检索结果-南通市图书馆

Functional analyses of an e3 ligase gene AIP2 from wheat in Arabidopsis revealed its roles in seed germination and pre‐harvest sprouting

引用

Journal of Integrative Plant Biology 2014年第5期56卷 480-491页

作者： Dong-Yao Gao Zhao-Shi Xu Yi He Yong-Wei Sun You-Zhi Ma Lan-Qin Xia Institute of Crop Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement the Chinese Academy of Agricultural Sciences (CAAS) Agricultural and Sideline Base Unit 65426 of the People’s Liberation Army

Pre‐harvest sprouting（PHS） seriously affects wheat yield and quality of the grain. ABI3 is a key factor in the activation of seed development and repression of germination in Arabidopsis. An ABI3‐interacting protein（AIP2） could polyubiquitinate ABI3, impair seed dormancy and promote seed germination in Arabidopsis. In this study,two wheat AIP2 genes, TaAIP2A and TaAIP2B, were *** localization assay and yeast two‐hybrid analysis revealed that TaAIP2A and TaAIP2B may function through interaction with wheat Viviporous‐1（TaVp1）. The transcripts TaAIP2A and TaAIP2B were more abundant in wheat PHS susceptible cultivars than that of resistant ones, and decreased gradually following seed development. expression of TaAIP2A and TaAIP2B in Arabidopsis aip2‐1 mutant lines resulted in earlier flowering, promotion of seed germination,and reduced ABA sensitivity, respectively, somehow mimicking the phenotype of the wild type, with TaAIP2B having a stronger role in these aspects. Furthermore, the expression ofupstream genes ABI1 and ABI2 were upregulated, whereas that of downstream genes ABI3 and ABI5 were downregulated in both TaAIP2A and TaAIP2B complemented lines upon ABA treatment. These results suggested that wheat AIP2s could negatively regulate the ABA signaling pathway and play important roles in seed germination, and thus wheat PHS resistance finally.

关键词： ABA responsiveness AIP2 Arabidopsis e3 ligase functional analysis pre‐harvest sprouting Triticum aestivum L

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two VOZ transcription factors link an e3 ligase and an NLR immune receptor to modulate immunity in rice

引用

Molecular Plant 2021年第2期14卷 253-266页

作者： Jiyang Wang Ruyi Wang Hong Fang Chongyang Zhang Fan Zhang Zeyun Hao Xiaoman You Xuetao Shi Chan Ho Park Kangyu Hua Feng He Maria Bellizzi Kieu Thi Xuan Vo Jong-Seong Jeon Yuese Ning Guo-Liang Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Department of Plant Pathology Ohio State UniversityColumbusOH 43210USA Graduate School of Biotechnology and Crop Biotech Institute Kyung Hee UniversityYongin 17104Korea

Nucleotide-binding leucine-rich repeat(NLR)proteins play critical roles in plant ***,how NLRs are regulated and activate defense signaling is not fully *** rice(Oryza sativa)NLR receptor Piz-t confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae and the RING-type e3 ligase AVRPIZ-T INTeRACTING PROTeIN 10(APIP10)negatively regulates Piz-t *** this study,we found that APIP10 interacts with two rice transcription factors,VASCULAR PLANT ONeZINC FINGeR 1(OsVOZ1)and OsVOZ2,and promotes their degradation through the 26S proteasome ***1 displays transcriptional repression activity while OsVOZ2 confers transcriptional activation activity in *** osvoz1 and osvoz2 single mutants display modest but opposite *** resistance in the non-Piz-t ***,the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death,and silencing of both genes via RNA interference also leads to dwarfism,mild cell death,and enhanced resistance to *** in the non-Piz-t *** OsVOZ1 and OsVOZ2 interact with *** silencing of OsVOZ1 and OsVOZ2 in the Piz-t background decreases Piz-t protein accumulation and transcription,reactive oxygen species-dependent cell death,and resistance to *** containing *** together,these results indicate that OsVOZ1 and OsVOZ2 negatively regulate basal defense but contribute positively to Piz-t-mediated immunity.

关键词： rice e3 ligase VOZ transcription factor NLR protein fungal pathogen Magnaporthe oryzae cell death

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type e3 ligase during legume nodulation

引用

Molecular Plant 2024年第7期17卷 1090-1109页

作者： Hao Li Yajuan Ou Jidan Zhang Kui Huang Ping Wu Xiaoli Guo Hui Zhu Yangrong Cao National Key Lab of Agricultural Microbiology Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China National Key Lab of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China

The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands;however,such mechanisms regulating nodulation factor(NF)receptor(NFR)-mediated perception of NFs to establish symbiosis remain *** this study,we unveil the pivotal role of the NFR-interacting RING-type e3 ligase 1(NIRe1)in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus *** demonstrated that NiRe1 has a dual function in this regulatory *** associates with both NFR1 and NFR5,facilitating their degradation through K48-linked polyubiquitination before rhizobial ***,following rhizobial inoculation,NFR1 phosphorylates NIRe1ata conserved residue,Tyr-109,inducing a functional switch in NIRe1,which enables NIRe1tomediateK63-linkedpolyubiquitination,thereby stabilizing NFR1/NFR5 in infected root *** introduction of phospho-dead NIRe1Y1osF leads to delayed nodule development,underscoring the significance of phosphorylation at Tyr-1o9 in orchestrating symbiotic ***,expression of the phospho-mimic NIRe1Y0e results in the formation of spontaneous nodules in ***,further emphasizing the critical role of the phosphorylation-dependent functional switch in *** summary,these findings uncover a fine-tuned symbiotic mechanism that a single e3 ligase could undergo a phosphorylationdependent functional switch to dynamically and precisely regulate NF receptor protein levels.

