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Oxidative Damage to Lung Tissue and Peripheral Blood in endotracheal PM_(2.5)-treated Rats

Biomedical and Environmental Sciences 2009年第3期22卷 223-228页

作者： ZHI-QING LIN ZHU-GE XI DAN-FENG YANG Fu-HUAN CHAO HUA-SHAN ZHANG WEI ZHANG HUANG-LIANG LIU ZAI-MING YANG RU-BAO SUN Institute of Health and Environmental Medicine Tianjin 300050 China State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control Tianjin 300071 China

Objective To investigate the oxidative damage to lung tissue and peripherial blood in PM2.5-treated rats. Methods PM2.5 samples were collected using an auto-sampling instrument in summer and winter. Treated samples were endotracheally instilled into rats. Activity of reduced glutathione peroxidase （GSH-Px） and concentration of malondialdehyde （MDA） were used as oxidative damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. DNA migration length （μm） and rate of tail were used as DNA damage biomarkers of lung tissue and peripheral blood detected with the biochemical method. Results The activity of GSH-Px and the concentration of MDA in lung tissue significantly decreased after exposure to PM2.5 for 7-14 days. In peripheral blood, the concentration of MDA decreased, but the activity of GSH-Px increased 7 and 14 days after experiments. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. The DNA migration length （μm） and rate of tail in lung tissue and peripheral blood significantly increased 7 and 14 days after exposure to PM2.5. The two indicators had a dose-effect relation and similar changing tendency in lung tissue and peripheral blood. Conclusion PM2.5 has a definite oxidative effect on lung tissue and peripheral blood. The activity of GSH-Px and the concentration of MDA are valuable biomarkers of oxidative lung tissue damage induced by PM2.5. The DNA migration length （μm） and rate of tail are simple and valuable biomarkers of PM2 5-induced DNA damage in lung tissues and peripheral blood. The degree of DNA damage in peripheral blood can predict the degree of DNA damage in lung tissue.

关键词： PM2.5 endotracheal instillation Oxidative damage Oxidative DNA damage Biomarker DNA repair

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Oxidative Damage on Lung Tissue and Peripheral Blood in endotracheally Treated Rat by PM2.5

Oxidative Damage on Lung Tissue and Peripheral Blood in Endo...

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2007中国科协年会

作者： Lin Zhiqing~1 Xi Zhuge~(*1,2) Yang Danfeng~1 Chao Fuhuang~1 Zhang Huashan~1 Zhang Wei~1 Liu Huangliang~1 Yang Zaiming~1 Sun Rubao~1 1.Institute of Health and Environmental Medicine,Tiqnjin 300050,China 2.State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control,P.R.China

Objective Since epidemiological studies have firmly implied the environmental atmospheric fine particle matters(PM)as potential etiological factor involved in the excess of mortality by lung end cardiovascular diseases, experimental studies have been performed to investigate the oxidative damage of lung tissue and peripheral blood. Methods PM samples were collected by auto-sampling instrument in summer and *** treated samples were endotracheally instillatilled on *** activity of reduced glutathione peroxidase(GSH-Px)and the concentration of malondialdehyde(MDA)were used as the oxidative damage biomarkers of lung tissue and peripheral blood detected by the biochemistry *** DNA migeration length(μm)and rate of tail were used as the DNA damage biomarkers of lung tissue and peripheral blood detected by the biochemistry *** The activity of GSH-Px and the concentration of MDA in the lung tissue had the significant decrease after 7 and 14 days exposure of *** peripheral blood,the concentration of MDA decreased;but the activity of GSH-Px increased in 7 days and increased in 14 days exposure *** two indicators had the dose-effect relationships,and the similar changeful tendency in the lung tissue and peripheral *** DNA migeration length(μm)and rate of tail in the lung tissue and peripheral blood had the significant increase after 7 and 14 days exposure of *** two indicators had the dose-effect relationships,and the similar changeful tendency in the lung tissue and peripheral *** PM has definite oxidative effect on lung tissue and peripheral *** activity of GSH-Px and the concentration of MDA are the valuable biomarkers of the oxidative damage of lung tissues induced by *** DNA migeration length(μm)and rate of tail are the simple and valuable biomarkers of the DNA damage of lung tissues and peripheral blood induced by PM,we can detect the degree of DNA damage in peripheral blood to predict the degree o

关键词： PM2.5 endotracheal instillation Oxidative damage Oxidative DNA damage Biomarker DNA repair

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