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Differences in carbon source usage by dental plaque in children with and without early childhood caries

International Journal of Oral Science 2017年第4期9卷 232-237页

作者： yan zhao wen-jie zhong zhe xun qian zhang ye-qing song yun-song liu feng chen the second dental center peking university school and hospital of stomatologyBeijingChina central laboratory peking university school and hospital of stomatologynational engineering laboratory for digital and material technology of stomatologybeijing key laboratory of digital stomatologyBeijingChina department of prosthodontics peking university school and hospital of stomatologyBeijingChina

early childhood caries （ECC） is a considerable pediatric and public health problem worldwide. Preceding studies have focused primarily on bacterial diversity at the taxonomic level. Although these studies have provided significant information regarding the connection between dental caries and oral micmbiomes, further comprehension of this microbial community＇s ecological relevance is limited. This study identified the carbon source metabolic differences in dental plaque between children with and without ECC. We compared the microbial community functional diversity in 18 caries-free subjects with 18 severe ECC patients based on sole carbon source usage using a Biolog assay. The anaerobic microbial community in the ECC patients displayed greater metabolic activity than that of the control group. Specific carbon source metabolism differed significantly between the two groups. Subjects from the two groups were well distinguished by cluster and principal component analyses based on discriminative carbon sources. Our results implied that the microbial functional diversity between the ECC patients and healthy subjects differed significantly. In addition, the Biolog assay furthered our understanding of oral microbiomes as a composite of functional abilities, thus enabling us to identify the ecologically relevant functional differences among oral microbial communities.

关键词： Biolog assay carbon source utilization early childhood caries microbial community

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Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment

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International Journal of Oral Science 2017年第4期9卷 185-192页

作者： abdullah s hemadi rui-jie huang yuan zhou jing zou state key laboratory of oral diseases&national clinical research center for oral diseases&department of pediatric dentistry west china hospital of stomatologysichuan universityChengduChina

early childhood caries （ECC） is a term used to describe dental caries in children aged 6 years or younger. Oral streptococci, such as Streptococcus mutans and Streptococcus sorbrinus, are considered to be the main etiological agents of tooth decay in children. Other bacteria, such as Prevotella spp. and Lactobacillus spp., and fungus, that is, Candida albicans, are related to the development and progression of ECC. Biomolecules in saliva, mainly proteins, affect the survival of oral microorganisms by multiple innate defensive mechanisms, thus modulating the oral microflora. Therefore, the protein composition of saliva can be a sensitive indicator for dental health. Resistance or susceptibility to caries may be significantly correlated with alterations in salivary protein components. Some oral microorganisms and saliva proteins may serve as useful biomarkers in predicting the risk and prognosis of caries. Current research has generated abundant information that contributes to a better understanding of the roles of microorganisms and salivary proteins in ECC occurrence and prevention. This review summarizes the microorganisms that cause caries and tooth-protective salivary proteins with their potential as functional biomarkers for ECC risk assessment. The identification of biomarkers for children at high risk of ECC is not only critical for early diagnosis but also important for preventing and treating the disease.

关键词： caries risk assessment early childhood caries salivary microorganisms salivary proteins

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Iron deficiency and early childhood caries:a systematic review and meta-analysis

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Chinese Medical Journal 2021年第23期134卷 2832-2837页

作者： Shuai-Qi Ji Rui Han Ping-Ping Huang Shuang-Yi Wang Hao Lin Lei Ma School of Stomatology of Qingdao University QingdaoShandong 266000China Department of Stomatology Affiliated Hospital of Qingdao UniversityQingdaoShandong 266000China

Backgrounds:Previous surveys have found that children with iron deficiency(ID)were likely to suffer from early childhood caries(ECC).We aimed to assess the scientific evidence about whether ID is intrinsically related to ***:The medical subject headings(MeSH)terms and free words were searched on PubMed,Web of Science,Cochrane,China National Knowledge Infrastructure,Wanfang,and the Database for Chinese Technical Periodicals from March 2020 to September *** researchers independently screened the *** extraction and cross-checking were performed for the studies that met the inclusion ***-analysis was performed using the Cochrane Collaboration’s Review Manager 5.3 ***:After excluding duplication and irrelevant literature,12 case-control studies were included in the *** meta-analysis demonstrated that children with ECC were more likely to have ID(odds ratio[OR]=2.63,95%confidence interval[CI]:[1.85,3.73],P<0.001).There was no statistically significant association found between the level of serum ferritin and ECC(weighted mean difference(WMD)=5.80,95%CI:[11.97,0.37],P=0.07).Children with ECC were more likely to have iron-deficiency anemia(OR=2.74,95%CI:[2.41,3.11],P<0.001).The hemoglobin(HGB)levels in the ECC group were significantly lower compared with that in the ECC-free group(WMD=9.96,95%CI:[15.45,4.46],P=0.0004).The mean corpuscular volume(MCV)levels in the ECC group were significantly lower compared with that in the ECC-free group(WMD=3.72,95%CI:[6.65,0.79],P=0.01).Conclusions:ID was more prevalent in children with ECC,and the markers of iron status in the ECC group,such as serum ferritin,HGB,and MCV,were relatively lower than the ECC-free group.