关键词： nodule symbiosis nodulation factor receptors e3 ligase ubiquitination phosphorylation protein degradation protein stabilization Lotus japonicus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The RING e3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice

引用

Molecular Plant 2021年第10期14卷 1699-1713页

作者： Wensi Yang Kun Wu Bo Wang Huanhuan Liu Siyi Guo Xiaoyu Guo Wei Luo Shengyuan Sun Yidan Ouyang Xiangdong Fu Kang Chong Qifa Zhang Yunyuan Xu Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan) Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China University of Chinese Academy of Sciences Beijing 100049China The State Key Laboratory of Plant Cell and Chromosome engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Innovation Academy for Seed Design CASBeijing 100101China

G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice,but how these pathways are coordinated in regulating this process is ***,we show that Chang Li Geng 1(CLG1),which encodes an e3 ligase,regulates grain size by targeting the Gγprotein GS3,a negative regulator of grain length,for *** of CLG1 led to increased grain length,while overexpression of mutated CLG1 with changes in three conserved amino acids decreased grain *** found that CLG1 physically interacts with and ubiquitinats GS3which is subsequently degraded through the endosome degradation pathway,leading to increased grain ***,we identified a critical SNP in the exon 3 of CLG1 that is significantly associated with grain size variation in a core collection of cultivated *** SNP results in an amino acid substitution from Arg to Ser at position 163 of CLG1 that enhances the e3 ligase activity of CLG1 and thus increases rice grain *** the expression level of CLG1 and the SNP CLG1163S may be useful variations for manipulating grain size in rice.

关键词： grain size CLG1 GS3 e3 ligase endosome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MYB106 is a negative regulator and a substrate for CRL3^(BPM) e3 ligase in regulating flowering time in Arabidopsis thaliana

引用

Journal of Integrative Plant Biology 2021年第6期63卷 1104-1119页

作者： Liu Hong Fangfang Niu Youshun Lin Shuang Wang Liyuan Chen Liwen Jiang Center for Cell&Developmental Biology State Key Laboratory of AgrobiotechnologySchool of Life SciencesThe Chinese University of Hong KongShatinNew TerritoriesHong KongChina Shenzhen Research Institute The Chinese University of Hong KongShenzhen 518057China Shenzhen Technology University Shenzhen 518000China School of Chemical Biology&Biotechnology Peking University Shenzhen Graduate SchoolNanshan DistrictShenzhen 518055China

Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post-translational regulation of the activities of transcription factors(TFs) is a dynamic and essential mechanism for plant growth and development. CRL3 BPMe3 ligase is a CULLIN3-based e3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over-expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106-1 mutants, including Flowering Locus T(FT) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays(eMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction(ChIP-q PCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3 BPMe3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3 BPM e3 ligases in Arabidopsis.

关键词： flowering time MYB transcription factor e3 ligase CUL3~(BPM)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

TaGW2L,a GW2-like RING finger e3 ligase,positively regulates heading date in common wheat(Triticum aestivum L.)

引用

The Crop Journal 2022年第4期10卷 972-979页

作者： Daijing Zhang Xiaoxu Zhang Wu Xu Tingting Hu Jianhui Ma Youfu Zhang Jian Hou Chenyang Hao Xueyong Zhang Tian Li College of Life Sciences Henan Normal UniversityXinxiang 453007HenanChina Key Laboratory of Crop Gene Resources and Germplasm enhancement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

RING finger e3 ligases play an important role in regulating plant growth and development by mediating substrate *** this study,we identified TaGW2L,encoding a Grain width and weight2(GW2)-like RING finger e3 ligase,as a novel positive regulator of heading date in wheat(Triticum aestivum L.).TaGW2L exhibited high amino acid sequence similarities with TaGW2 homoeologs,particularly in the conserved RING finger *** analysis indicated that TaGW2L was constitutively expressed in various wheat ***2L showed transactivation activity in yeast and could interact with the ubiquitin-conjugating enzymes e2_(s).An in vitro ubiquitination assay verified that TaGW2L possessed a similar e3 ligase activity to *** of the TaGW2L-7A homoeolog in wheat led to a significantly earlier heading date under both natural conditions and long-day *** analysis revealed that multiple known genes positively regulating wheat heading were significantly upregulated in the TaGW2L-7A-overexpression plants compared with the wild-type ***,our findings shed light on the role of TaGW2L in wheat heading date and provide potential applications of TaGW2L for the adaptation improvement of crops.

