检索结果-南通市图书馆

Behavioral Effects of Deep Brain Stimulation of the Anterior Nucleus of Thalamus, Entorhinal Cortex and Fornix in a Rat Model of Alzheimer＇s Disease

引用

Chinese Medical Journal 2015年第9期128卷 1190-1195页

作者： Chao Zhang Wen-Han Hu De-Long Wu Kai Zhang Jian-Guo Zhang Department of Neurosurgery Beijing Tian Tan Hospital Capital Medical University Beijing 100050 China Beijing Key Laboratory of Neurostimulation Beijing 100050 China Stereotactic and Functional Neurosurgery Laboratory Beijing Neurosurgical Institute Capital Medical University Beijing 100050 China Department of Neurosurgery Xiamen Fifth Hospital Xiamen University Xiamen Fujian 361101 China

Background：Recent clinical and preclinical studies have suggested that deep brain stimulation （DBS） can be used as a tool to enhance cognitive functions.The aim of the present study was to investigate the impact of DBS at three separate targets in the Papez circuit,including the anterior nucleus of thalamus （ANT）,the Entorhinal Cortex （EC）,and the fornix （FX）,on cognitive behaviors in an Alzheimer＇s disease （AD） rat model.Methods：Forty-eight rats were subjected to an intrahippocampal injection ofamyloid peptides 1-42 to induce an AD model.Rats were divided into six groups：DBS and sham DBS groups of ANT,EC,and FX.Spatial learning and memory were assessed by the Morris water maze （MWM）.Recognition memory was investigated by the novel object recognition memory test （NORM）.Locomotor and anxiety-related behaviors were detected by the open field test （OF）.By using two-way analysis of variance （ANOVA）,behavior differences between the six groups were analyzed.Results：In the MWM,the ANT,EC,and FX DBS groups performed differently in terms of the time spent in the platform zone （F（2.23） =6.04,P ＜ 0.01）,the frequency of platform crossing （F（2,23） =11.53,P ＜ 0.001）,and the percent time spent within the platform quadrant （F（2,23） =6.29,P ＜ 0.01）.In the NORM,the EC and FX DBS groups spent more time with the novel object,although the ANT DBS group did not （F（2,23） =10.03,P ＜ 0.001）.In the OF,all of the groups showed a similar total distance moved （F（1.42） =1.14,P =0.29）and relative time spent in the center （F（2,42） =0.56,P =0.58）.Conclusions：Our results demonstrated that DBS of the EC and FX facilitated hippocampus-dependent spatial memory more prominently thanANT DBS.In addition,hippocampus-independent recognition memory was enhanced by EC and FX DBS.None of the targets showed side-effects of anxiety or locomotor behaviors.

关键词： Anterior Thalamic Nuclei Deep Brain Stimulation Entorhinal Cortex Fornix Memory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A distinct Entorhinal Cortex to hippocampal CA1 direct circuit for olfactory associative learning

A distinct entorhinal cortex to hippocampal CA1 direct circu...

引用

中国神经科学学会第十二届全国学术会议

作者： Yiding Li Jiamin Xu Yafeng Liu Jia Zhu Nan Liu Wenbo Zeng Ning Huang Malte J.Rasch Haifei Jiang Xiang Gu Xiang Li Minhua Luo Chengyu Li Junlin Teng Jianguo Chen Shaoqun Zeng Longnian Lin Xiaohui Zhang Institute of Neuroscience Chinese Academy of Sciences State Key Laboratory of Cognitive Neuroscience & Learning Beijing Normal University Key Laboratory of Brain Functional Genomics-Ministry of Education East China Normal University Wuhan National Laboratory for Optoelectronics–Huazhong University of Science and Technology State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences State Key Laboratory of Membrane Biology College of Life Sciences Peking University China

