检索结果-南通市图书馆

Dicycloplatin differentially inhibits proliferation of human aortic smooth muscle and Endothelial cells： potential for use in drug-eluting stents

引用

Chinese Medical Journal 2012年第24期125卷 4386-4392页

作者： XU Lian-jun GAO Run-lin WU Chao YE Jue SONG Li QIAN Xin State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China Center for Coronary Heart Disease Department of CardiologyFuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China Center for Endocrine and Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China

Background Dicycloplatin is a relatively safe third generation focused on the effects of dicycloplatin on in vitro proliferation （HASMC） and human aortic Endothelial cells （HAEC）. platinum-complex anti-cancer drug. The present study and apoptosis of human aortic smooth muscle cells Methods Proliferation of HASMC and HAEC, DNA content, and cellular levels of proliferation- and apoptosis-related proteins were assessed using the （3-（4,5-dimethylthiazol-2-yl）-5-（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）- 2H-tetrazolium）（MTS） assay, flow cytometry and Western blotting assays, respectively. Results Dicycloplatin at 10 ng/ml significantly inhibited HASMC proliferation, however, 10 μg/ml were required to significantly inhibit HAEC proliferation. Cell cycle analysis showed that dicycloplatin was a non-specific inhibitor of the cell cycle. Although dicycloplatin significantly decreased proliferating cell nuclear antigen （PCNA） expression in HASMC at all concentrations tested, it did not significantly affect PCNA expression in HAEC; Bax and p53 protein expression was upregulated in dicycloplatin groups. Conclusions Dicycloplatin at nanogram concentrations significantly inhibits HASMC proliferation, although the effect is relatively weaker than that of sirolimus. In contrast, the effect of dicycloplatin on inhibition of HAEC proliferation is much less pronounced than that on HASMC. The latter characteristics point to the potential for use of dicycloplatin in drug-eluting stents.

关键词： smooth muscle Endothelial cells cell proliferation apoptosis dicycloplatin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Endoplasmic Reticulum Stress-mediated Aldosterone-induced Apoptosis in Vascular Endothelial cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2014年第6期34卷 821-824页

作者：卢金萍李夏金雅磊陈梅香 Department of Internal Medicine & Geriatrics Zhongnan Hospital of Wuhan University

The aim of this study was to examine the effects of endoplasmic reticulum （ER） stress on aldosterone （Aldo）-induced apoptosis of Endothelial cells. Glucose-regulated protein 78 （GRP78） and C/EBP homologous protein （CHOP, a hallmark of ER-associated apoptosis） were used to evaluate ER stress. Western blotting and real-time PCR were used to analyze indicators of ER molecule. Apoptosis was detected by annexin V/propidium iodide staining and flow cytometry. Human umbilical vein endo- thelial cells （HUVECs） were stimulated with different concentrations of Aldo for different durations. Aldo promoted apoptosis of HUVECs and induced ER stress, as evidenced by increased expression of GRP78 and CHOP. siRNA knockdown of CHOP attenuated Aldo-mediated apoptosis. These results in- dicate that ER stress may be involved in Aldo-induced apoptosis of HUVECs.

关键词： endoplasmic reticulum stress apoptosis aldosterone Endothelial cells

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Functional Endothelial cells derived from embryonic stem cells labeled with HIV transactivator peptide-conjugated superparamagnetic nanoparticles

引用

Chinese Medical Journal 2011年第2期124卷 298-303页

作者： GAO Bin FU Wei-guo DONG Zhi-hui FANG Zheng-dong LIU Zhen-jie SI Yi ZHANG Xiang-man WANG Yu-qi Department of Vascular Surgery Zhongshan Hospital Fudan University Shanghai 200032 China Department of General Surgery Sir Run Run Shaw Hospital Zhejiang University Hangzhou Zhejiang 310016 China

