluation, the colon was macroscopically inspected and photographed, then the total number of tumors was enumerated and tumor size measured. For histological examination, the fixed tissues were paraffin-embedded and sectioned at 5 mm thickness. Microbial genomic DNA was extracted from fecal and intestinal content samples using a commercial kit. The V4 hypervariable regions of 16 S r RNA were PCR-amplified with the barcoded fusion primers. Using the best hit classification option, the sequences from each sample were aligned to the RDP 16 S r RNA training set to classify the taxonomic abundance in QIIME. Statistical analyses were then performed.RESULTS Treatment of mice with 1% EGCG caused a significant decrease in the mean number of ACF per mouse, when compared with the model mice treated with AOM/DSS(5.38 ± 4.24 vs 13.13 ± 3.02, P < 0.01). Compared with the positive control group, 1% EGCG treatment dependently decreased tumor load per mouse by 85%(33.96 ± 6.10 vs 2.96 ± 2.86, respectively, P < 0.01). All revealed that EGCG could inhibit colon carcinogenesis by decreasing the number of precancerous lesions as well as solid tumors, with reduced tumor load and delayed histological progression of CRC. During the cancerization, the diversity

关键词： epigallocatechin gallate Gut microbiota Colorectal cancer High throughput sequencing Chemoprevention Animal experiment

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Comparison of the impact of epigallocatechin gallate and ellagic acid in an experimental cataract model induced by sodium selenite

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International Journal of Ophthalmology(English edition) 2017年第4期10卷 499-506页

作者： Irfan Ergen Burak Turgut Nevin Ilhan Diskapi YildirimBeyazit Training and Research Hospital Eye Diseases Clinic Ankara 06330 Turkey Department of Ophthalmology Faculty of Medicine Firat University Elazig 23119 Turkey Department of Biochemistry Faculty of Medicine Firat University Elazig 23119 Turkey

AIM：To compare the potential protective effects of epigallocatechin gallate（EGCG） and ellagic acid（EA） in an experimental cataract model.●METHODS：Twenty-eight Spraque-Dawley rat pups were assigned into four groups.All the rats,except for those in the control group,were injected subcutaneously sodium selenite to induce experimental cataract on the postpartum ninth day,and between 10 th and 14 th days.Rats in the sham,EGCG,and EA groups were intraperitoneally administered 50 mg/（kg·d） saline solution,50 mg/（kg·d） EGCG and 200 mg/（kg·d） EA,respectively.The reduced glutathione（GSH） and malondialdehyde（MDA） levels,total antioxidant status（TAS） and total oxidant status（TOS） in lens supernatants were measured.RESULTS：The mean cataract gradings in EGCG and EA groups were found to be significantly lower than that in sham group（P〈0.001）.The mean GSH levels and TASs in EGCG and EA groups were significantly higher than that in sham group while mean MDA levels and TOSs in EGCG and EA groups were significantly lower than that in the sham group（P〈0.001）.CONCLUSION：EGCG and EA have protective effects on cataract development via the inhibition of oxidative stress.

关键词： sodium selenite experimental cataract epigallocatechin gallate ellagic acid total oxidant status totalanti-oxidant status.

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Study on the epigallocatechin gallate and Konjac Glucomannan Mosaic Topological Structure

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Chinese Journal of Structural Chemistry 2017年第9期36卷 1447-1455页

作者：洪馨倪永升林婉媚穆若郡王林庞杰吴春华温成荣 College of Food Science Fujian Agriculture and Forestry University School of Food Science and Technology National Engineering Research Center of SeafoodDalian Polytechnic University

In order to effectively protect the activity of epigallocatechin gallate（EGCG）, we explored the protection mechanism of Konjac glucomannan（KGM） for EGCG by experiments and theory analyses. We synthesized KGM/EGCG nanofibers by using electrostatic spinning method. The microstructure of nanofibers was characterized by SEM, FTIR, TGA, XRD and Raman spectroscopic. The formation mechanism and the protection effects of KGM/EGCG nanofibers were also discussed. The results showed that the EGCG activity was protected due to the hydrogen bonds between-OH of EGCG and KGM, and EGCG was embedded in KGM nanofiber with bead style. The reducing force and DPPH scavenging ability data indicated that KGM/EGCG nanofibers have stronger antioxidant activity than the EGCG solution under the same condition. Hence, the mosaic topological structure of KGM can effectively extend the EGCG activity.

