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effect of antioxidants on endothelial cell reactive oxygen species (ROD Generation and Adhesion of Leukocytes to endothelial cells

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South China Journal of Cardiology 2000年第1期1卷 48-54页

作者：黄虔 Michael Grfe Kristoph Graf Hans Lehmkuhl Eckart Fleck Department of Cardiology Medical College of Jinan University Guangzhou 510630 PR China Department of Internal Medicine/Cardiology German Heart Institute Berlin & Virchow Hospitals Humboldt University Berlin 13353 Germany

Objective To investigatewhether antioxidants inhibit adhesion of leukocytes to endothelium and furthermore, whether all antioxidants regulate NF-KB activation through a redox sensitive mechanism. Methods The effect of the antioxidative substances pyrrolidin dithiocarbamat (PDTC), dichloroisocumarin (DCI), chrysin and probucol on the endothelial leukocyte adhesion were examined under near physiological flow conditions. The antioxidative activity of antioxidants was measured in a DCF fluorescence assay with flow cytometry. The activation of NF-kB in endothelial cells was investigated in a gel shift assay. Results PDTC and probucol did not show an inhibitory effect to the formation of intracellular H2O2 in TNFa activated human vascular endothelial cells (HUVEC) . Chrysin showed a moderate effect. DCI showed a strong antioxidative effect. In contrast, PDTC and chrysin inhibited the adhesion of HL 60 cells to TNFa-stimulated HUVEC. DCI and probucol did not have influence on the adhesion within the area of the examined shear stresses. Only PDTC inhibited the TNFa-induced activation of NF-KB in endothelial cells. Conclusion The inhibition of the endothelial leukocyte adhesion by antioxidative substances is not to be explained by its antioxidative characteristics only. The inhibitory effect of PDTC on NF-KB activation was probably not related to its antioxidative properties. endothelial cell antioxidants NF-kappa-B

关键词： HUVECs ROD Generation and Adhesion of Leukocytes to endothelial cells effect of antioxidants on endothelial cell reactive oxygen species NF DCI

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