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The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting elf3 for degradation in Arabidopsis

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Journal of Integrative Plant Biology 2021年第6期63卷 1097-1103页

作者： Lin-Lin Zhang Wei Li Ying-Ying Tian Seth Jon Davis Jian-Xiang Liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China Department of Biology University of YorkHeslingtonYork YO105DDUK State Key Laboratory of Crop Stress Biology School of Life SciencesHenan UniversityKaifeng 475004China

Plants are capable of coordination of their growth and development with ambient temperatures.EARLY FLOWERING3(elf3), an essential component of the plant circadian clock, is also involved in ambient temperature sensing, as well as in inhibiting the expression and protein activity of the thermoresponsive regulator phytochrome interacting factor4(PIF4). The elf3 activity is subjected to attenuation in response to warm temperature;however,how the protein level of elf3 is regulated at warm temperature remains less understood. Here, we report that the E3 ligase XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA, XBAT35, mediates elf3 degradation. XBAT35 interacts with elf3 and ubiquitinates elf3. Loss-of-function mutation of XBAT35 increases the protein level of elf3 and confers a short-hypocotyl phenotype under warm temperature conditions. Thus, our findings establish that XBAT35 mediates elf3 degradation to lift the inhibition of elf3 on PIF4 for promoting thermoresponsive hypocotyl growth in plants.

关键词： The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting elf3 for degradation in Arabidopsis elf

First-Principles Calculations of Elastic Constants of Superconducting MgB2

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Chinese Physics Letters 2005年第7期22卷 1764-1767页

作者：郭华忠陈向荣朱俊蔡灵苍高洁 InstituteofAtomicandMolecularPhysics SichuanUniversityChengdu610065//InternationalCentreforMaterialsPhysicsChineseAcademyofSciencesShenyang110016 InstituteofAtomicandMolecularPhysics SichuanUniversityChengdu610065 CollegeofPhysicalScienceandTechnology SichuanUniversityChengdu610065 LaboratoryforShockWaveandDetonationPhysicsResearch InstituteofFluidPhysicsChineseAcademyofEngineeringPhysicsMianyang621900

The five independent elastic constants of superconducting MgB2 are obtained using the first-principles plane wave method with the new relativistic analytic pseudopotential of the Hartwigsen–Goedecker–Hutter (HGH) scheme in the frame of local density approximation. The dependences of bulk modulus on temperature and pressure are also obtained. It is suggested that the HGH-type pseudopotentials are successful in investigating the ground-state mechanical properties of any solids.

关键词： PDOS DOS elf

Theoretical Study of Interaction between Apalutamide Anticancer Drug and Thymine by DFT Method

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Chinese Journal of Structural Chemistry 2019年第10期38卷 1645-1663,1609页

作者： SIYAMAK Shahab MASOOME Sheikhi MEHRNOOSH Khaleghian SHAMSA Sharifi SADEGH Kaviani Institute of Physical Organic Chemistry National Academy of Sciences of Belarus13 Surganov StrMinsk 220072Republic of Belarus Institute of Chemistry of New Materials National Academy of Sciences of Belarus36 Skarina Str.Minsk 220141Republic of Belarus Belarusian State University IS El BSUMinskRepublic of Belarus Young Researchers and Elite Club Gorgan BranchIslamic Azad UniversityGorganIran Young Researchers and Elite Club Islamshahr BranchIslamic Azad UniversityIslamshahrIran Department of Chemistry Robat Karim BranchIslamic Azad UniversityRobat KarimIran Department of Chemistry Ferdowsi University of MashhadMashhadIran

The main purpose of this study is a better comprehension of the non-bonded interaction between an anticancer drug apalutamide and deoxyribonucleic acid(DNA).In the prese nt work,the in teraction between an ticancer drug apalutamide and one of the DNA bases called 2,-deoxythymidine 5,-monophosphate(thymine)by Density Functional Theory(DFT)calculations in the solvent water has been investigated for the first time.The non-bonded interaction effects of the molecule apalutamide with thymine on the electronic properties,chemical shift tensors and natural charges have been also detected.The natural bond orbital(NBO)analysis was performed for determining the role of electron donor and acceptor of the molecules apalutamide and thymine at the complex thymine/apalutamide.Both Electron location function(elf),localized orbital locator(LOL)and quantum theory of atoms in molecules(QTAIM)analysis were carried out in order to determine the chemical bond nature in the investigated compounds.The values of elf and LOL parameters for the selected bonds are small,which confirms the non-covalent character of these bonds in nature.The electronic spectra of the apalutamide drug,thymine and complex thymine/apalutamide in solvent water were calculated by Time Dependent Density Functional Theory(TD-DFT)for the investigation of interaction effect;Non-bonded interaction between the compound apalutamide and thymine has changed the value of λmax.