关键词： KGM epigallocatechin gallate nanofiber mosaic topology activity protection

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Targeting fatty acid synthase sensitizes human nasopharyngeal carcinoma cells to radiationvia downregulating frizzled class receptor 10

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Cancer Biology & Medicine 2020年第3期17卷 740-752页

作者： Jiongyu Chen Fan Zhang Xiaosha Ren Yahui Wang Wenhe Huang Jianting Zhang Yukun Cui Guangdong Provincial Key Laboratory for Breast Cancer Diagnosis and Treatment Cancer Hospital of Shantou University Medical CollegeShantou 515041China Department of Cancer Biology University of Toledo College of Medicine and Life SciencesToledoOH 43614USA

Objective:Our aim was to test the hypothesis that fatty acid synthase(FASN)expression contributes to radioresistance of nasopharyngeal carcinoma(NPC)cells and that inhibiting FASN enhances radiosensitivity.Methods:Targeting FASN using epigallocatechin gallate(EGCG)or RNA interference in NPC cell lines that overexpress endogenous FASN was performed to determine their effects on cellular response to radiationin vitro using MTT and colony formation assays,andin vivo using xenograft animal models.Western blot,immunohistochemistry,real-time PCR arrays,and real-time RT-PCR were used to determine the relationship between FASN and frizzled class receptor 10(FZD10)expression.FZD10 knockdown and overexpression were used to determine its role in mediating FASN function in cellular response to radiation.Immunohistochemical staining was used to determine FASN and FZD10 expressions in human NPC tissues,followed by analysis of their association with the overall survival of patients.Results:FASN knockdown or inhibition significantly enhanced radiosensitivity of NPC cells,bothin vitro andin vivo.There was a positive association between FASN and FZD10 expression in NPC cell lines grown as monolayers or xenografts,as well as human tissues.FASN knockdown reduced FZD10 expression,and rescue of FZD10 expression abolished FASN knockdown-induced enhancement of radiosensitivity.FASN and FZD10 were both negatively associated with overall survival of NPC patients.Conclusions:FASN contributes to radioresistance,possiblyvia FZD10 in NPC cells.Both FZD10 and FASN expressions were associated with poor outcomes of NPC patients.EGCG may sensitize radioresistance by inhibiting FASN and may possibly be developed as a radiosensitizer for better treatment of NPCs.

关键词： epigallocatechin gallate fatty acid synthase frizzled class receptor 10 nasopharyngeal carcinoma radioresistance

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EGCG inhibits cardiomyocyte apoptosis in pressure overload-induced cardiac hypertrophy and protects cardiomyocytes from oxidative stress in rats

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Acta Pharmacologica Sinica 2007年第2期28卷 191-201页

作者： Rui SHENG~(2,3,4) Zhen-Iun GU~(2,3,4) Mei-lin XIE Wen-xuan ZHOU~3 Ci-yi GUO~32 Department of Pharmacology,Medical School of Soochow University Suzhou 215123 3 Suzhou Institute of Chinese Materia Medica,Suzhou 215007 China Department of Pharmacology Medical School of Soochow University Suzhou China Suzhou Institute of Chinese Materia Medica Suzhou China

Aim:To investigate the effects of epigallocatechin gallate（EGCG）on pressureoverload and hydrogen peroxide（H2O2）induced cardiac myocyte apoptosis.Methods:Cardiac hypertrophy was established in rats by abdominal aorticconstriction.EGCG 25,50 and 100 mg/kg were administered intragastrically（ig）.Cultured newborn rat cardiomyocytes were preincubated with EGCG,and oxida-tive stress injury was induced by H2O2.Results:In cardiac hypertrophy inducedby AC in rats,relative to the model group,EGCG 25,50 and 100 mg/kg ig for 6weeks dose-dependently reduced systolic blood pressure（SBP）and heart weightindices,decreased malondialdehyde（MDA）content,and increased superoxidedismutase（SOD）and glutathione peroxidase（GSH-PX）activity,both in serumand in the myocardium.Also,treatment with EGCG 50 and 100 mg/kg markedlyimproved cardiac structure and inhibited fibrosis in HE and van Gieson（VG）stain,and reduced apoptotic myocytes in the hypertrophic myocardium detected byterminal transferase-mediated dUTP-biotin nick end-labeling（TUNEL）assay.Inthe Western blot analysis,EGCG significantly inhibited pressure overload-inducedp53 increase and bcl-2 decrease.In H2O2-induced cardiomyocyte injury,whenpreincubated with myocytes for 6-48 h,EGCG 12.5-200 mg/L increased cell viabil-ity determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay.EGCG also attenuated H2O2-induced lactate dehydrogenase（LDH）release and MDA formation,Meanwhile,EGCG 50 and 100 mg/L significantlyinhibited the cardiomyocyte apoptotic rate in flow cytometry.Conclusion:EGCGinhibits cardiac myocyte apoptosis and oxidative stress in pressure overload in-duced cardiac hypertrophy.Also,EGCG prevented cardiomyocyte apoptosisfrom oxidative stress in vitro.The mechanism might be related to the inhibitoryeffects of EGCG on p53 induction and bcl-2 decrease.

