检索结果-南通市图书馆

Purification and characterization of iron-cofactored superoxide dismutase from enteromorpha linza

Chinese Journal of Oceanology and Limnology 2013年第6期31卷 1190-1195页

作者：吕明生蔡阮鸿王淑军刘兆普焦豫良房耀维张小昕 Marine School Huaihai Institute of Technology College of Resources and Environmental Science Nanjing Agricultural University

A superoxide dismutase was purified from enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose column, and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase （E/SOD） was purified 103.6-fold, and a yield of 19.1% and a specific activity of 1 750 U/rag protein were obtained. The SDS-PAGE exhibited E/SOD a single band near 23 kDa and the gel filtration study showed E/SOD＇s molecular weight is near 46 kDa in nondenatured condition, indicating it＇s a homodimeric protein. E/SOD is an iron-cofactored superoxide dismutase （Fe-SOD） because it was inhibited by hydrogen peroxide, insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35℃, and it still had 29.8% relative activity at 0℃, then E/SOD can be classified as a cold-adapted enzyme. E/SOD was stable when temperature was below 40℃ or the pH was within the range of 5 10. The first 11 N-terminal amino acids orE/SOD were ALELKAPPYEL, comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.

关键词： iron-cothctored superoxide dismutase enteromorpha linza purification enzymatic characterization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chemical Characteristics and Anticoagulant Activities of Two Sulfated Polysaccharides from enteromorpha linza (Chlorophyta)

引用

Journal of Ocean University of China 2013年第1期12卷 175-182页

作者： QI Xiaohui MAO Wenjun CHEN Yin CHEN Yanli ZHAO Chunqi LI Na WANG Chunyan Key Laboratory of Marine Drugs Ministry of Education Institute of Marine Drug and Food Ocean University of China

Two sulfated polysaccharides, designated MP and SP, were extracted from the marine green alga enteromorpha linza using hot water and then purified using ion-exchange and size-exclusion chromatography. The anticoagulant activities of MP and SP were examined by determination of their activated partial thromboplastin time （APTT）, thrombin time （TT） and prothrombin time （PT） using human plasma. Results showed that MP and SP were composed of abundant rhamnose with small amounts of xylose and glucuronic acid, whereas SP also contained a small amount of galactose. Approximate molecular weights of MP and SP were 535 and 502 kDa, respectively. As compared with SP, MP had higher contents of sulfate ester （19.0%） and uronic acid （14.9%）. The MP mainly consisted of （1→4）-linked rhamnose residues with partially sulfated groups at the C-3 position, and small amounts of （1→3, 4）-linked rhamnose, （1→2,4）-linked rhamnose, （1→4）-linked glucuronic acid and （1→4）-linked xylose residues. The SP contained abundant （1→4）-linked rhamnose with minor amounts of （1→3）-linked rhanmose, （1→3, 4）-linked rhamnose, （1→2, 4）-linked rhanmose, （1→4）4inked glucuronic acid, （1→4）-linked xylose, and （1→3）-linked galactose residues. The sulfate groups were mainly located at C-3 of （1→4）-linked rhamnose residues. Both MP and SP, in particular the former, effectively prolonged APTT and TT. This work demonstrates that MP and SP have unique structural characteristics distinct from those of other sulfated polysaccharides from enteromorpha. The MP is a potential source of anticoagulant, and the difference in anticoagulant activities of the two sulfated polysaccharides is directly linked to the discrepancy of their chemical features.

关键词： Marine green alga enteromorpha linza sulfated polysaccharide chemical characteristic anticoagulant activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：