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endocochlear potential AND POTASSIUM CONCENTRATION RECORDING IN MINIPIG COCHLEA

Journal of Otology 2012年第2期7卷 102-104页

作者： GUO Weiwei CHEN Lei LI Dengke SUN Wei YANG Shiming Department of Otolaryngology Head & Neck Surgery Institute of Otolaryngology Chinese PLA General Hospital State Key Laboratory for Agro-biotechnology China Agricultural University Center for Hearing and Deafness SUNY at Buffalo

Objective To record the endocochlear potential (EP) and calculate potassium concentration [K+] in Minipig cochleae. Methods We used multi-barreled electrodes to measure the EP and the potential, [K + ]. EP and potassium electrode recording were made in 9 cochleae from 5 minipigs to get normal EP values. Results The average EP value in the cochlea from the minipigs was 77.3 ± 14 mV. The average [K+] for the minipigs was 147.1 ± 13 mM. Conclusions The EP and potential, [K + ] in minipigs are lower than in the human and rodents. This may be the reason why porcine ABR thresholds are slightly higher than humans and rodents.

关键词： stria vascularis potassium secretion endocochlear potential minipig

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A new approach to endocochlear potential and potassium ion concentration measures in mini pig models

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Journal of Otology 2014年第4期9卷 163-168页

作者： Lili Ren Weiwei Guo Ling Zhang Wei Sun Shiming Yang Department of Otorhinolaryngology Chinese PLA General Hospital Department of Otolaryngology PLA Air Force Command College Hospital Department of Communicative Disorders & Sciences Center for Hearing and DeafnessState University of New York at Buffalo

Mini pig models are large mammals and their ears are more similar with human beings in structure and development than other animals.However,the study on porcine ears is still in the initial stage and there is no description of an ideal operation approach to endocochlear potential and potassium ion concentration measurements.In this article,we describe a pre-auricular surgical approach to access the middle and inner ear for endocochlear potential and potassium ion concentration measures in mini pig models.Ten one-week old normal mini pigs were used in the study.The bulla of the temporal bone was accessed via a pre-auricular approach for endocochlear potential and potassium ion concentration measurements.The condition of the animals during the first post—experiment 24 h was observed.One animal died during surgery.The preauricular approach improved protection and preservation of relevant nervous and vascular elements including the facial nerve and carotid artery.So,the pre-auricular approach can be used for endocochlear potential and potassium ion concentration measurements with improved nerve and artery preservation mini pigs.

关键词： Anatomy Mini pig models endocochlear potential Potassium ion concentration

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Spinster homolog 2(Spns2) deficiency causes early onset progressive hearing loss

Spinster homolog 2(Spns2) deficiency causes early onset prog...

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遗传学与表观遗传学前沿暨第三届中国青年遗传学家论坛

作者：陈静 Neil Ingham John Kelly Johanna Pass Jacqueline K White Andrew Forge Daniel Jagger Karen P Steel Wellcome Trust Sanger Institute Wolfson Centre for Age-Related Diseases King's College London Centre for Auditory Research UCL Ear Institute

Sphingosine-1-phosphate(S1P)is a biologically active lysophospholipid.Spinster homolog 2(Spns2)acts as an S1P transporter in zebrafish and mice regulating heart development and lymphocyte trafficking respectively.The mechanism of action of Spns2 is still elusive in mammals especially considering the multiple roles of S1P signalling.Here,we demonstrate that Spns2-deficient mice rapidly lost auditory sensitivity and endocochlear potential(EP)from 2 to 3weeks old.Spns2 is expressed in both the organ of Corti and lateral wall of the cochlea.We found progressive degeneration of sensory hair cells in the organ of Corti,but the earliest defect was a decline in the EP suggesting that dysfunction of the lateral wall was the primary lesion.In the lateral wall of adult mutants,we observed structural changes of marginal cell boundaries and of strial capillaries,and reduced expression of several key proteins involved in the generation of EP(Kcnj10,Kcnq1,Gjb2 and Gjb6),but these changes were likely to be secondary.Permeability of the boundaries of the striavascularis and of the strial capillaries appeared normal.Targeted inactivation of Spns2 in red blood cells,platelets,or lymphatic or vascular endothelial cells did not affect hearing,but targeted ablation of Spns2 in the cochlea using a Sox10-Cre allele produced a similar auditory phenotype to the original mutation.Our study suggests that local Spns2 expression is critical for hearing in mammals.These findings indicate that Spns2 and S1P signalling are required for normal maintenance of the EP and hence for normal auditory function.

关键词： Spns2 hearing loss mouse models endocochlear potential

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