检索结果-南通市图书馆

Non-SMC elements 1 and 3 are required for early embryo and seedling development in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Non-SMC elements 1 and 3 are required for early embryo and s...

Partridge embryo pathology in relation to gentamicin-induced lesions

2016年全国植物生物学大会

作者： Gang Li Wenxuan Zou Liufang Jian Jie Qian Yingtian Deng Jie Zhao State Key Laboratory of Hybrid Rice College of Life SciencesWuhan University

The structural maintenance of the chromosome 5/6(SMC5/6) complex has a variety of functions in mitosis and meiosis in yeast and human,including facilitating homologous recombination,restarting stalled replication forks,and maintaining ribosomal DNA and heterochromatin,among other roles. In Arabidopsis,At SMC5 and At SMC6A/6B are responsive to DNA damage. However,the non-SMC element 1 and 3 functions in Arabidopsis growth and development remain as yet unknown. In our study,it was found that the loss function of the non-SMC element 1 and 3 led to the conversion of suspensor cell fate and caused defects in embryo pattern formation. Partially-complemented homologous mutants showed that the post-embryonic development of the mutants was inhibited,that chromosome fragments occurred during segregation of chromosomes,and that the transition from the G2 phase to the M phase was delayed,leading to endoreduplication. All of these results demonstrate that the non-SMC element 1 and 3 are essential for cell division and function in maintaining the stability of chromosome ploidy in mitosis. Further,a large number of dead cells and DNA DSBs were observed in the their mutants,and the expression of the non-SMC element 1 and 3 were up-regulated following treatment of the plants with DSBs inducer compounds,indicating that the non-SMC element 1 and 3 play roles in DNA damage repair. Therefore,we conclude that the nonSMC element 1 and 3 facilitate DSBs repair and contribute to maintaining the stability of chromosome ploidy in mitotic cells. The non-SMC element 1 and 3 could thus be considered as required factors for maintaining proper early embryo cell differentiation and post-embryonic development.

关键词： non-SMC elements embryo seedling Arabidopsis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Journal of Coastal Life Medicine 2016年第10期4卷 833-837页

作者： Hadi Tavakkoli Amin Derakhshanfar Department of Avian Medicine School of Veterinary MedicineShahid Bahonar University of KermanKermanIran Diagnostic Laboratory Sciences and Technology Research Center Center of Comparative and Experimental MedicineShiraz University of Medical SciencesShirazIran

Objective:To determine the macroscopic and microscopic lesions of various dosages of gentamicin in the partridge ***:Fertile chukar partridge eggs were allocated into four *** 1:saline-injected group whose individuals were administered by sterile physiological saline solution of 0.2 mL/egg inserted into yolk *** 2,3 and 4 whose individuals were similarly administered by gentamicin sulfate at a dosage of 80 mg/kg egg-weight once,twice and three times,***:Results showed that the embryos were congested and stunted in the gentamicininjected *** in feet,wings and feather development were accompanied by microscopic lesions in brain,meninges,heart,lungs,liver and *** lesions were noticed as edema,undeveloped tissues,necrosis and degeneration in the affected ***:Based on acquired results,it is concluded that gentamicin at above-described dosages causes toxicopathological effects to the partridge embryo in a dose dependent manner.

关键词： Aminoglycosides embryo Gentamicin Pathology

Role of Oct4 in the early embryo development

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Regeneration 2014年第1期3卷 50-59页

作者： Guangming Wu Hans R Schöler Max Planck Institute for Molecular Biomedicine Department of Cell and Developmental BiologyRöntgenstrasse 2048149 MünsterGermany University of Münster Medical FacultyDomagkstr.348149 MünsterGermany

Oct4 is a key component of the pluripotency regulatory network,and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells(ESCs)or their differentiation into ***4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic ***,the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected,and that these embryos could complete full-term development without any obvious *** results indicate that Oct4 is not essential for the initiation of pluripotency,in contrast to its critical role in maintaining *** conclusion is further supported by the formation of Oct4-GFP–and Nanog-expressing inner cell masses(ICMs)in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes.

