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饮水型砷暴露人群 erβ mRNA 的表达及意义

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卫生研究 2014年第3期43卷 472-476页

作者：韩晓红郭志伟杨卫红武克恭李艳红夏雅娟内蒙古自治区地方病防治研究中心呼和浩特010031 山东省德州市疾病预防控制中心

目的测定不同饮水型砷暴露人群血液雌激素受体β(erβ)mRNA表达水平,分析erβmRNA表达水平与砷致心脏损伤的关系,进一步探讨砷的内分泌干扰效应。方法采用分子流行病学调查方法,在饮水型地方性砷中毒病区选择调查对象273人,依据饮水砷... 详细信息

目的测定不同饮水型砷暴露人群血液雌激素受体β(erβ)mRNA表达水平,分析erβmRNA表达水平与砷致心脏损伤的关系,进一步探讨砷的内分泌干扰效应。方法采用分子流行病学调查方法,在饮水型地方性砷中毒病区选择调查对象273人,依据饮水砷浓度分为高、中、低和对照4个剂量组,运用实时荧光定量RT-PCR技术,检测其血液erβmRNA表达水平。结果人群erβmRNA表达量随着饮用水砷浓度及尿砷含量的增加而上升(r分别为0.159和0.21,PerβmRNA表达水平与Q-Tc间期延长患病率变化趋势一致;Tp-Te间期随水砷浓度的增加而延长(r=0.199,P=0.023),同样也随erβmRNA表达的增加而延长(r=0.205,P=0.019);各组心律失常患病率几乎与erβmRNA表达水平变化趋势同步。结论长期砷暴露可潜在地影响人体erβ基因表达,砷致Q-Tc间期和Tp-Te间期延长与干扰erβ基因表达可能有密切联系。

关键词：砷 erβ mRNA 心脏损伤

erβ表达载体的构建及其在肿瘤细胞中的功能研究

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中华肿瘤杂志 2003年第4期25卷 340-343页

作者：朱建华叶棋浓江泽飞钟红君严景华吕秋军宋三泰黄翠芬军事医学科学院生物工程研究所北京*** 北京军事医学科学院第三○七医院乳癌内科北京军事医学科学院放射医学研究所

目的　构建erβ表达载体 ,探讨其在肿瘤细胞株中的表达及功能。方法　利用常规PCR技术扩增人全长erβ编码序列 ,并将其克隆到真核表达载体pCDNA3中 ,得到重组质粒pCDNA3 erβ。用Westernblot和体外翻译方法检测erβ的表达情况。将pCDNA... 详细信息

目的　构建erβ表达载体 ,探讨其在肿瘤细胞株中的表达及功能。方法　利用常规PCR技术扩增人全长erβ编码序列 ,并将其克隆到真核表达载体pCDNA3中 ,得到重组质粒pCDNA3 erβ。用Westernblot和体外翻译方法检测erβ的表达情况。将pCDNA3 erβ分别转染SV4 0病毒转化的胚胎肾细胞 2 93T、乳腺癌细胞MDA MB 4 35、MDA MB 4 36、SKBR3和前列腺癌细胞PC 3,用含雌激素应答元件的报告基因系统检测erβ在这些不同细胞中的功能。结果　经酶切鉴定 ,证实重组质粒pCDNA3 erβ含有目的基因erβ。Westernblot检测表明 ,转染pCDNA3 erβ的细胞表达了相对分子量为 6 30 0 0的erβ蛋白。体外翻译进一步证实该表达载体翻译了erβ蛋白。报告基因系统检测表明 ,erβ在不同肿瘤细胞中的表达均激活了雌激素应答的erE和C3报告基因的表达。结论　pCDNA3 erβ在肿瘤细胞中既可表达 ,又具有功能活性 ,为进一步研究erβ在肿瘤细胞中的作用奠定了基础。

关键词：肿瘤 erβ 雌激素受体基因转染

人雌激素受体erα/erβ在大肠杆菌中的表达

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药学学报 2012年第10期47卷 1399-1402页

