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Multilocus enzyme electrophoresis analysis of echinostoma revolutum and echinostoma malayanum(Trematoda:echinostomatidae) isolated from Khon Kaen Province,Thailand

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Asian Pacific Journal of Tropical Medicine 2010年第8期3卷 633-636页

作者： Weerachai Saijuntha Sumonta Tapdara Chairat Tantrawatpan Walai Rukhavej Botanical Research Institute(WRBRI) Mahasarakham UniversityMahasarakhamThailand Clinical Pathology Laboratory Amnatcharoen hospitalAmnatcharoenThailand Division of Cell Biology Department of Preclinical SciencesFaculty of MedicineThammasat UniversityRangsit CampusPathumthaniThailand

Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus echinostoma,*** revolutum(***) and echinostoma malayanum(E. malayanum) from Khon Kaen Province,***:These parasites were compared at 22 enzymes encoding a presumptive 30 loci by using multilocus enzyme electrophoresis (MEE) ***:Twenty-two loci can be used as diagnostic markers to differentiate these 2 *** and *** had fixed genetic differences at 70%of loci, whereas both species had fixed genetic differences from the liver fluke,Opisthorchis viverrini at 91%of *** variation within a population of *** was observed at 5 polymorphic ***:MEE is a powerful technique to investigate genetic variation and differentiation of *** and ***.

关键词： echinostoma Echinostomes Genetic variation Multilocus enzyme electrophoresis Allozyme

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Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province,Thailand

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Asian Pacific Journal of Tropical Medicine 2014年第1期7卷 26-29页

作者： Waraporn Noikong Chalobol Wongsawad Applied Parasitology Research Laboratory Department of Biology Faculty of ScienceChiang Mai University Applied Biology Program Faculty of Science and TechnologyPibulsongkram Rajabhat University Applied Technology in Biodiversity Research Unit Institute of Science and TechnologyChiang Mai University

Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(***)and Filopaludina martensi martensi(*** martensi)and genotype variation of echinostome metacercariae by using random amplified polymorphic DNA(RAPD)***:Filopaludina *** were collected from eight localities of Lamphun Province,Northern Thailand and examined for echinostome ***-PCR was used lo analyze genotype variation of echinostome ***:A total of 3 226 *** and *** martensi snails were collected from eight *** overall prevalences of echinostome metacercariae in *** and *** martensi were 40.89%and 36.27%,while the intensity of infection was 20.37 and 12.04,*** dendrogram constructed base on RAPD profiles,4 well supported domains were generated;(i)group of metacercariae from Ban Hong,Mae Ta,Meaung,Pa Sang,Toong Hua Chang,and Weang Nong that were clustered in the group of ***,(ii)Ban Thi,(iii)Lee,and(iv)3 adults of an out ***:This research demonstrated RAPD profiling has been a useful tool to detect DNA polymorphisms to determine genetic relationship between echinostome metacercariae in Lamphun Province,Northern Thailand.

关键词： echinostoma sp. Filopaludina sp.snails RAPD-PCR Epidemiology Metacercariae

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