关键词： Iron deficiency Iron deficiency anemia early childhood caries

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Characterizing Diversity of Lactobacilli Associated with Severe early childhood caries: A Study Protocol

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Advances in Microbiology 2015年第1期5卷 9-20页

作者： Yihong Li Silvia Argimón Catherine N.Schon Prakaimuk Saraithong Page W.Caufield Department of Basic Science and Craniofacial Biology College of DentistryNew York UniversityNew YorkUSA Department of Cariology and Comprehensive Care College of DentistryNew York UniversityNew YorkUSA

Lactobacilli have been consistently associated with dental caries for decades;however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit identification of oral Lactobacillus species is possible, despite their taxonomic complexity. Here we describe a combined approach involving both cultivation and genetic methods to ascertain and characterize the diversity and abundance of the Lactobacillus population in the oral cavities of children with severe early childhood caries (S-ECC). Eighty 3- to 6-year-old children (40 S-ECC and 40 caries free) who were seeking dental care at the Pediatric Dental Clinic of Bellevue Hospital in New York City were invited to participate in this study. Clinical data on sociodemographic information and oral health behavior were obtained from the primary caregiver. The data included a detailed dental examination, children’s medical history, and a questionnaire survey. Combined non-stimulated saliva and supragingival plaque samples were collected from each child and cultivated on selective media for quantitative measures of lactobacilli levels. The procedure for Lactobacillus species screening will include the random selection of 50 colonies per plate, ex- traction of DNA from each colony, and genotyping by arbitrarily primed polymerase chain reaction (AP-PCR). Each unique Lactobacillus AP-PCR genotype will be selected for taxonomic assessment by 16S rRNA gene sequencing analysis. Lactobacillus species will be identified by comparing the 16S rRNA sequences with the Ribosomal Database and the Human Oral Microbiome Database. Meanwhile, the same set of clinical samples will be independently subjected to genomic DNA isolation, 16S rRNA amplification with Lactobacillus genus-specific primers, sequencing, and taxonomic identification, both at genus and species levels with a customized pipeline. The distribution and phylogenetic differences of these Lactobacillus species will

关键词： Lactobacilli early childhood caries Bacterial Diversity 16S rRNA AP-PCR Saliva Dental Plaque

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A Survey on the Prevalence of early childhood caries and Its Impact on Child Oral Health-related Quality of Life of 3-4 years old Children in Conghua

A Survey on the Prevalence of Early Childhood Caries and Its...

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中华口腔医学会第14次全国口腔医学学术会议（2012年...

作者： Guanghua School of Stomatology,Sun Yat-sen University,Guangzhou,Guangdong Province(510055) Limuyue

Objectives The objectives of this survey were to explore the early childhood caries(ECC) among 3-4 years old children in Conghua of Guangzhou and explore the possible relationship between child oral health-related quality of life and ECC. Methods This study followed a cross-sectional design,with a random cluster sample of 1062 schoolchildren aged 3-4 years,representative of Conghua of *** completed the Chinese version of the early childhood Oral Health Impact Scale (ECOHIS),their parents answered questions on demographic and socioeconomic status,and a dental examination provided information on the prevalence of *** univariate analyses of t-test,One-Way ANOVA,Spearman correlate analysis and multiple analysis of ANCOVA were used. Results 510 of the 1062 participants had ECC,while others were *** prevalence of ECC was 47.6%,with a mean dmft(decayed-missing-filled tooth)score at 1.9 or a mean dmfs(decayed-missing-filled tooth surface)score at *** ft (filled tooth)only made up 7.8%of the dft(decayed-filled tooth)*** most prevalent teeth of ECC were maxillary primary incisors,maxillary and mandibular primary *** most prevalent surfaces were proximal and occlusal surfaces. The total ECOHIS scores,from the scale sets returned,are 0-31,and their average scores are 3.1±*** the results of the reliability analysis of the scale,the Cronbach’s a,the internal consistency reliability,is *** the results of the validity analysis,the correlation coefficients between items and the scale are *** results of the final multiple analysis of ANCOVA showed that the ECOHIS score and subscale scores correlated strongly with the dmfs indices. Demographic and socioeconomic factors were not associated with COHRQoL. Conclusions 1:The prevalence of ECC in 3-4 years old children in Conghua of Guangzhou was 47.6%and mean dmft was *** ratio of ft/dft was low and treatment need is huge. 2:The Chinese version of the ECOHIS

关键词： early childhood caries child oral health-related quality of life cross-sectional survey

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Bioresponsive nanotherapy for preventing dental caries by inhibiting multispecies cariogenic biofilms

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Bioactive Materials 2022年第8期7卷 1-14页

作者： Danfeng Liu Xianbin Ma Yaoting Ji Rourong Chen Shuhui Zhou Hantao Yao Zichen Zhang Mengjie Ye Zhigang Xu Minquan Du The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)and Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhan430079PR China School of Materials and Energy&Chongqing Engineering Research Center for MicroNano Biomedical Materials and Devices Southwest UniversityChongqing400715PR China

early childhood caries(ECC)is a public healthcare concern that greatly reduces the quality of life of young *** a leading factor of ECC,cariogenic biofilms are composed of acidogenic/aciduric pathogens and extracellular polysaccharides(EPSs),creating an acidic and protected *** photodynamic therapy(aPDT)is a noninvasive,painless,and efficient therapeutic approach that is suitable for treating ***,due to the hyperfine structure of cariogenic biofilms,most photosensitizers(PSs)could not access and penetrate deeply in biofilms,which dramatically hamper their efficiency in the ***,bioresponsive nanoparticle loaded with chlorin e6(MPP-Ce6)is developed,which largely increases the penetration depth(by over 75%)and retention(by over 100%)of PS in the biofilm compared with free ***,MPP-Ce6-mediated aPDT not only kills the bacteria in preformed biofilms but also inhibits multispecies biofilm formation.A rampant caries model is established to mimic ECC in vivo,where the population of cariogenic bacteria is decreased to 10%after MPP-Ce6-mediated ***,the number and severity of carious lesions are efficiently reduced via Keyes’scoring and micro-CT *** simple but effective strategy can serve as a promising approach for daily oral hygiene in preventing ECC.

关键词： early childhood caries Antimicrobial photodynamic therapy Multispecies biofilms Bioresponse Nanotherapy

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