作者：罗璐赵淑娟王峥涛胡之璧上海中医药大学中药研究所国家中医药管理局中药新资源和品质评价重点研究室上海复方中药重点实验室上海201203

雌激素主要通过与雌激素受体（estrogen receptor,er）结合而发挥作用,er是一类有配体激活的核转录因子。er分为erα和erβ两种亚型,它们在人体内具体表达部位不同,发挥的功能也有所不同。

关键词：雌激素受体重组蛋白表达 erα erβ

金雀黄素对子宫内膜癌细胞erα和erβmRNA水平的调节

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北京大学学报（医学版） 2005年第3期37卷 278-280页

作者：薛晓鸥魏丽惠北京大学人民医院妇科北京100044

目的:研究金雀黄素对子宫内膜癌细胞erα,erβmRNA的调节作用,探讨植物雌激素对子宫内膜癌的作用机制,为临床应用植物雌激素提供理论依据。方法:体外培养子宫内膜癌细胞系Ishikawa,采用实时荧光定量RT PCR方法,观察不同剂量金雀黄素对... 详细信息

目的:研究金雀黄素对子宫内膜癌细胞erα,erβmRNA的调节作用,探讨植物雌激素对子宫内膜癌的作用机制,为临床应用植物雌激素提供理论依据。方法:体外培养子宫内膜癌细胞系Ishikawa,采用实时荧光定量RT PCR方法,观察不同剂量金雀黄素对两种雌激素亚型表达的影响,阳性对照为雌二醇、孕酮作用组。结果: (1)低剂量金雀黄素能提高子宫内膜癌细胞erαmRNA表达,但其作用较雌二醇明显弱;低剂量金雀黄素能降低erβmRNA的表达,而雌二醇对erβmRNA作用不明显。(2)高剂量金雀黄素的作用与孕酮相似,能下调子宫内膜癌细胞erαmRNA和erβmRNA表达,但较孕酮作用弱。结论:金雀黄素对子宫内膜癌细胞erαmRNA和erβmRNA的表达作用不同,而且与其剂量有一定关系。推测金雀黄素有雌激素受体调节剂的作用。

关键词：金雀黄素 erβ mRNA水平 erα 定量RT-PCR方法雌激素受体调节剂 mRNA表达植物雌激素 αmRNA 子宫内膜癌雌二醇调节作用作用机制临床应用癌细胞系体外培养实时荧光亚型表达不同剂量阳性对照表达作用低剂量

erβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway

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ERβ modulates genistein's cisplatin-enhancing activities in...

Estrogen suppressed HCC progression through erβ-mediated upregulation of NLRP3 inflammasome

华东六省一市生物化学与分子生物学会联盟2019年学术与教学交流会

作者： Ren Liu Xiaolin Xu Chenglin Liang Xiaojuan Hu Department of Biochemistry and Molecular Biology School of Basic Medical ScienceNanchang University

As one of the typical food-derived phytoestrogens,genistein(GEN) could bind to estrogen receptor(er) and was reported to be closely related to breast *** former research showed that GEN interfered with the anti-tumor effects of cisplatin(CIS) in breast cancer MCF-7(era+/erβ-) ***,it is not clear whether er expression pattern affects GEN’s modulation on CIS’s *** the present study,breast cancer erP knockdown(erβKD) MDA-MB-231(erα-/erβ+) cell model was established via erβ RNAi lentivirus *** role of erβ expression in GEN’s bioeffects on cells,response to CIS was investigated and was further double-checked by pathway-specific inhibitor *** results were harvested through cell viability analysis,cell cycle distribution flow cytometry,TUNEL staining,and expression detection of key biomarkers,Bax,Bcl-2,P21,P53,and cleaved *** with the control group,PHTPP-treated or erβKD cells exhibited higher sensitivity to both GEN and CIS *** and CIS showed synergistic effects only in erβ-deficient *** effect mainly resulted in G2 phase arresting and apoptosis induction with the upregulation of P21 and Bax/Bcl-2 protein ***,P53 expression was strikingly suppressed in erβ-deficient *** indicated erP pathway deficiency might enhance GEN-CIS bioactivity via the downregulation of *** summary,our data imply that daily intake of GEN-rich diet could collaborate with CIS anti-tumor treatment in erα-/erβ-breast cancer cases,erβ pathway might be one of the potential targets which elicit GEN’s positive effects in era-breast cancer patients.

