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Studies on electrical activation of Porcine Oocytes Matured in vitro and Embryo Culture Systems

Agricultural Sciences in China 2002年第10期1卷 1168-1173页

作者： WU Zhong-hong, XING Feng-ying, LIU Guo-shi, ZENG Shen-ming, ZHU Shi-en, ZHANG Zhong-cheng and FU Peng-hui(College of Animal Science and Technology, China Agricultural University, Beijing 100094 , P.R. China) College of Animal Science and Technology China Agricultural University Beijing 100094 P.R.China

Conditions for electrical parthenogenetic activation of porcine oocytes matured in vitro and in vitro culture systems of porcine embryo were studied. The best results were achieved under the conditions of electrical field strength and the pulse duration at 130Vmm-1/80 us, with a blastocyst development rate of (20.12 ± 8.18) % (P > 0.05). No significant difference was found between treatments of multiple pulses and a single pulse (P > 0.05). Parthenogenetic embryos were cultured with different methods and air conditions for 7 days in vitro, blastocyst development rate of embryos with changed culture media [ (26.44 ± 8.35) % ] or changed media with 10% fetal bovine serum (FBS) [ (17.68 ± 5.39)% ] on the fifth day showing no significant difference from that of embryos without change of culture media [ (25.30 ± 7.55) % , P > 0.05 ], while cell numbers of blastocysts from embryos with changed culture media (15.78 + 5.46 and 14.55 ± 4.81) were significantly lower than number of blastocysts from embryos without change of culture media (18.01 ± 6.79, P0.05]. It is concluded that change of culture media with the same new one or changing over to media with 10% fetal bovine serum (FBS) on the fifth day and low O2 environment are not necessary for porcine embryos development.

关键词： Porcine Oocytes Culture in vitro electrical activation Embryo culture

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electrical activation of Nano/Micro-size Crystallite Carbon

Electrical Activation of Nano/Micro-size Crystallite Carbon

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2011 2nd International Conference on Advances in Energy Engineering(ICAEE)

作者： Hong Guo Rui Mao Li Xu School of Chemical Science and Technology Yunnan University School of Chemistry and Chemical Engineering Qujing Normal University

Realization of electrical activation of crystallite carbon in electrochemical capacitors has been *** current charge-discharge and cyclic voltammetry were performed to characterize the electrical activation of crystallite *** showed that electrochemical capacitor could be activated deeply electrically at high voltage and obtain larger capacitance,yet unstable cycle *** activation just takes place in certain current range and the optimized current need to apply for yielding good electrical activation results with large capacitance and stable cycle *** further study,it was found that the crucial aspect impacting realization of electrical activation was electric energy.

关键词： crystallite carbon electrical activation PC-based electrolytes

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Studies on Nuclear Transplantation in Mammalian Embryos

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Journal of Northeast Agricultural University(English Edition) 1995年第1期2卷 25-34页

作者： Tan Jinghe Zhou Qi Zhang Qiuming He Guixin Liu Ruixiang Xu Libin Yang Chanyun and Qin Pengchun(Northeast Agriculture University Harbin, 150030 P R C ) Northeast Agriculture University Harbin 150030 PRC

A series of experiments were conducted to study the major procedures in nuclear transplantation such as oocyte enucleation and activation, electrofusion and developnent of the nuclear transplant embryos in the mouse, rabbits and sheep. The important results are as follows:11. In the mouse, only 35% of the oocytes collected 15～16 h after hCG had a notable first polar body (FPb) and those without FPb were enucleated by removing cytoplasm from the PVS-wider side and the enucleation rate was similar to that in the oocytes with FPb, and the enucleation rate of removing 1/3 cytoplasm was remarkably higher than that of removing 1/4 cytoplasm. 2. Among the three fusion media tested, mannitol and sucrose solutions produced better results than M2 in electrofusion of mouse 2-cell embryos. Under favorable pulse conditions, the osmotic pressure of fusion medium had no motable effect on electrofusion, but as the conditions became so unfavorable that some embryos began to lyse, the fusion rates in hypertonie mannitol solution were significantly higher than those in isotonic or hypotonic solutions. A wide range of pulse strengths (0.31～2.04 by/ cm) and durations(10～1280 μs) were used and 100% of fusion were obtained in many cases. Optimal pulse durations were plotted for field strengths to obtain high fusion rates (96%～ 100%) in mouse2-cell embryos. 3. With one pulse of 0.45 by / cm, satisfactory results of mouse oocyte activation were obtained only when the duration increased to 160 μs or longer. The activation rate increased as the oocytes got older. Some of the oocytes ar. rested at metaphase Ⅲ after electrical stimulation and their proportion to the number of oocytes not activated increased with egg age. 4. 10% and 31% of the nuclear transplant embryos developed to morula or blastocyst stage in sheep and rabbits, respectively, with Chinese-made hormones and chemicals.

关键词： nuclear transplantation electrofusion electrical activation oocyte embryo mouse rabbit sheep

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