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Determination of Melamine in Dairy Products and Melamine Tableware by Inhibition electrochemiluminescent Method

Chinese Journal of Chemistry 2011年第8期29卷 1601-1605页

作者：井伟吕水源李小晶江晓芬陈旻实梁鸣唐熙许才明陈锦权 Food Science College of Fujian Agriculture and Forestry University Fuzhou Fujian 350007 China Fujian Entry-Exit Inspection and Quarantine Bureau Key Laboratory of Consumer Products Safety Hu Dong Road 312 Fuzhou Fujian 350001 China

An electrochemiluminescent （ECL） method has been developed for the determination of melamine based on the inhibition of luminol ECL. A significant luminol ECL can be found at 1.47 V in the phosphate buffer solution at high pHs and low potential scan rates, this ECL signal can be inhibited obviously by melamine. The decrease of ECL intensity was linearly proportional to the logarithm of melamine concentration in the range of 1--100 ng/mL （R2~0.9911） and with the detection limit of 0.1 ng/mL. The method has been applied successfully to determine melamine in dairy products and melamine tableware, the recoveries were in the range of 98.5%--103.7% and 95.5%--106.0%, respectively. The mechanism of the inhibition effect was also proposed, the active oxygen （O2- ） generated from the electrooxidation of OH- reacted with luminol anion （L^-） to generate light emission, and the present of melamine can eliminate the active oxygen, which cause the decrease of the ECL intensity.

关键词： melamine luminol electrochemiluminescent inhibitor analytical method

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Development of a Multi-Plex electrochemiluminescent Assay for the Detection of Serum Antibody Responses to Meningococcal Conjugate Vaccines

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World Journal of Vaccines 2012年第1期2卷 27-35页

作者： Lani Indrawati Jon H. Heinrichs Emily P. Wen Julie M. Skinner Vaccines Research Merck Research Laboratories West Point USA

Neisseria meningitidis is a gram negative diplococcal bacterium. Worldwide, N. meningitidis is the leading cause of bacterial meningitis and sepsis, with five serogroups (A, B, C, Y, and W-135) responsible for the majority of the disease. Multivalent (A, C, Y, and W-135) polysaccharide and conjugate vaccines have been licensed in the United States and elsewhere and are widely available. We have developed a multi-plexed electrochemiluminescent assay to quantitate serum antibody responses to meningococcal polysaccharides A, C, Y, and W-135 to allow for rapid evaluation of li- censed and investigational vaccines. A 96-well plate containing a carbon electrode arrayed with polysaccharides A, C, Y, and W-135 on separate spots within each well has been developed for simultaneous detection of polysaccharidespecific antibodies in serum samples from vaccinated individuals. The assay conditions were optimized using the anti-meningococcal serogroup A/C reference serum pool, CDC 1992 (NIBSC 99/706), through evaluation of plate types, coating polysaccharide concentrations, and blocking and serum diluent buffers. Comparison of single and multiplex assays demonstrated the sensitivity, specificity, and speed of the multi-plex format for the quantification of serum antibody responses to N. meningitidis polysaccharides A, C, Y and W-135.

关键词： Neisseria meningitidis electrochemiluminescent Detection Serum Antibody

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