关键词： Oct4 Oct4B Totipotency Pluripotency embryo Development

embryo Glue和G2两种移植液对冻融卵裂胚移植结局的影响比较

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

吉林医学 2019年第8期40卷 1689-1690页

作者：刘利敏陈志云惠州市中心人民医院生殖医学中心

目的:探讨embryo Glue移植液和G2两种移植液对冻融卵裂胚移植的临床妊娠率、早期流产率、异位妊娠率的影响。方法:回顾性分析2015年1月~2017年5月底期间1 068例以embryo Glue和G2为移植液的冻融卵裂胚的临床妊娠率、早期流产率、异位妊... 详细信息

目的:探讨embryo Glue移植液和G2两种移植液对冻融卵裂胚移植的临床妊娠率、早期流产率、异位妊娠率的影响。方法:回顾性分析2015年1月~2017年5月底期间1 068例以embryo Glue和G2为移植液的冻融卵裂胚的临床妊娠率、早期流产率、异位妊娠率。结果:以G2和embryo Glue为移植液的1 068例冻融卵裂胚的临床妊娠率、早期流产率、异位妊娠率分别为50.99%vs 47.96%、10.34%vs10.88%、3.45%vs2.38%,差异无统计学意义(P>0.05)。结论:embryo Glue移植液不能提高冻融周期的临床妊娠率、降低流产率,不能提高宫内妊娠率。

关键词： embryo Glue G2 临床妊娠率早期流产率妊娠结局

Three-dimensional spatial transcriptome landscape for early mouse embryo

在线全文

学校读者我要写书评

暂无评论

Science Foundation in China 2016年第3期24卷 13-13页

The research team led by *** Naihe(景乃禾)from the Institute of Biochemistry and Cell Biology and Han Jingdong(韩敬东)from the CAS-MPG Partner Institute for Computational Biology,Shanghai Institutes for Biological Sci... 详细信息

关键词： landscape embryo 韩敬东 conserved diverse vertebrate profiling spatially specification capture

High-efficiency embryo Cryopreservation Technology for Mutton Sheep

在线全文

学校读者我要写书评

暂无评论

Agricultural Biotechnology 2023年第2期12卷 45-47页

作者： Caihong HAO Guizhi TONG Xueying SONG Xintao LI Likun WANG Zhenhua JIN Jiahou WANG Wenkai GUO Ping LI Li LI Yan ZHANG Animal Husbandry and Veterinary Branch Heilongjiang Academy of Agricultural SciencesQiqihar 161005China Institute of Animal Biotechnology Jilin Academy of Agricultural SciencesChangchun 130000China

embryo cryopreservation is an important part of embryo *** plays a key role in the development of quality animal ***,the effect of in-vivo(in-vitro)embryo freezing of cattle and sheep in China has not reached an expected level,which limits the commercial application of embryo transfer *** technical bottleneck that needs to be broken through is that embryos are subject to mechanical damage and protective agent toxicity during freezing and *** paper summarized the successful application of plant antifreeze protein in vitrification preservation of pig embryos,which makes this technology widely used in animal husbandry production,and is of great significance in accelerating the large-scale breeding of improved livestock and saving endangered animals.

关键词： Sheep Mutton sheep embryo Freezing

embryonic thermal manipulation:a potential strategy to mitigate heat stress in broiler chickens for sustainable poultry production

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第4期15卷 1347-1375页

作者： Sadid Al Amaz Birendra Mishra Department of Human Nutrition Food and Animal SciencesCollege of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaAgSci 2161955 East‑West RdHonoluluHI 96822USA

Due to high environmental temperatures and climate change, heat stress is a severe concern for poultry health and production, increasing the propensity for food insecurity. With climate change causing higher temperatures and erratic weather patterns in recent years, poultry are increasingly vulnerable to this environmental stressor. To mitigate heat stress, nutritional, genetic, and managerial strategies have been implemented with some success. However, these strategies did not adequately and sustainably reduce the heat stress. Therefore, it is crucial to take proactive measures to mitigate the effects of heat stress on poultry, ensuring optimal production and promoting poultry well-being. embryonic thermal manipulation(TM) involves manipulating the embryonic environment's temperature to enhance broilers' thermotolerance and growth performance. One of the most significant benefits of this approach is its cost-effectiveness and saving time associated with traditional management practices. Given its numerous advantages, embryonic TM is a promising strategy for enhancing broiler production and profitability in the poultry industry. TM increases the standard incubation temperature in the mid or late embryonic stage to induce epigenetic thermal adaption and embryonic metabolism. Therefore, this review aims to summarize the available literature and scientific evidence of the beneficial effect of pre-hatch thermal manipulation on broiler health and performance.