关键词： Genistein Cisplatin Breast cancer erβ Chemotherapy P53-independent

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Estrogen suppressed HCC progression through ERβ-mediated up...

PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the erα/erβ protein ratio

第九届全国免疫学学术大会

作者： Qing Wei Pengbo Guo Kun Mu Ying Zhang Wanwan Huai Wei Zhao Lihui Han Department of Immunology Shandong University School of Medicine

Background:Hepatocellular carcinoma(HCC)is one of the most common malignancies *** incidence of HCC is strikingly higher in male than in female,and estrogen has recently emerged as a protective factor in the development of HCC though its mechanism is not *** previous work found that expression of NLRP3 inflammasome components was significantly down-regulated in HCC tissues compared with the adjacent non-tumor tissues,and has proved that deregulation of NLRP3 inflammasome was involved in HCC *** the mechanism of how it was deregulated was not *** on the fact of gender disparity in HCC patients and the reports showing regulation of some inflammasome components by estrogen,we are interested to know whether there is any potential link among estrogen and NLRP3 inflammasome in HCC ***:This study was aimed to determine the expression of estrogen receptor on HCC tissues and the effect of estrogen on the malignant behaviors of HCC *** particular,this work is to determine whether expression and activation of NLRP3 inflammasome could be regulated by E2/erβpathway and how it exerted an anti-oncogenic effect of HCC ***:128 paired HCC tissues and the adjacent non-cancerous liver tissues were used for the tissue microarray(TMA)construction,and erβexpression were measured by *** analysis was performed to detect relationship between erβand NLRP3 inflammasome ***-PCR,real-time PCR and western blot were performed to determine the effect of E2 on the expression of NLRP3 inflammasome ***-8 assay,colony formation assay,LDH release assay and cell migration assay were all used to analyze the malignant behaviors of HCC ***:This study showed that expression of estrogen receptor(er)βwas significantly downregulated in HCC tissues compared with that in non-cancerous liver tissues,and its expression level was significantly negatively correlated with disease progression and positively corre

关键词： HCC NLRP3 inflammasome estrogen erβ

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PES1 promotes the occurrence and development of papillary th...

erβ相互作用蛋白PSMC5的分离及其在erβ信号途径中的作用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Yi-Bo Qiu Ling-Yao Liao Rong Jiang Man Xu Lin-Wan Xu George G.Chen Zhi-Min Liu Department of Biochemistry and Molecular Biology Chongqing Medical University Department of Pathology Molecular Medicine and Cancer Research CenterChongqing Medical University Department of Surgery Chinese University of Hong KongPrince of Wales Hospital

Estrogen has been suggested to play a role in the occurrence and development of papillary thyroid cancer(PTC).The balance and the ratio between erα and erβ protein are crucial in determining the effect of estrogen on target tissues and ***1,a BRCT domain-containing protein,has been shown to play a role in modulating the balance and the ratio between erα and erβ protein,which is associated with the occurrence and development of breast and ovarian ***,the role of PES1 in connection with the balance and the ratio between erα and erβ protein in the occurrence and development of PTC remains *** this study,PES1 protein level in human PTC tissues and cells was examined using immunohistochemistry and Western blotting,*** correlations of PES1 protein level with erα and erβ protein levels,with various clinicopathological features of PTC patients,with the erα/erβ protein ratio and the proliferation,invasion and migration of PTC cells were assessed using statistical *** results showed that erα and erβ were co-expressed in human PTC tissues and ***α promoted and erβ inhibited the proliferation,invasion and migration of PTC cells and a Yin-Yang balance existed between erα and erβ.PES1 modulated this Yin-Yang balance by elevating erα and reducing erβ protein level,then upregulating the erα/erβ protein ratio and promoting the proliferation,invasion and migration of PTC *** PTC tissues,PES1 protein level positively correlated with erα(rs = 0.549,P erβ protein level(rs =-0.353,P erα protein levels were gradually increased and erβ protein level was decreased by degree in the occurrence and development of *** PES1 and erα and decreased erβ protein levels correlated with aggressive PTC behaviors such as large tumor size,extrathyroidal extension(ETE),lymph node metastasis(LNM) and high TNM *** was suggested that PES1 promotes the occurrence and development of P