Background The development of regenerative therapies using derivatives of embryonic stem （ES） cells would be facilitated by a non-invasive method to monitor transplanted cells in vivo, for example, magnetic resonance imaging of cells labeled with superparamagnetic iron oxide （SPIO） nanoparticles. Although ES cells have been labeled with SPIO particles, the potential adverse effects of the label have not been fully examined. The objective of this study was to determine whether SPIO labeling affects routine ES cell viability, proliferation, or ability to differentiate into functional Endothelial cells （ECs）. Methods Cross-linked iron oxide （CLIO, an SPIO） was conjugated with human immunodeficiency virus transactivator of transcription （HIV-Tat） peptides, and murine ES cells were labeled with either CLIO-Tat, CLIO, or HIV-Tat. After labeling ES cells were cultured for 4 days and FIk-1^＋ ES cells identified and sorted by immunocytochemistry and fluorescence activated cell sorting （FACS）. FIk-1^＋ cells were replated on fibronectin-coated dishes, and ECs were obtained by culturing these for 4 weeks in endothelial cell growth medium supplemented with vascular endothelial growth factor （VEGF）. ES cell viability was determined using trypan blue exclusion, and the proportion of SPIO^＋ cells was evaluated using Prussian blue staining and transmission electron microscopy. After differentiation, the behavior and phenotype of ECs were analyzed by reverse transcription-polymerase chain reaction, flow cytometry, immunocytochemistry, Dil-labeled acetylated low-density lipoprotein （AcLDL） uptake, and Matrigel tube formation assay. Results CLIO-Tat was a highly effective label for ES cells, with 〉96% of cells incorporating the particles, and it did not alter the viability of the labeled cells. ECs derived from CLIO-Tat^＋ ES cells were very similar to murine aortic ECs in their morphology, expression of endothelial cell markers, ability to form vascular-like channels, and scavenging of AcLDL from the culture medium. Conclusions CLIO

关键词： embryonic stem cells Endothelial cells cell differentiation ferumoxides

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibitory Effects of Rap1GAP Overexpression on Proliferation and Migration of Endothelial cells via ERK and Akt Pathways

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2011年第6期31卷 721-727页

作者：李文毅金毕 Lynn A.Cornelius 周斌符晓阳尚丹郑鸿 Department of Vascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Division of Dermatology Department of Internal MedicineWashington University School of MedicineSt. Louis MO 63110 USA

Rapl is expressed in human umbilical vein Endothelial cells （HUVECs）. Rapl-GTPase activating protein （RaplGAP）, with its specific target, Rapl, has been shown to be important in the regulation of many physiological and certain pathological processes. In this study, we investigated the effect of RaplGAP expression on endothelial cell function, or, more specifically, proliferation and migration of Endothelial cells. HUVECs were transfected with pcDNA3.1 （empty vector）, pcDNA3.1 containing Flag-tagged-RaplGAP or Myc-tagged-RaplN17. The proliferation, migration and tube formation were examined and compared among the 3 groups. Expression of Rapl, RaplGAP, extracellular signal-regulated kinase （ERK）, phospho-ERK, Akt, phosphor-Akt was detected by Western blotting. The results showed that the proliferation, migration and tube formation were significantly reduced in RaplGAP- and RaplN17-transfected HUVECs as compared with empty vector-transfected control. These changes were coincident with increased expression of Rap 1GAP and decreased expression of activated Rap l, phospho-ERK and -Akt. After treatment of Rap l GAP-transfected HUVECs with a stimulator of Rapl guanine-nucleotide-exchange factor （RaplGEF） 8CPT-2＇OMe-cAMP, it was found that Rapl activity was decreased as compared with empty vector-transfected control. Pretreatment of HU- VECs with an ERK inhibitor PD98059 or a PI3K inhibitor LY294002 prior to stimulation not only blocked 8CPT-2＇OMe-cAMP-induced phosphorylation of ERK and Akt, but also significantly reduced cell proliferation and migration. Finally, we examined the effect of vascular endothelial growth factor （VEGF） on HUVECs overexpressing RaplGAP. VEGF-stimulated Rapl activity, phosphorylation of ERK and Akt, cyclin D1 expression and cell proliferation were repressed in HUVECs overexpressing RaplGAP as compared to empty vector-transfected Control. Taken together, our findings demonstrate that RaplGAP/Rapl and their downstream effectors regulate proliferation and migration of HUVECs via ERK and Akt pathway

关键词： Rapl-GAP protein Rap 1 Endothelial cells proliferation migration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of rotational culture on morphology,nitric oxide production and cell cycle of Endothelial cells

引用

BIOCELL 2012年第3期36卷 97-103页

作者： CHAOJUN TANG XUE WU LINQI YE XIANG XIE GUIXUE WANG Key Laboratory of Biorheological Science and Technology(Chongqing University) Ministry of EducationChongqing Engineering Laboratory in Vascular ImplantsBioengineering College of Chongqing UniversityChongqing400044China Analytical Center of Yangtze Normal University Chongqing408100China

Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering.However,there are few reports exploring the effects of rotational culture on cell morphology,nitric oxide(NO)production,and cell cycle of the Endothelial cells from human umbili-cal vein on the stent surface.This study focuses on these parameters after the cells are seeded on the stents.Results showed that covering of stents by Endothelial cells was improved by rotational culture.NO produc-tion decreased within 24 h in both rotational and static culture groups.In addition,rotational culture signifi-cantly increased NO production by 37.9%at 36 h and 28.9%at 48 h compared with static culture.Flow cytometry showed that the cell cycle was not obviously influenced by rotational culture.Results indicate that rotational culture may be helpful for preparation of cell-seeded vascular grafts and intravascular stents,which are expected to be the most frequently implanted materials in the future.