Multimodal sensory information from many cortices converges into the hippocampus primarily through the Entorhinal Cortex（EC）. Medial and lateral parts of EC（MEC, LEC） transfer spatial and non-spatial, or "context" and "content" related information, respectively, into the hippocampus, mainly via the indirect and direct circuits: EC layer 2→hippocampal dentate gyrus（DG）→CA3→CA1 path and EC layer 3→CA1 path. In this talk, I will present our recent findings on the synaptic organization of the direct circuit and its critical role in associative learning. Using channelrhodopsin-2 assisted circuit mapping（CRACM）, we first elucidated that LEC layer-3 projection neurons selectively form direct synapses onto a distinct subpopulation of morphologically complex, calbindin-expressing pyramidal cells（PCs） in the dorsal CA1（d CA1）, while MEC neurons homogenously innervate all d CA1 PCs. Optogenetically inactivating the distinct lateral EC–d CA1 connections or the postsynaptic d CA1 calbindin-expressing PC activity retarded associative learning in a simple olfaction-based Go/No-go behavioral task. Further in vivo op-tetrode recording in the d CA1 of behaving mice showed that d CA1 calbindin-expressing PCs developed more selective odor representations in their spike responses than neighboring PCs during learning. Thus, these findings reveal a direct lateral EC→d CA1 circuit that is required for olfactory associative learning.

关键词： Entorhinal Cortex hippocampus CA1 calbindin-expressing pyramidal cell direct circuit associative learning optogenetics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Functional dissociation between the medial and the lateral Entorhinal Cortex of the rat in spatial navigation

Functional dissociation between the medial and the lateral e...

引用

中国神经科学学会第十届全国学术会议

作者： Wei-Ning ZHANG Yan XU Hong-Qun DING Chuan-Xiu BIAN Joram Feldon School of Medical Science ＆ Laboratory Medicine Jiangsu University

Objective Lesion studies in rats have indicated that the Entorhinal Cortex（EC） is involved in spatial learning and memory.The Entorhinal Cortex is regarded as an interface between the hippocampus and neocortex and its essential role is to support normal hippocampal-dependent functions,such as memory of spatial information.However,the exact characteristics of deficits following Entorhinal Cortex damage have not been fully described.The present study investigated,in Wistar rats,the effects of temporary inhibition of the medial or lateral Entorhinal Cortex on the retrieval of a well-learned response pattern and the reversal learning（new learning） of the location of an escape platform in the water maze.Methods Rats were bilaterally infused muscimol（1 μg/0.5 μl,per side） into the medial or lateral Entorhinal Cortex and tested the spatial learning and memory in the water maze.Results Muscimol（1 μg/0.5 μl,per side）infusion into the medial,but not lateral,Entorhinal Cortex markedly impaired the retrieval of spatial memory,and failed to affect reversal learning in the water maze.This impairment was temporary,such that the deficits in retrieval of spatial memory were restricted to the day of infusion.Conclusion Our data demonstrates that retrieval of spatial memory requires the involvement of neuronal activity of the medial but not lateral Entorhinal Cortex,suggesting a functional dissociation between the medial and lateral Entorhinal Cortex in spatial navigation.

关键词： Entorhinal Cortex muscimol temporary inhibition spatial learning and memory water maze

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Histamine Influences Spatial Learning by Acting at the Histamine H1 and H3 Receptors in the Rat Medial Entorhinal Cortex

Histamine Influences Spatial Learning by Acting at the Hista...

引用

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：何超胡志安

The Entorhinal Cortex（EC）mediates the majority of the connections between the hippocampus and other cortical areas and plays a crucial role in learning and memory.Whereas the EC receives a dense histaminergic innervation from the tuberomamillary nucleus of the hypothalamus,the functions of histamine in the EC remain elusive.Here,we showed that histamine acted via histamine H1 receptor to directly depolarize the principal neurons in the superficial layers of the medial EC and enhanced their sensitivity to a depolarizing ramp-like current stimulation.Histamine-induced depolarization was mediated via inhibiting Kir channels and required the activation of protein kinase C.In addition,histamine reduced the frequency but not the amplitude of miniature IPSCs by acting at histamine H3 receptor,while it did not affect the frequency and the amplitude of miniature EPSCs,suggesting that histamine specifically decreases spontaneous GABA release on to principal neurons.The Inhibition of spontaneous GABA release by histamine H3 receptor activation was mediated by voltage-gated Ca2+channels and extracellular Ca2+.However,histamine did not influence the excitability of the principal neurons in the deep layers of the medial EC,thus indicating that the histamine-elicited excitation is layer-specific.Furthermore,microinjection of the histamine H1,H3,but not H2,receptor antagonist into the superficial,but not deep,layers of medial EC impaired rat spatial learning as assessed by water maze tasks,while they did not affect the motor function and exploratory activity in an open-field.Together,our study indicates that histamine plays an important role in spatial learning by regulating neuronal excitability and synaptic transmission in the superficial layers of the medial EC.