关键词： Broiler embryo Epigenetics Global warming Thermoregulation

Knowledge-embedded spatio-temporal analysis for euploidy embryos identification in couples with chromosomal rearrangements

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2024年第6期137卷 694-703页

作者： Fangying Chen Xiang Xie Du Cai Pengxiang Yan Chenhui Ding Yangxing Wen Yanwen Xu Feng Gao Canquan Zhou Guanbin Li Qingyun Mai Reproductive Medicine Center The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510080China Guangdong Provincial Key Laboratory of Reproductive Medicine GuangzhouGuangdong 510080China School of Computer Science and Engineering Sun Yat-sen UniversityGuangzhouGuangdong 510006China Department of General Surgery(Colorectal Surgery) The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510655China Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510655China Biomedical Innovation Center The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510655China Shanghai Artificial Intelligence Laboratory Shanghai 200232China

Background:The goal of the assisted reproductive treatment is to transfer one euploid blastocyst and to help infertile women giving birth one healthy *** algorithms have been used to assess the ploidy status of embryos derived from couples with normal chromosome,who subjected to preimplantation genetic testing for aneuploidy(PGT-A)***,it is currently unknown whether artificial intelligence model can be used to assess the euploidy status of blastocyst derived from populations with chromosomal ***:From February 2020 to May 2021,we collected the whole raw time-lapse videos at multiple focal planes from in vitro cultured embryos,the clinical information of couples,and the comprehensive chromosome screening results of those blastocysts that had received PGT ***,we developed a novel deep learning model called the Attentive Multi-Focus Selection Network(AMSNet)to analyze time-lapse videos in real time and predict blastocyst *** upon AMSNet,we integrated additional clinically predictive variables and created a second deep learning model,the Attentive Multi-Focus Video and Clinical Information Fusion Network(AMCFNet),to assess the euploidy status of *** efficacy of the AMCFNet was further tested in embryos with parental chromosomal *** receiver operating characteristic curve(ROC)was used to evaluate the superiority of the ***:A total of 4112 embryos with complete time-lapse videos were enrolled for the blastocyst formation prediction task,and 1422 qualified blastocysts received PGT-A(n=589)or PGT for chromosomal structural rearrangement(PGT-SR,n=833)were enrolled for the euploidy assessment task in this *** AMSNet model using seven focal raw time-lapse videos has the best real-time *** real-time accuracy for AMSNet to predict blastocyst formation reached above 70%on the day 2 of embryo culture,and then increased to 80%on the day 4 of embryo ***

关键词： Euploidy status embryo Time-lapse imaging Deep learning Spatio-temporal analysis Preimplantation genetic testing for chromosomal structural rearrangement

Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development of Marsupenaeus japonicas

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2024年第1期42卷 201-215页

作者： Jing WANG Longjun PU Xiaojuan ZHANG Cuicui LIANG Dandan DONG Jiantao GUAN Huarong GUO Ministry of Education Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

Regulation of microRNAs(miRNAs)on various biological processes has been a surprising and exciting *** of miRNAs is the first step to comprehensively understand their ***,attempts on global identification and functional verification of miRNAs are very limited in penaeid shrimp Marsupenaeus japonicus,an economically important aquatic *** performing an integrated analysis of transcriptomic profile from gastrula embryos of ***,21conserved miRNAs in ***(mja-miRNAs),belonging to 19 miRNA families,were identified and *** the 21 mja-miRNAs,15 miRNAs were successfully verified to be predominantly expressed in gastrula stage,where they displayed dynamic expression patterns compared with those in naupliuin *** on perfect or near-perfect match to target region,120 target genes were predicted at transcriptome-wide ***,gene ontology(GO)classification and metabolic pathway annotation revealed eight targets that were actively involved in developmental *** the predicted miRNA-mRNA pairs,six targets were then randomly selected and experimentally validated by dual luciferase reporter assay,where three pairs were proved with potential targeting ***,to search for conserved miRNAs potentially involved in early development of ***,we combined in silico and experimental methods,which can be applied in other organisms as *** data implied important roles of miRNAs in the early embryonic development and also suggested the presence of complex miRNA-mRNA functional networks in ***.

关键词： microRNAs(miRNAs) identification Marsupenaeus japonicus embryo transcriptome