关键词： apalutamide thymine DFT elf NBO

AIM and elf Analyses of Halogen Bonding in NCS ： BrCl Complexes

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Chinese Journal of Chemistry 2011年第11期29卷 2416-2420页

作者：李晓艳孙洁张雪英曾艳丽郑世钧孟令鹏 Institute of Computational Quantum Chemistry College of Chemistry and Material Science Hebei Normal University Shijiazhuang Hebei 050016 China

在五建筑群结合的卤素的性质在 thiocyanate (NCS ) 之间形成了基，一个 BrCl 分子被原子的量子论在这份报纸在分子(QTAIM ) 和电子本地化功能(矮子) 分析。卤素原子的几何学在 N 原子结合了的计算结果表演比那些在 S 原子或 C 原子结... 详细信息

在五建筑群结合的卤素的性质在 thiocyanate (NCS ) 之间形成了基，一个 BrCl 分子被原子的量子论在这份报纸在分子(QTAIM ) 和电子本地化功能(矮子) 分析。卤素原子的几何学在 N 原子结合了的计算结果表演比那些在 S 原子或 C 原子结合的是稳定的。分子的静电的潜力决定建筑群的几何学和稳定性。在捐赠电子的 NCS 激进分子的 S 原子或 N 原子的原子价盆被压缩，它的人口在结合卤素的建筑群的形成的过程期间减少。

关键词： elf NCS 卤素粘接 AIM 分子静电势几何形状硫氰酸盐

Native/Non-native English Speakers’Attitudes:Addressing Learners’Goals and Needs Towards Pronunciation Issues

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Journal of Literature and Art Studies 2022年第1期12卷 87-102页

作者： Anna Maria De Bartolo University of Calabria Rende-CosenzaItaly

The study attempts to explore native and non-native English speakers’attitudes towards accents and pronunciation-related issues.The sample group surveyed is composed of non-native English speakers,specifically,Italian students studying at the University of Calabria(Italy)and native English speakers from Alberta University(Canada)and Florida Atlantic University(USA).An online link to a questionnaire was sent via email to all participants and was used as a research instrument to collect quantitative data.The research questions will investigate learners’attitudes in relation to the following aspects:accent and identity,beliefs about native/non-native accents,impact of pronunciation on communication,and learners’expectations towards pronunciation teaching.Firstly,mean scores in relation to the aforementioned aspects will be examined.Secondly,differences between native/non-native speakers’responses will be statistically analysed.Thirdly,non-native learners’responses will be correlated with their proficiency level in English to identify the extent to which language competence may affect learners’attitudes.The study aims to gain useful insights that may hopefully raise students and teachers’awareness of what models we expect learners to imitate and attain in the English language classroom,how appropriate and relevant these may be especially in the globalized English world where non-native speakers will increasingly use English in a diversity of forms to achieve their communicative goals.The preliminary results will be presented and pedagogical considerations suggested.

关键词： accents and pronunciation elf intercultural communication native/non-native English speakers attitudes

Cloning and identification of magnetic field-responsive genes in Daudi cells

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Chinese Science Bulletin 2000年第11期45卷 1006-1010页

作者： Ruiying Wu Huai Jiang Genlin Hu Yiti Fu Deqiang Lu Zhejiang Univ Sch Med Microwave Inst Hangzhou 310006 Peoples R China

In order to study the molecular mechanisms of elf MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 ml 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has been verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a novel gene in human cell which is induced by elf MF.

关键词： elf magnetic field mRNA differential display responsive genes.