关键词： Endothelial cells rotational culture stents nitric oxide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Interactions of primary neuroepithelial progenitor and brain Endothelial cells: distinct effect on neural progenitor maintenance and differentiation by soluble factors and direct contact

引用

Cell Research 2007年第7期17卷 619-626页

作者： Miguel A Gama Sosa Rita De Gasperi Anne B Rocher Gissel M Perez Keila Simons Daniel E Cruz Patrick R Hof Gregory A Elder Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA Departments of Psychiatry Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA Neuroscience Mount Sinai School of Medicine of New York University New York NY Department of Research and Development Service James J. Peters Department of Veterans Affairs Medical Center 130 West Kingsbridge Road Bronx New York 10468 USA

Neurovascular interactions are crucial for the normal development of the central nervous system. To study suchinteractions in primary cultures, we developed a procedure to simultaneously isolate neural progenitor and endothelialcell fractions from embryonic mouse brains. Depending on the culture conditions Endothelial cells were found to favormaintenance of the neuroprogenitor phenotype through the production of soluble factors, or to promote neuronal differ-entiation of neural progenitors through direct contact. These apparently opposing effects could reflect differential cellularinteractions needed for the proper development of the brain.

关键词： differentiation culture Endothelial cells neuroepithelial cells

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biphasic effect of aspirin on apoptosis of bovine vascular Endothelial cells and its molecular mechanism

引用

Acta Pharmacologica Sinica 2007年第3期28卷 353-358页

作者： Qing-quan CHEN Wen-lan LIU Xun GUO Yuan-jian LI Zhao-gui GUO~1 Department of Pharmacology,School of Pharmaceutical Sciences,Central South University,Changsha 410078,China DepartmentofPharmacology SchoolofPharmaceuticalSciencesCentralSouthUniversityChangsha410078China

Aim:To investigate the effect of aspirin on the apoptosis of cultured bovineaortic Endothelial cells(BAEC)and the signal pathways involved in this process.Methods:BAEC were cultured and passaged in Dulbecco’s modified Eagle’smedium culture medium.Morphologic changes and quantification of apoptoticcells were determined using fluorescence microscope after staining the cells withHoechst 33258.Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT)method.DNA fragmentation was visualizedby agarose gel electrophoresis.Phospho-p38 mitogen-activated protein kinase(MAPK)expression was detected by Western blotting.Results:Aspirin at lowconcentrations from 1×10-10mol/L to 1×10-8mol/L decreased the apoptosis andp38 MAPK pbosphorylation induced by H2O2in BAEC,while high doses of aspi-rin(1×10-7-1×10-4mol/L)induced typical apoptotic changes in BAEC and stimu-lated the expression of phospho-p38 MAPK in a concentration-dependent manner.SB203580,a specific p38 MAPK inhibitor,blocked such effects.Conclusion:Aspirin exhibits a biphasic effect on the apoptosis in BAEC,reducing apoptosisat low concentration and inducing apoptosis at high concentration,p38 MAPKmay be an important signal molecule mediating the effects of aspirin.

关键词： aspirin hydroperoxide apoptosis mitogen activated protein kinase Endothelial cells

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Expression of Interleukin-15 and Its Receptor on the Surface of Stimulated Human Umbilical Vein Endothelial cells

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2009年第5期29卷 527-534页

作者：刘秀平左玉梅张维娜杨德光熊长云张小舟 College of Life Science and Technology Huazhong University of Science and Technology Hubei Vocational and Technical College Department of Cardiology Southwest Hospital Third Military Medical University Institute of Organ Transplantation Key Laboratory of Organ TransplantationMinistry of Education/Ministry of HealthTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology College of Agriculture Northeast Agricultural University Molecular Targets Development Program National Cancer Institute-Frederick National Institutes of Health Frederick Xianggan Medical College Hospital Huazhong University of Science and Technology