关键词： histamine Entorhinal Cortex histamine H1 receptor histamine H3 receptor spatial learning

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Low frequency stimulation of Entorhinal Cortex inhibits hippocampal neuronal activities and fast kindling seizures

Low frequency stimulation of entorhinal cortex inhibits hipp...

引用

中国神经科学学会第十届全国学术会议

作者： Zheng-hao XU Yi WANG An-Feng TAO Ceng-Lin XU Miao-Miao JIN Kai ZHONG Shi-Hong ZHANG Zhong CHEN Zhejiang University Department of Pharmacology Key Laboratory of Medical Neurobiology of the Ministry of Health of China and Zhejiang Province Key Laboratory of Neurobiology College of Pharmaceutical Sciences School of Medicine

Objective The Entorhinal Cortex（EC） is a potential target for low frequency stimulation（LFS,1Hz）treatment of temporal lobe epilepsy（TLE）.However,the neuronal basis of LFS at EC is unclear.Basing on the anatomy of entorhinal-hippocampal circuit,the present study was designed to determine whether LFS at EC interfere with hippocampal neural activities and seizures.Method The effects of LFS at EC on the neural activity of dentate gyrus（DG） were examined using single-unit extracellular recordings in anesthetized rats and mice.The effect of LFS at EC on hippocampal fast kindling seizures was tested.Results The spontaneous activity of the majority neurons（104/115 neurons） in DG were repeatedly interrupted as soon as LFS-on and quickly backed to baseline after LFS-off.EC stimulation pulse mainly induced two distinct types of neural response in DG:the commonly observed type（81/115neurons） displayed a 179±12 ms inhibition of neural firing 13±2 ms after stimulation;the second type（23/115 neurons）firstly displayed an excitatory response and then a 130±15 ms inhibition of neural firing 25±5 ms after stimulation.This effect of EC stimulation on DG neural activities were mimicked by stimulation of the main projections from the EC to DG（perforant pathway,PP） or optogenetic selective stimulation of axonal projections in DG.LFS of EC also interrupted neurons in hippocampal CA3 and CA1 but not in substantia nigra.Furthermore,LFS of EC immediately after kindling stimulation but not after the cessation of afterdischarge interfered with kindling and kindled seizures.Conclusion LFS of EC directly interrupted the spontaneous activity hippocampal neural activities,which may through its axonal projections to the hippocampus;and LFS of EC interferes with hippocampal fast kindling seizures in a time-dependent aspect.These results suggest EC is a promising target for closed-loop LFS treatment of TLE.

关键词： epilepsy low-frequency stimulation Entorhinal Cortex kindling

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Exposure to extremely low frequency magnetic field alters the calcium dynamics of rat Entorhinal Cortex neurons via a calcium channel-independent mechanism

Exposure to extremely low frequency magnetic field alters th...

引用

中国神经科学学会第十届全国学术会议

作者： Fen-Lan LUO Nian YANG Zhi-An HU Jun ZHANG Department of Physiology Third Military Medical University