关键词： PES1 erα erβ papillary thyroid cancer

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中国生物化学与分子生物学报 2006年第5期22卷 384-389页

作者：王晓辉张浩周蕾丁丽华韩聚强宁康孙琰袁斌刘辉常庆李杰之叶棋浓军事医学科学院生物工程研究所北京100850

雌激素受体β(erβ)在乳腺癌发生发展中起着重要的作用,寻找与erβ相互作用的共调节因子对阐明er信号通路具有重要价值.应用酵母双杂交技术,以erβ的AF2结构域为诱饵蛋白从人乳腺文库中筛选出了与之相互作用的蛋白26S蛋白酶的亚单位ATPa... 详细信息

雌激素受体β(erβ)在乳腺癌发生发展中起着重要的作用,寻找与erβ相互作用的共调节因子对阐明er信号通路具有重要价值.应用酵母双杂交技术,以erβ的AF2结构域为诱饵蛋白从人乳腺文库中筛选出了与之相互作用的蛋白26S蛋白酶的亚单位ATPase5(PSMC5).GST沉淀实验表明,PSMC5在体外特异地与erβ相结合.转录活性实验表明,PSMC5以激素依赖的方式降低erβ转录活性.上述结果提示,PSMC5可能通过影响erβ信号途径在乳腺癌发生发展中起着重要的作用.

关键词： erβ PSMC5 相互作用共调节因子

er拮抗剂对人子宫内膜样癌JEC细胞中erα、erβ、p57^(kip2)表达的影响

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临床与实验病理学杂志 2018年第3期34卷 288-294页

作者：刘蒙蒙袁丹周正平孙小杰刘俊江遵义医学院病理学教研室遵义563003 滨州市人民医院病理科滨州256610 遵义医学院基础医学院电镜室遵义563003

目的探讨雌激素受体(estrogen receptor,er)拮抗剂对人子宫内膜样癌(endometrioid carcinoma,EC)JEC细胞(中分化)中er亚型erα、erβ及p57^(kip2)表达的影响。方法分别用两种er拮抗剂[他莫昔芬(Tamoxifen,TAM)(10^(-6)mol/L)、氟维司群(Faslodex,ICI182780)(10^(-6)mol/L)]及雌二醇(β-Estradiol,E_2)(10^(-6)mol/L)干扰JEC细胞,体外培养24、48、72 h后,MTT法观察JEC细胞的生长曲线;光镜与电镜观察JEC细胞的形态变化;Western blot法检测JEC细胞中erα、erβ及p57^(kip2)蛋白表达的变化。结果 MTT结果显示,与对照组JEC细胞比较,E_2可明显促进JEC细胞增殖,ICI182780则明显抑制JEC细胞的增殖(Pern blot结果显示:与对照组比较,E_2组erβ蛋白表达增高,p57^(kip2)蛋白表达降低(Perβ蛋白表达均降低,p57^(kip2)蛋白表达均增高,但仅ICI182780组差异有统计学意义(Perβ蛋白表达均降低,p57^(kip2)蛋白表达均增高,但仅E_2+ICI182780组差异有统计学意义(Perα蛋白表达。结论 JEC细胞不表达erα蛋白。ICI182780有较强的拮抗雌激素的作用,可能通过下调JEC细胞中erβ蛋白表达,诱导p57^(kip2)蛋白的表达,发挥其阻滞细胞周期进程的作用,从而抑制肿瘤细胞生长;TAM则对JEC细胞生长有较弱的雌激素样作用。联合检测erα、erβ、p57^(kip2)蛋白在EC中的表达,对EC患者内分泌治疗的个体化选择有重要的参考价值。

关键词：子宫内膜癌雌激素受体拮抗剂 erα erβ p57kip2 TAM ICI182780