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A Study on Detecting and Identifying enteric pathogens With PCR

Biomedical and Environmental Sciences 2004年第1期17卷 109-120页

作者： JUN-WENLI XIU-QUANSHI FU-HUANCHAO XIN-WEIWANG JIN-LAIZHENG NONGSONG InstituteofHealthandEnvironmentalMedicine 1DaLiRoadTianjin300050China

Objective To develop a rapid and definite diagnostic test of bacterial enteritis caused by pathogenic enterobacteria, the most frequent etiologic agent of infectious enteritis in the world. Methods A set of conventional PCR assays were applied to detect and identify salmonella, shigella, and E. coli O157:H7 directly from pure culture and fecal samples. The general primers of pathogenic enterobacteria were located on the uidA gene, which were found not only in E. coli nuclear acid, but also in Shigella and salmonella genes. Shigella primer was from ipaH gene whose coded invasive plasmid relative antigen existed both in plasmid and in genome. The primers of salmonella were designed from the 16SrRNA sequence. The primer of *** O157:H7 was taken from eaeA gene. Five random primers were selected for RAPD. The detection system included common PCR, semi-nested PCR and RAPD. Results This method was more sensitive, specific and efficient and its processing was rapid and simple. For example, the method could be used to specifically detect and identify salmonella, shigella, and E. coli O157:H7, and its sensitivity ranged from 3 to 50 CFU, and its detection time was 4 hours. Conclusion This PCR method, therefore, can serve as a routine and practical protocol for detecting and identifying pathogenic microorganisms from clinical samples.

关键词： enteric pathogen Detection Identification PCR

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Samonella typhi infection-related appendicitis:A case report

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World Journal of Clinical Cases 2021年第29期9卷 8782-8788页

作者： Bo-Hao Zheng Wei-Ming Hao Hung-Chen Lin Guo-Guo Shang Han Liu Xiao-Jian Ni Department of General Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Shanghai Medical College Fudan UniversityShanghai 200032China Department of Pathology Zhongshan HospitalShanghai 200032China

BACKGROUND Acute appendicitis is one of the most common acute abdominal emergencies around the world,which is always associated with *** with Salmonella typhi,an enteric pathogen,is a rare cause of acute *** here report a patient with acute appendicitis associated with Samonella typhi infection,accompanied with spleen and kidney infarction,providing a rare example for a common surgical *** SUMMARY A 25-year-old Pakistani man presented to the hospital with a 3-d history of fevers,vomiting,and abdominal *** tomography(CT)revealed a thickened intestinal wall of the ileocecal junction with multiple enlarged lymph nodes *** was diagnosed with acute appendicitis and received laparoscopic appendectomy,which showed mild inflammation of the *** the surgery,the patient presented again with a high fever(>39℃)and diarrhea.A CT angiography scan indicated spleen and kidney *** to the blood culture,the diagnosis was finally clear to be Samonella typhi *** pyrexia and enteric symptoms were relieved after the application of intravenous *** This case,characterized by the combination of Salmonella typhi infection,acute appendicitis,and renal and splenic infraction,serves as a rare example for a common surgical emergency.

关键词： Acute appendicitis Samonella typhi infection enteric pathogen Laparoscopic appendectomy Case report

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Post-infectious irritable bowel syndrome:Mechanistic insights into chronic disturbances following enteric infection

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World Journal of Gastroenterology 2014年第14期20卷 3976-3985页

作者： Jennifer K Beatty Amol Bhargava Andre G Buret Department of Biological Sciences University of Calgary

Irritable bowel syndrome(IBS)is a commonly encountered chronic functional gastrointestinal(GI)*** 10%of IBS patients can trace the onset of their symptoms to a previous a bout of infectious *** appearance of new IBS symptoms following an infectious event is defined as ***,with the World Health Organization estimating between 2 and 4 billion cases annually,infectious diarrheal disease represents an incredible international healthcare ***,compounding evidence suggests many commonly encountered enteropathogens as unique triggers behind IBS symptom generation and underlying pathophysiological features.A growing body of work provides evidence supporting a role for pathogen-mediated modifications in the resident intestinal microbiota,epithelial barrier integrity,effector cell functions,and innate and adaptive immune features,all proposed physiological manifestations that can underlie GI abnormalities in *** pathogens must employ a vast array of machinery to evade host protective immune mechanisms,and illicit successful ***,the impact of infectious events on host physiology can be multidimensional in terms of anatomical location,functional scope,and *** review offers a unique discussion of the mechanisms employed by many commonly encountered enteric pathogens that cause acute disease,but may also lead to the establishment of chronic GI dysfunction compatible with IBS.

关键词： Post-infectious irritable bowel syndrome Infectious diarrhea enteric pathogen Inflammatory disorders Immune alterations

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