检索结果-南通市图书馆

维普期刊数据库

Roles of the Nanog protein in murine F9 embryonal carcinoma cells and their endoderm-differentiated counterparts

在线全文

学校读者我要写书评

暂无评论

Cell Research 2006年第7期16卷 641-650页

作者： Yanmei Chen~1 Zhongwei Du~2 Zhen Yao~1 ~1Laboratory of Molecular and Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Graduate School of the Chinese Academy of Sciences,320 Yueyang Road,Shanghai 200031,China ~2Department of Anatomy,School of Medicine,Waisman Centen Wicell Institute,University of Wisconsin,Madison,WI53705,USA LaboratoryofMolecularandCellBiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesGraduateSchooloftheChineseAcademyofSciences320YueyangRoadShanghai200031

Nanog is a recently discovered homeodomain transcription factor that sustains the pluripotency of embryonic stem(ES)cells and blocks their differentiation into *** murine F9 embryonal carcinoma cell line is a well-docu-mented model system for endoderm cell lineage ***,we examined the function of Nanog in F9 cellendoderm ***-expression of Nanog returns the F9 cells to the early status of ES cells and represses thedifferentiation of primitive endoderm and parietal endoderm in F9 cells,whereas it has no effect on the differentiation ofvisceral *** contrast,the expression of C-terminal domain-truncated Nanog spontaneously promotes endodermdifferentiation in F9 *** data suggest that Nanog is required to sustain the proper undifferentiated status of F9cells,and the C-terminal domain of Nanog transduces the most effects in repressing primitive endoderm and parietalendoderm differentiation in F9 cells.

关键词： Nanog F9 embryonal carcinoma ceils endoderm differentiation

Kindler syndrome protein kindlin-1 is mainly expressed in adult tissues originated from ectoderm / endoderm

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国民康医学 2015年第14期 145-145页

作者： ZHAN Jun YANG Mei ZHANG Jing GUO Yongqing LIU Wei ZHANG Hongquan Key Laboratory of Carcinogenesis and Translational Research Ministry of EducationPeking University Health Science Center State Key Laboratory of Natural and Biomimetic Drugs Peking University Health Science Center Laboratory of Molecular Cell Biology and Tumor Biology Department of AnatomyHistology and Embryology Department of Thoracic Surgery Sino - Japan Friendship Hospital Department of Immunology Third Military Medical University

Mutations of integrin-interacting protein Kindlin-1 cause Kindler Syndrome and deregulation of Kindlin-1 is implicated in human *** Kindlin-1-related diseases are confined in limited tissue ***,Kindlin-1 tissue distribution and the dogma that governs Kindlin-1 expression in normal human body are *** study examined Kindlin-1 expression in normal human adult organs,human and mouse embryonic organs by immunohistochemical *** identified a general principle that the level of Kindlin-1 expression in tissues is tightly correlated with the corresponding germ layers from which these tissues are *** compared the expression of Kindlin-1 with Kindlin-2 and found that Kindlin-1 is highly expressed in epithelial tissues derived from ectoderm and endoderm,whereas Kindlin-2 is mainly expressed in mesoderm-derived ***,Kindlin-1 was also found highly expressed in endoderm/ectoderm-derived tissues in human and mouse *** findings indicate that Kindlin-1 may play an importance role in the development of endoderm/ectoderm related tissues.

关键词： Kindlin-1 Epithelial tissue Ectoderm endoderm Embryo

Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2020年第8期12卷 580-592页

作者： Fong Cheng Pan Todd Evans Shuibing Chen Department of Surgery Weill Cornell Medical CollegeNew YorkNY 10065USA

Recent advances in development of protocols for directed differentiation from human pluripotent stem cells(hPSCs)to defined lineages,in combination with 3D organoid technology,have facilitated the generation of various endoderm-derived organoids for in vitro modeling of human gastrointestinal development and associated *** this review,we discuss current state-ofthe-art strategies for generating hPSC-derived endodermal organoids including stomach,liver,pancreatic,small intestine,and colonic *** also review the advantages of using this system to model various human diseases and evaluate the shortcomings of this ***,we emphasize how other technologies,such as genome editing and bioengineering,can be incorporated into the 3D hPSC-organoid models to generate even more robust and powerful platforms for understanding human organ development and disease modeling.