Previous studies have revealed that the extremely low frequency magnetic field(ELF-MF) may affect cognitive functions through influencing several important cognitive-related brain regions,including prefrontal cortex(PFC) and hippocampus.The correct execution of cognitive functions depends on the activity of the whole relevant neural circuitry,in which Entorhinal Cortex(EC) is one of essential structures besides the PFC and the hippocampus and has been recognized as the major relay station between hippocampus and neocortex.Recently,accumulating studies have documented that EC goes beyond being a simple throughput interface between hippocampus and neocortex but plays an active and essential role in a large scale of cognitive processes.And intriguingly,in EC,the excitability of neurons and the neurons’ calcium dynamics plays a critical role in the execution of EC-related cognitive functions.Therefore,in the present study,the effects of 24 hours’ ELF-MF exposure on EC are investigated at first time.Through employing primary culture,whole-cell recording,and calcium imaging,we first show that the ELF-MF exposure does not influence the inward,transient outward,and sustained outward components of the whole-cell currents evoked by voltage steps in EC neurons.The activity and inactivation characteristics of high voltage activated(HVA) and low voltage activated(LVA) calcium channel are also not influenced by the ELF-MF exposure.But importantly,the ELF-MF exposure reduces the maximal amplitude of the high-K evoked calcium dynamics response raised from the unchanged intracellular basal calcium level.Taken together,the ELF-MF does not change the excitability of EC neurons,but alters the intracellular calcium dynamics.Furthermore,after exhausting the intracellular calcium stores with thapsigargin,no difference in the maximal amplitude of the high-K evoked calcium dynamics in EC neurons between the ELF-MF and sham exposure groups is observed.These results suggest that ELF-MF exposure may influence the EC neurons’ functions throu

关键词： extremely low frequency magnetic field Entorhinal Cortex neuronal excitability calcium channel calcium dynamics

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Serotoninergic modulation of picrotoxin-induced epileptiform activity in entorhinal-hippocampal cortex

Serotoninergic modulation of picrotoxin-induced epileptiform...

引用

中国神经科学学会第十届全国学术会议

作者： Hui-Jie SHAO Ming-Po YANG You-Sheng SHU Kai-Yan WANG Department of Neurology Huashan HospitalFudan University Institute of Neuroscience State Key Laboratory of NeuroscienceShanghai Institutes for Biological SciencesChinese Academy of Sciences

Objective We investigated the regulatory effect of 5-HT receptors on spontaneous epileptiform activity in cortical slices.Methods We performed whole-cell recording and extracellular recording from entorhinal-hippocampal slices maintained in vitro.Spontaneous epileptiform activities could be obtained in the presence of picrotoxin,a GABA receptor blocker.We then examined the role of 5-HT and its receptor agonists or antagonists in regulating these epileptiform events.Results Bath application of 5-HT reduced the occurrence frequency of epileptiform activity,and this inhibitory effect could be completely blocked by the pretreatment of 5-HT receptor antagonist WAY-100635.Further experiments in slices obtained from 5-HT knockout mice revealed that 5-HT prolonged the duration of individual epileptiform discharges,and this excitatory effect could be mimicked by 5-HT receptor agonist TCB-2 and blocked by the antagonist ketanserin.Conclusion Our results indicate that activation of 5-HT receptors mediates the inhibitory effect of 5-HT on epileptiform activities,while activation of 5-HT receptors promote the induction of long-lasting epileptic discharges.

关键词： serotonin receptor 5-HT1A 5-HT2A epilepsy Entorhinal Cortex

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Influence of activities in arousal system on the excitability of principal neurons of Entorhinal Cortex and memory

Influence of activities in arousal system on the excitabilit...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yang LI,Jianxia XIA,Xingshu CHEN,Jiali WANG,Jie YAN,Zhongxiang YAO,Zhian HU~* Department of Physiology,Third Military Medical University,Chongqing,400038,China

The Entorhinal Cortex(EC) has long been regarded as a bidirectional gateway between higher cortical areas and the hippocampus.Orexins(hypocretins) are wake-promoting neuropeptides which are mainly produced by the neurons within the lateral hypothalamus.Orexin system includes two separate peptides orexin-A and orexin-B proteolytically derived from the same precursor protein and two specific G-protein-coupled receptors OX1R and OX2R.In the present study,we firstly investigated the electrophysiological effects of orexin-A on stellate neurons and pyramidal neurons in live brain slices of the EC using whole-cell patch-clamp recordings,and then observed signaling cascades triggering the ionic and synaptic mechanisms involved in orexin-A.The results show as follow:Orexin-A depolarizes stellate and pyramidal neurons in the superficial layers of EC throμgh effects on K channels and nonselective cation conductances.The excitatory action of orexin-A involves OX1Rs and PKC mediates both the inhibition of IK and activation of NSCC induced by orexin-A.Orexin-A enhances both excitatory and inhibitory synaptic transmissions in the superficial layers of EC via OX1Rs.In summury,the studies reported here provide evidence that the direct excitatory effects of orexin-A on principal neurons in the superficial layers of rat EC are mediated by the activation of OX1Rs and PKC signaling cascades,which inhibit sustained K current and activate NSCC.In addition, orexin-A enhances both excitatory and inhibitory synaptic transmissions in the EC via OX1Rs.