Establishment of the elf Network in Yunnan and Electromagnetic Precursory Monitoring Results of the Yangbi MS5.1 Earthquake on March 27,2017

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Earthquake Research in China 2018年第2期32卷 254-264页

作者： Zhang Ping Zhao Guoze Tang Ji Liu Feng Sun Weihuai Han Bing Wang Lifeng Zhang Li Yunnan Earthquake Agency Kunming 650224China Institute of Geology China Earthquake AdministrationBeijing 100029China

This paper summarizes the layout of the Yunnan seismic elf electromagnetic observation network,site selection,elf electromagnetic instrument system,data processing and other construction. The principle and method of using the elf electromagnetic wave technique to monitor and predict earthquakes are expounded. The long term monitoring of elf electromagnetic fields is carried out in the Yunnan earthquake prone area,and at the same time,the changes in electrical parameters and spatial electromagnetic fields of the regional crustal medium structure are monitored. The functions such as automatic,quasi real time, remote monitoring, network monitoring, data processing specialization, data service,data sharing and industrialization of the elf electromagnetic observation data have been realized. In order to capture the deep electromagnetic precursory information of the earthquakes,service for earthquake prediction research,which has broad application prospects and development potential. Through the research of the seismicity of Yunnan in the trial run period of the project,the preliminary results of the extreme low frequency electromagnetic observation of the Yunnan earthquake in recent years are given. The electromagnetic precursors and the electromagnetic effects of the Yangbi earthquake are recorded. In the 3-month period before the earthquake,the power spectrum of the electric and magnetic fields,the apparent resistivity and the impedance phase in the observed signals are all abnormal,and gradually increased with time. The maximum value is reached 20 days before the earthquake,and an earthquake occurs when the change is restored to normal.

关键词： elf Seismic electromagnetic network Earthquake precursor Abnormal Yunnan

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张衡一号卫星观测的电离层嘶声波动研究

张衡一号卫星观测的电离层嘶声波动研究

作者：吕访贤应急管理部国家自然灾害防治研究院

学位级别：硕士

科学家们基于国际和国内的多颗电磁科学卫星翔实的观测,开展了大量的理论和模拟研究,证实了孕震过程中释放的地球物理场、地球化学多参量信号,诸如电磁异常、物质、能量、地球化学信号等,会通过岩石圈-大气层-电离层-磁层不同圈层之间... 详细信息

科学家们基于国际和国内的多颗电磁科学卫星翔实的观测,开展了大量的理论和模拟研究,证实了孕震过程中释放的地球物理场、地球化学多参量信号,诸如电磁异常、物质、能量、地球化学信号等,会通过岩石圈-大气层-电离层-磁层不同圈层之间耦合作用,传播到近地空间电离层,甚至远至磁层。这为从空间信息识别和提取诸如地震的自然灾害前兆信息以及风险监测带来了新的方案。我国积极探索地震预测新方法新技术,我国自主研制的首颗用于地震电磁监测的试验卫星张衡一号01星于2018年2月2日成功发射入轨,卫星轨道高度为507km,倾角97.4°。该卫星目前已经稳定在轨运行5年多,积累了电磁场、等离子体参数及能量粒子的翔实观测数据。张衡一号电磁卫星运行的轨道空间充满了各种类型的扰动,尤其在elf(Exetremly Low Frequency)频段的电离层嘶声现象非常普遍,即使在地球磁场宁静情况下elf嘶声波动强度远比其他类型的电磁波动要活跃很多。可以认为elf嘶声波动是电离层/等离子体层空间内的一种背景电磁波动。然而前人工作发现在这个空间里卫星观测的地震电离层异常也多半出现在elf频段上,也可激发与嘶声类似的电磁扰动。因此如何在elf频段客观、准确提取地震异常信息,需要对这个轨道空间的elf电离层嘶声开展深入研究。此乃本文立题依据。本论文重点利用张衡一号电磁卫星的elf频段的电磁场波形和频谱数据重点研究了 elf频段的电离层嘶声现象。重点使用张衡一号01星自2018年开始积累的中国内陆强震期间的所有数据,通过频谱特征分析和奇异值波矢量分解(SVD)研究了记录到的强震发生期间孕震时空窗口内的电离层嘶声事件的波传播参数,统计分析了地震-电离层嘶声的传播特征。其次,本文尝试使用时域有限差分(FDTD)的电磁计算方法,对波的传播过程进行数值模拟研究。具体研究结果如下:1.构建了一套地震-电离层嘶声研究的观测数据集本文利用数据预处理的方法挑选出张衡一号卫星在中国地区所有与地震-电离层嘶声相关的电磁异常事件,分析了地震-电离层时空相关性以及空间天气事件的影响。本文将所有记录与地震-电离层嘶声事件有关的轨道数据存放一起,为了降低后续数据计算的成本,本文按照时间和频率截取了所有轨道上嘶声波结构的特定轨道,构建了一套地震-电离层嘶声事件观测数据集。2.下行电离层嘶声传播及分布特征本文研究发现电离层嘶声的频谱呈现出无结构的特征,波矢量特征大多数呈现向下传播,大多数波的法向极角出现在-180°。尤其对沿着质子回旋分布的电离层嘶声波PCBEs的特性研究发现,PCBEs的带宽随磁纬的降低而逐渐减小,且存在明显的季节规律性。在诸多下行的电离层嘶声事件中,我们找到了少部分上行的电离层嘶声事件,两者常常混杂在一起。3.地震期间上行电离层嘶声现象及统计特征本文利用SVD算法对数据集中每个事件计算出波的方位角、法向极角、平面度、椭圆极化率等关键传播参数。根据波传播方位角的大小来判断波的传播方向,传播方位角接近于0°则表示波从地球方向向上传播。对所有向上传播的波的传播参数进行统计发现,地震期间电离层嘶声大多出现在300 Hz至800Hz的频带范围,大多数波的法向极角出现在40°至60°之间,方位角主要在靠近0°约60°的范围内,椭圆极化率则表现出更复杂的特征,变化在±0.5左右,平面度主要在0.5到1之间占主导且峰值集中在0.8到0.9之间。4.利用电磁计算方法FDTD尝试研究elf频段波传播过程本文初步建立elf波在地球-电离层传播的数值模型。基于该模型简单模拟了从地面出发的垂直入射波到电离层高度的波传播过程,利用FDTD迭代初步计算了随时间、空间高度变化的电磁场分布。随着离子浓度随高度的变化,垂直入射波从近地面传播到电离层时大部分波被吸收、反射,只有一小部分波穿透电离层到达卫星轨道空间,该信号可以被卫星获取。

