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Effect of Taoren Quyu Decoction on human endometrial cells and its anti-endometriosis activity in rats

Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 758-762页

作者： Hai-Zhi Liu Xiao-Xue Han Jia Liu Feng-Cheng Zhu Rui-Man Li Department of Gynaecology and Obstetrics the First Affiliated Hospital of Jinan UniversityGuangzhou 510630Guangdong ProvinceChina

Objective: To study the effect of Taoren Quyu Decoction(TQD) on endometrial cells in patients with endometriosis(EMs) and EMs in rats. Methods: A total of 60 female Wistar rats were randomly divided into 4 groups, namely, normal group, model group, positive group and TQD group, each group having 15 rats. Except the normal group, EMs model was established in the other three groups by transplanting the rat autologous endometrium. After 4 weeks of intragastric administration, blood, eutopic and ectopic endometrial tissues of rats in each group were collected to detect the serum levels of estrogen(E2), cancer antigen 125(CA125), endometrial antibody(EMAb), and expressions of microvessel density(MVD), vascular endothelial growth factor(VEGF) and angiopoietin(Ang-2). The volume of endometriosis cyst was determined simultaneously. For the in vitro culture of human endometrial cells, 4 groups, namely, normal group, model group, positive group and TQD group were used. The positive group and TQD group were treated with danazol and TQD respectively. Then 24 h after the treatment, the expressions of survivin and tumor suppressor gene(p53) of each group were detected. Results: The volumes of the endometriosis cysts in the positive group and the TQD group were significantly reduced compared with the model group(P0.05). Con

关键词： Taoren Quyu Decoction Endometriosis endometrial cell Rats Angiogenesis Apoptosis

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Effects of Yimu Shenghuasan Preparation on the Cytochrome P450 in endometrial cells and Immune Function of Dairy Cows

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Agricultural Sciences in China 2010年第10期9卷 1497-1503页

作者： DU Jin-liang QIN Jian-hua CHU Jing-sheng XU Li-na MA Yu-zhong College of Animal Science and Technology Agricultural University of Hebei Baoding 071001 IER.China

In order to investigate the mode of action ofYimu Shenghuasan preparation in endometrial cells of dairy cows, the primary cultured endometrial cells in cows were isolated and the inflammatory models were made by lipopolysaccharide （LPS） induction. The inflammatory cells were treated with gradient concentration of herbal medicine preparation, Yimu Shenghuasan for 48 and 72 h. The expression of cytochrome P450 （CYP450） was detected by Western blot. The amounts of IgG and lgA in sera were also detected in the endometritis of dairy cows. The expression level of CYP450 in the endometrial cells of dairy cow was increased gradually, and the amounts of IgG, IgA were increased significantly as compared with those in the control group. The expression level of CYP450 in the inflammatory cells was increased significantly in the treatment of 2 000 μg mL^-1 of Yimu Shenghuasan after 48 h of treatment.

关键词： dairy cow endometrial cell CYP450 Yimu Shenghuasan preparation immune function

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Differentially Gene Expression Profile Related to Inflammation in endometrial cells Induce by Lipopolysaccharide

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Journal of Reproduction and Contraception 2009年第1期20卷 27-34页

作者： Li-hua QIN Ruo-guang WANG Sheng LI Chun-mei LI Guangzhou University of TCM College of the Second Clinical Medicine Guangzhou 510405 China Institute of Combined Traditional Chinese and Western Medicine Hunan University of TCM Changsha 410007.China

Objective To investigate differentially expressed genes related to inflammation in endometrial cells induced by Lipopolysaccharide（LPS）. Methods Normal endometrium in the proliferative phase of specimen from 3 cases for the experiment was collected. The LPS group were treated with 50 μg/ml LPS. Total RNA was extracted using Trizol reagent from cells. RNA quality was assessed by determining the OD260/280 ratio by agarose gel electrophoresis, the chip was scanned by laser scanner. The acquired was analyzed. Results A total of differentially expressed genes were found, these genes were relative to many aspects. Among them, the expression of genes involved in inflammation were up-regulated by LPS, such as overexpression of IL-1β, 8, etc. Conclusion The results indicates that inflammation-related genes may be one of the mechanisms of abnormal uterine bleeding by LPS-induced.

关键词： gene expression profile lipopolysaccharide（LPS） endometrial cell inflammation

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