关键词： orexin Entorhinal Cortex learning and memory

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

“Therapeutic time window” phenomena of low-frequency stimulation at Entorhinal Cortex for amygdaloid-kindling seizures in rats

“Therapeutic time window” phenomena of low-frequency stimu...

引用

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zheng-Hao XU~1 Deng-Chang WU~1 Qi FANG~1 Kai ZHONG~1 Shuang WANG~(1 2) Hong-Liu SUN~1 Shi-Hong ZHANG~1 ZhongCHEN~(1 *) 1 Institute of Neuroscience Department of Pharmacology College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China 2 Department of Neurology 2nd Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

＜正＞Objective Closed-loop stimulation mode for brain stimulation has been considered as a new option for the treatment of intractable epilepsy.However,it is still unclear where and when to deliver low-frequency stimulation（LFS） in closed-loop stimulation mode.The present study was designed to determine whether LFS of the Entorhinal Cortex（EC） have anticonvulsive effect,and whether the onset of LFS given at different time points plays different roles.Methods We evaluated the effect of LFS initiated at different times after kindling stimulation on amygdaloid kindling and kindled seizures in rats.Results Immediate and 4 s-delayed LFS initiated during AD inhibited kindling and kindled seizures,and the 4 s-delayed LFS had a stronger effect than immediate LFS.However,neither 10 s-delayed LFS nor LFS delivered after the cessation of AD had any anticonvulsive effect.On the other hand,the immediate and 4 s-delayed LFS groups showed less decrease in AD threshold,and an increase in the current intensity difference between AD threshold and generalized seizure threshold.Conclusions our results provide direct evidence that the EC is a promising target for LFS to interfere with epilepsy.Furthermore,we first confirms that,even in the duration of afterdischarge,there exists a ’time window’ for LFS treatment,indicating optimization of stimulation timing is crucial for the closed-loop stimulation mode.

关键词： kindling epilepsy brain stimulation low-frequency stimulation closed-loop stimulation Entorhinal Cortex

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Brain imaging of mild cognitive impairment and Alzheimer's disease

引用

Neural Regeneration Research 2013年第5期8卷 435-444页

作者： Changhao Yin Siou Li Weina Zhao Jiachun Feng Department of Neurology the First Hospital of Jilin University Department of Neurology Hongqi Hospital Mudanjiang Medical University

The rapidly increasing prevalence of cognitive impairment and Alzheimer＇s disease has the potential to create a major worldwide healthcare crisis. Structural MRI studies in patients with Alzheimer＇s disease and mild cognitive impairment are currently attracting considerable interest. It is extremely important to study early structural and metabolic changes, such as those in the hippocampus, Entorhinal Cortex, and gray matter structures in the medial temporal lobe, to allow the early detection of mild cognitive impairment and AIzheimer＇s disease. The microstructural integrity of white matter can be studied with diffusion tensor imaging. Increased mean diffusivity and decreased fractional anisotropy are found in subjects with white matter damage. Functional imaging studies with positron emission tomography tracer compounds enable detection of amyloid plaques in the living brain in patients with Alzheimer＇s disease. In this review, we will focus on key findings from brain imaging studies in mild cognitive impairment and Alzheimer＇s disease, including structural brain changes studied with MRI and white matter changes seen with diffusion tensor imaging, and other specific imaging methodologies will also be discussed.

关键词： neural regeneration neuroimaging mild cognitive impairment Alzheimer＇s disease diffusion tensor imaging fractional anisotropy Entorhinal Cortex hippocampus magnetic resonance imaging photographs-containing paper neuorregeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：