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Biological aspects on the cultures of the entomophthoralean fungus Pandora delphacis grown on broomcorn millets

Science Bulletin 2003年第17期48卷 1816-1821页

作者： FENG Mingguang1,2 & LIANG Yong1 1. Institute of Microbiology, College of Life Sciences, Zhejiang Univer-sity, Hangzhou 310029, China 2. Institute of Applied Entomology, College of Agricultural Sciences and Biotechnology, Zhejiang University, Hangzhou 310029, China Institute of Microbiology College of Life Sciences Zhejiang University Hangzhou 310029 China Institute of Applied Entomology College of Agricultural Sciences and Biotechnology Zhejiang University Hangzhou 310029 China

A novel method was developed to use glutinous broomcorn millets (Panicum miliaceum L.) as solid substrate to make cultures of the entomophthoralean fungus Pandora delphacis specifically pathogenic to planthoppers, leafhop-pers and aphids. Steamed millets with water content of 45% were inoculated with a liquid culture of P. delphacis at a ratio of 20% (v/w) and then incubated at 25℃ and L:D 12:12. The millets cultured for 3—17 d exhibited high potential for co-nidial production. The 5-d-old millet culture sporulated most abundantly, discharging up to 17.12 (1.31) 104 conidia/ millet. The cultures incubated for 7—11 d also had a satis-factory sporulation capability, yielding 13.00—13.90 104 conidia/millet. Compared to 2.32 (0.34) 104 conidia dis-charged from each of Myzus persicae adults killed by P. del-phacis and a ≤60-h duration of sporulation, each of the millets cultured for 5—11 d produced 5.6—7.4 times more conidia with an over doubled duration for conidial discharge (144 h). Among 106 M. persicae adults exposed to the shower of conidia discharged from the cultured millets, a total mor-tality of 69.8% caused by P. delphacis infection was observed within 7 d after exposure, but no death was attributed to the fungal infection in the aphids unexposed. The results indi-cate that the millet cultures of P. delphacis are biologically similar to aphid cadavers killed by the same fungus. Due to the superiority of the cultured millets to the cadavers in sporulation potential and duration, the method for making cultures of P. delphacis on the broomcorn millets is highly recommended for use in study of entomophthoralean fungi for microbial control. This is the first report on the success of the solid culture of Pandora species on cereals.

关键词： entomophthorales Pandora delphacis broomcorn millets solid culture sporulation capability aphid infectivity.

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Evidence for transmission of aphid-pathogenic fungi by migratory flight of Myzus persicae alates

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Chinese Science Bulletin 2002年第23期47卷 1987-1989页

作者： Chun Chen Mingguang Feng Zhejiang Univ Inst Microbiol Coll Life Sci Hangzhou 310029 Peoples R China

The alates of the green peach aphid, Myzus persicae, were daily trapped from the air from late October through early January and carried to laboratory for determination of fungal infection by individually rearing them for 7 d on detached cabbage leaves in Petri dishes. Among 760 alates trapped, 266 (35%) were found carrying various fungal pathogens, 87.3% of them died due to mycosis during the first 3-day period of rearing and the rest died in the following two days. Most of the deaths of the alates were attributed to entomophthoralean fungi, taking 94.4%, and the rest were the hyphomycetous fungus Beauveria bassiana. Among the entomophthorales-killed alates, P. neoaphidis took a proportion of 66.1%, Z. anhuiensis 22.6%, E. planchoniana 9.7%, and N. fresenii 1.6%, respectively. Two alates were found suffering from cross infection of two fungal species, i.e. P. neoaphidis with Z. anhuiensis and N. fresenii, respectively. The results represent the first report on transmission of aphid-pathogenic fungi

关键词： Myzus persicae alates migratory flight entomophthorales Beauveria bassiana aphid mycosis transmission initial inoculum.

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Isolation and Identification of Pandora neoaphidis and Its Pathogenicity against Turnip Aphid(Lipaphis erysimi)

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Plant Diseases and Pests 2010年第6期1卷 43-45页

作者：贾春生韶关学院英东生物工程学院广东韶关512005

[Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid （Lipaphis erysimi）.[Method] Based on the morphological characteristics,the species of pathogenic fungus was *** shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,（24.7±1.4）μm×（10.7±0.9）μm,L/D=2.3±*** conidia had the similar shape with the primary ones,（18.6±2.1）μm×（13.3±1.3）μm,L/D=1.4±*** body was like mycelium with the diameter of （10.6±0.8）μ*** had palmate branch with the diameter of （10.0±0.9）μ*** was not branched,which had rough base with the diameter of （19.2±1.7）μm,and gradually became more angular towards the apex with the diameter of （8.0±0.9）μm at *** was like monohyphal shape with the diameter of （21.0±3.0）μm at base,the terminal apex had regular discoid *** resting spores were *** lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid.

关键词： Pandora neoaphidis Pathogenicity Turnip aphid Entomopathogenic fungus entomophthorales

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