Summary： Human interleukin-15 （hIL-15） is an important cytokine to activate Endothelial cells and can be regulated by many other cytokines. The aim of this study is to examine the ability of interferon-γ （IFN-γ）, and tumor necrosis factor-or （TNF-α to induce the production of human interleukin-15 （hIL-15） and IL-15 receptor （IL-15Rα by human umbilical vein Endothelial cells （HUVECs）. The data are summarized as follows： 1. Northern blot revealed that IL-15 mRNA was up-regulated by IFN-γ and TNF-α 2. Intracellular IL-15 protein was visualized by fluorescence microscopy, whereas the expression of IL-15 on the surface of HUVECs was detected by fluorescence activated cell sorting （FACS）, and no detectable IL-15 in the medium was verified by ELISA. 3. IL-15Rα was detected on the surface of HUVECs by FACS after IFN-α and TNF-α stimulation, whereas Western blotting revealed that the elevated expression on surface IL-15Rα was not due to the increased protein expression. The conclusion demonstrated from our results is that IFN-α and TNF-α play an important role in regulating the expression of IL-15 and IL-15Rα on the surface of HUVECs.

关键词： Endothelial cells interleukin- 15 interleukin- 15 receptor cytokines

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of shear stress on differentiation of stem cells into Endothelial cells

引用

World Journal of Stem cells 2021年第7期13卷 894-913页

作者： Yan Huang Jia-Yi Qian Hong Cheng Xiao-Ming Li Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing 100083China

Stem cell transplantation is an appealing potential therapy for vascular diseases and an indispensable key step in vascular tissue engineering.Substantial effort has been made to differentiate stem cells toward vascular cell phenotypes,including Endothelial cells(ECs)and smooth muscle cells.The microenvironment of vascular cells not only contains biochemical factors that influence differentiation but also exerts hemodynamic forces,such as shear stress and cyclic strain.More recently,studies have shown that shear stress can influence the differentiation of stem cells toward ECs.A deep understanding of the responses and underlying mechanisms involved in this process is essential for clinical translation.This review highlights current data supporting the role of shear stress in stem cell differentiation into ECs.Potential mechanisms and signaling cascades for transducing shear stress into a biological signal are proposed.Further study of stem cell responses to shear stress will be necessary to apply stem cells for pharmacological applications and cardiovascular implants in the realm of regenerative medicine.

关键词： Shear stress Stem cells Cell differentiation Endothelial cells Mechanotransduction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Human vascular smooth muscle cells and Endothelial cells cocultured on polyglycolic acid (70/30) scaffold in tissue engineered vascular graft

引用

Chinese Medical Journal 2007年第15期120卷 1331-1335页

作者： WEN Shao-jun ZHAO Li-min WANG Shen-guo LI Jing-xing CHEN Hua-ying LIU Jie-lin LIU Ya LUO Yi Roo Changizi Central Experiment Unit Beijing Anzhen Hospital Capital Medical University and Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing 100029 China State Key Laboratory of Polymer Physics and Chemistry Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Shunyi Clinic Beijing United Family Hospital Beijing 101300

Background Current prosthetic, small diameter vascular grafts showing poor long term patency rates have led to the pursuit of other biological materials. Biomaterials that successfully integrate into surrounding tissue should match not only the mechanical properties of tissues, but also topography. Polyglycolic acid （70130） has been used as synthetic grafts to determine whether human vascular smooth muscle cells and Endothelial cells attach, survive and secrete endothelin and 6-keto-prostaglandin F1α （6-keto-PGF1α）. Methods Endothelial cells and smooth muscle cells were isolated from adult human great saphenous vein. They were seeded on polyglycolic acid scaffold in vitro separately to grew vascular patch （Groups A and B respectively） and cocultured in vitro to grow into vascular patch （Group C）. Smooth muscle cells and Endothelial cells were identified by immunohistochemical analysis and growth of cells on polyglycolic acid was investigated using scanning electron microscopy. The levels of endothelin and 6-keto-PGF1α in the culturing solutions were examined by radioimmunology to measure endothelial function. Results Seed smooth muscle cells adhered to polyglycolic acid scaffold and over 28 days grew in the interstices to form a uniform cell distribution throughout the scaffold. Then seed Endothelial cells formed a complete endothelial layer on the smooth muscle cells. The levels of endothelin and 6-keto-prostaglandin F1 alpha in the culturing solution were （234±29） pg/ml and （428±98） pg/ml respectively in Group C and （196±30） pg/ml and （346±120） pg/ml in Group B; both significantly higher than in Groups A and D （blank control group, all P〈0.05 ）. Conclusions cells could be grown successfully on polyglycolic acid and retain functions of secretion. Our next step is to use human saphenous vein smooth muscle cells and Endothelial cells to grow tubular vascular grafts in vitro.

关键词： tissue engineering blood vessels Endothelial cells smooth muscle cells saphenous vein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：