关键词：张衡一号 elf 电离层嘶声地震波矢量时域有限差分

基于微内核的动态加载与链接技术的研究与实现

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基于微内核的动态加载与链接技术的研究与实现

作者：陈阔电子科技大学

学位级别：硕士

随着计算机软硬件技术的发展,计算机设备朝着网联化、智能化、综合化不断发展,这要求操作系统具有更高的安全可靠性及可扩展性,主流的宏内核操作系统及嵌入式操作系统难以满足这样的需求。微内核操作系统通过精简内核设计,将绝大部分服... 详细信息

随着计算机软硬件技术的发展,计算机设备朝着网联化、智能化、综合化不断发展,这要求操作系统具有更高的安全可靠性及可扩展性,主流的宏内核操作系统及嵌入式操作系统难以满足这样的需求。微内核操作系统通过精简内核设计,将绝大部分服务解耦后,以独立组件的方式在用户态运行。这样的设计可以做到高安全可靠性及可扩展性,能很好地适应智能网联汽车等场景。动态加载与链接是通用操作系统的一个重要功能,是软件工程模块化设计的关键支撑要素。但是动态加载与链接在微内核操作系统上的研究与应用较少,在工程实现上,许多微内核操作系统采用在编译时配置选择所需系统服务与应用程序的静态构建方式,缺乏灵活性的同时,也可能破坏系统服务及应用程序之间的隔离性。本文基于elf格式程序文件在Cortex-M和Cortex-A两种平台上研究实现了动态加载与链接技术,提升了微内核操作系统的可扩展性、开发的便捷性、系统服务及应用程序之间的隔离性等。在Cortex-M平台上基于地址无关码实现了对elf程序的动态加载、动态链接、运行时重定位技术,针对随机访问存储器资源紧缺的平台,研究实现了代码与数据分离、共享地址无关码等技术,并对每种技术进行了有针对性和代表性的功能测试验证,提升了Cortex-M平台上微内核操作系统的可扩展性及开发的便捷性,研究实现了解决随机访问存储器资源紧缺的方案。在支持虚拟内存技术的Cortex-A平台上,本文研究实现了对可执行文件、动态链接库、地址无关可执行对象的支持,并在功能测试的基础上,进行了一些开源软件的移植尝试。本文提出的基于微内核的动态加载与链接技术对构建微内核操作系统软件生态具有一定的通用性和持续性。

关键词：微内核动态加载与链接 elf Cortex-M Cortex-A