关键词： human pluripotent stem cells endoderm gastrointestinal development disease modeling

Maternal Eomesodermin regulates zygotic nodal gene expression for mesendoderm induction in zebrafish embryos

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2014年第4期8卷 272-285页

作者： Pengfei Xu Gaoyang Zhu Yixia Wang Jiawei Sun Xingfeng Liu Ye-Guang Chen Anming Meng State Key Laboratory of Biomembrane and Membrane Engineering Tsinghua- Peking Center for Life Sciences School of Life Sciences Tsinghua University Beijing 100084 China

Development of animal embryos before zygotic genome activation at the mid blastula transition （MBT） is essentially supported by eggderived maternal products. Nodal proteins are crucial signals for mesoderm and endoderm induction after the MBT. It remains unclear which maternal factors activate zygotic expression of nodal genes in the ventrotateral blastodermal margin of the zebrafish blastulas. In this study, we show that loss of maternal Eomesodermin a （Eomesa）, a T-box transcription factor, impairs zygotic expression of the nodal genes ndr1 and ndr2 as well as mesodermal and endodermal markers, indicating an involvement in mesendoderm induction. Maternal Eomesa is also required for timely zygotic expression of the transcription factor gene mxtx2, a regulator of nodal gene expression. Eomesa directly binds to the Eomes-binding sites in the promoter or enhancer of ndr1, ndr2, and rnxtx2 to activate their transcrip- tion. Furthermore, human and mouse Nodal genes are also regulated by Eomes. Transfection of zebrafish eomesa into murine embryonic stem cells promotes mesendodermal differentiation with constant higher levels of endogenous Nodal expression, suggesting a conserved function of Eomes. Taken together, our findings reveal a conserved rote of maternal T-box transcription factors in regulating nodal gene expression and mesendoderm induction in vertebrate embryos.

关键词： Eomesodermin Nodal transcription mesoderm endoderm embryo zebrafish

Tonsil-derived stem cells as a new source of adult stem cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2019年第8期11卷 506-518页

作者： Kyung-Ah Cho Hyun Jung Lee Hansaem Jeong Miri Kim Soo Yeon Jung Hae Sang Park Kyung-Ha Ryu Seung Jin Lee Byeongmoon Jeong Hyukjin Lee Han Su Kim Department of Microbiology College of Medicine Ewha Womans University Seoul 07985 South Korea Department of Chemistry and Nano Science Ewha Womans University Seoul 03760 South Korea College of Pharmacy Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul 03760 South Korea Department of Otorhinolaryngology College of Medicine Ewha Womans University Seoul 07985 South Korea Department of Otorhinolaryngology College of Medicine Hallym University Chuncheon 24252 South Korea Department of Pediatrics College of Medicine Ewha Womans University Seoul 07985 South Korea

Located near the oropharynx, the tonsils are the primary mucosal immune organ. Tonsil tissue is a promising alternative source for the high-yield isolation of adult stem cells, and recent studies have reported the identification and isolation of tonsil-derived stem cells (T-SCs) from waste surgical tissue following tonsillectomies in relatively young donors (i.e., under 10 years old). As such, TSCs offer several advantages, including superior proliferation and a shorter doubling time compared to bone marrow-derived mesenchymal stem cells (MSCs). T-SCs also exhibit multi-lineage differentiation, including mesodermal, endodermal (e.g., hepatocytes and parathyroid-like cells), and even ectodermal cells (e.g., Schwann cells). To this end, numbers of researchers have evaluated the practical use of T-SCs as an alternative source of autologous or allogenic MSCs. In this review, we summarize the details of T-SC isolation and identification and provide an overview of their application in cell therapy and regenerative medicine.

关键词： Stem cell Tonsil-derived stem cell Differentiation endoderm Mesoderm Ectoderm Cell therapy

Liver development in zebrafish (Danio rerio)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2009年第6期36卷 325-334页

作者： Ting Tao Jinrong Peng College of Animal Science Zhejiang University Hangzhou 310029 China

Liver is one of the largest internal organs in the body and its importance for metabolism, detoxification and homeostasis has been well established. In this review, we summarized recent progresses in studying liver initiation and development during embryogenesis using zebrafish as a model system. We mainly focused on topics related to the specification of hepatoblasts from endoderm, the formation and growth of liver bud, the differentiation of hepatocytes and bile duct cells from hepatoblasts, and finally the role of mesodermal signals in controlling liver development in zebrafish.

关键词： zebrafish model organism liver development endoderm

维普期刊数据库