关键词： epigallocatechin gallate hypertrophy apoptosis oxidative stress cardiac myocytes

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EGCG抑制PRRSV增殖的分子机制

EGCG抑制PRRSV增殖的分子机制

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作者：于朋伟河南农业大学

学位级别：硕士

猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）属于动脉炎病毒科,具有囊膜的单股正链RNA病毒。PRRSV感染导致母猪妊娠后期流产、公猪精液品质下降、仔猪呼吸系统疾病和死亡。PRRSV是危害全球... 详细信息

猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus,PRRSV）属于动脉炎病毒科,具有囊膜的单股正链RNA病毒。PRRSV感染导致母猪妊娠后期流产、公猪精液品质下降、仔猪呼吸系统疾病和死亡。PRRSV是危害全球养猪业的重要病原体之一,给全球养猪业带来了巨大的经济损失。表没食子儿茶素没食子酸酯（epigallocatechin gallate,EGCG）是绿茶中的一种多酚类化合物,EGCG是儿茶素中含量最多的活性物质,对多种病毒具有抗病毒活性。本研究深入研究了EGCG抑制PRRSV增殖的分子机制,为PRRSV的防控提供了新的策略。主要研究结果如下:1.EGCG限制PRRSV的复制和组装本研究首先通过CCK-8法检测EGCG对细胞活力、细胞周期和凋亡的影响。研究结果表明EGCG（0-10μM）对猪肺泡巨噬细胞和Marc-145细胞的细胞活力、细胞周期进程和凋亡均无显著影响。病毒滴度测定结果表明EGCG均可抑制高致病性和低致病性PRRSV在猪肺泡巨噬细胞和Marc-145细胞中的复制。为了确定EGCG如何抑制PRRSV复制,检测了EGCG对PRRSV生活周期的影响,结果表明EGCG抑制PRRSV的复制和组装,但不影响病毒的附着、进入和释放。2.EGCG抑制PRRSV诱导的脂滴形成和脂质合成本研究接下来检测了EGCG是否影响PRRSV诱导的脂滴形成和脂质合成。油红O和BODIPY染色结果表明PRRSV感染显著增加猪肺泡巨噬细胞和Marc-145细胞中脂滴数量,而EGCG抑制这一现象。qRT-PCR和Western blotting结果表明,EGCG抑制PRRSV诱导的脂质合成相关基因（SREBF1、FASN、ACACA、ACACB、SCD、SREBF2、HMGCR）mRNA和蛋白的表达;进一步检测了细胞内的脂质含量,发现PRRSV增加细胞内胆固醇、甘油三酯和游离脂肪酸的含量,而EGCG使胆固醇、甘油三酯和游离脂肪酸的含量回复到对照细胞水平。以上结果表明EGCG抑制PRRSV诱导的脂滴形成和脂质合成。3.EGCG抑制PRRSV诱导的脂噬本研究最后检测了EGCG是否影响PRRSV诱导的脂噬。Western blotting和免疫荧光结果表明EGCG抑制PRRSV诱导的LC3-Ⅱ表达、P62降解和自噬流的发生。EGCG处理细胞并外源补加油酸后,能够回复PRRSV的复制和组装,进而回复PRRSV增殖。以上结果表明EGCG通过抑制细胞内脂质代谢阻碍PRRSV增殖。本研究发现EGCG处理能够使PRRSV导致的脂滴堆积消失,随后本研究通过EGCG处理感染细胞后脂质合成以及自噬水平的变化,阐明了EGCG抑制PRRSV增殖的分子机制,为临床病毒防控提供了新的思路。

关键词：猪繁殖和呼吸综合征病毒 epigallocatechin gallate 脂质合成自噬

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