关键词： TaGW2L RING finger domain e3 ligase Heading date Triticum aestivum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Ubiquitin e3 ligase PUB17 Positively Regulates Immunity by Targeting a Negative Regulator, KH17, for Degradation

引用

Plant Communications 2020年第4期1卷 75-86页

作者： Hazel McLellan Kai Chen Qin He Xintong Wu Petra C.Boevink Zhendong Tian Paul R.J.Birch Division of Plant Science School of Life ScienceUniversity of Dundee(at JHI)InvergowrieDundee DD25DAUK Key Laboratory of Horticultural Plant Biology(HZAU) Ministry of EducationKey Laboratory of Potato Biology and Biotechnology(HZAU)Ministry of Agriculture and Rural AffairsHuazhong Agricultural UniversityWuhanHubei 430070China Cell and Molecular Science James Hutton InstituteInvergowrieDundee DD25DAUK

Ubiquitination is a post-translational modification that regulates many processes in *** ubiquitin e3 ligases act as either positive or negative regulators of immunity by promoting the degradation of different ***17 is an e3 ligase that has previously been shown to positively regulate immunity to bacteria,fungi and oomycetes,including the late blight pathogen Phytophthora *** of StPUB17 promotes pathogen colonization and attenuates Cf4/avr4 cell *** yeast-2-hybrid and co-immunoprecipitation we identified the putative K-homology(KH)RNA-binding protein(RBP),StKH17,as a candidate substrate for degradation by ***17 acts as a negative regulator of immunity that promotes *** infection and suppresses specific immune pathways.A KH RBP domain mutant of StKH17(StKH17GDDG)is no longer able to negatively regulate immunity,indicating that RNA binding is likely required for StKH17 *** StPUB17 is a known target of the ubiquitin e3 ligase,StPOB1,we reveal an additional step in an e3 ligase regulatory cascade that controls plant defense.

关键词： oomycete plant disease late blight e3 ligase KH RNA-binding protein

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

eCD Promotes Gastric Cancer Metastasis by Blocking e3 ligase ZFP91-Mediated hnRNP F Ubiquitination and Degradation

ECD Promotes Gastric Cancer Metastasis by Blocking E3 Ligase...

引用

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青...

作者：徐松辉晏光荣 Biomedicine Research Center the Third Affiliated Hospital of Guangzhou Medical University

The human ortholog of the Drosophila ecdysoneless gene(eCD) is required for embryonic development and cell cycle progression;however, its role in cancer progression and metastasis remains unclear. Here, we found that eCD is frequently overexpressed in gastric cancer(GC), especially in metastatic GC, and is correlated with poor clinical outcomes in GC patients. Silencing eCD inhibited GC migration and invasion in vitro and metastasis in vivo, while eCD overexpression promoted GC migration and invasion. eCD promoted GC invasion and metastasis by protecting hnRNP F from ubiquitination and degradation. We identified ZFP91 as the e3 ubiquitin ligase that is responsible for hnRNP F ubiquitination at Lys 185 and proteasomal degradation. eCD competitively bound to hnRNP F via the N-terminal STG1 domain(13-383 aa), preventing hnRNP F from interacting with ZFP91, thus preventing ZFP91-mediated hnRNP F ubiquitination and proteasomal degradation. Collectively, our findings indicate that eCD promotes cancer invasion and metastasis by preventing e3 ligase ZFP91-mediated hnRNP F ubiquitination and degradation, suggesting that eCD may be a marker for poor prognosis and a potential therapeutic target for GC patients.

关键词： gastric cancer metastasis eCD e3 ligase ubiquitination

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chemical tools for e3 ubiquitin ligase study

引用

Chinese Chemical Letters 2023年第4期34卷 67-74页

作者： Yangwode Jing Chong Zuo Yun-Xiang Du Junxiong Mao Ruichao Ding Jiachen Zhang Lu-Jun Liang Qian Qu Institute of Translational Medicine Shanghai Jiao Tong UniversityShanghai 200240China Tsinghua-Peking Center for Life Sciences Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084China

e3 ubiquitin ligases catalyze the final step of ubiquitylation,a crucial post-translational modification involved in almost every process in eukaryotic cells.e3 ubiquitin ligases are key regulators of cellular events,and the investigation into their functions and functioning mechanisms are research areas with great *** or semi-synthetic tools have greatly facilitated the research about the enzyme activity,distribution in different physiological events,and catalytic mechanism of e3 ubiquitin *** this review,we summarize the development of chemical tools for e3 ubiquitin ligases with an emphasis on the synthetic *** show the utility of these chemical tools by briefly discussing their applications in biological research.

关键词： Chemical tools Protein chemical synthesis Ubiquitylation e3 ligase enzymatic mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Arabidopsis U-box e3 ubiquitin ligase PUB11 negatively regulates drought tolerance by degrading the receptor-like protein kinases LRR1 and KIN7

引用

Journal of Integrative Plant Biology 2021年第3期63卷 494-509页

作者： Xuexue Chen Tingting Wang Amin Ur Rehman Yu Wang Junsheng Qi Zhen Li Chunpeng Song Baoshan Wang Shuhua Yang Zhizhong Gong State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China Collaborative Innovation Center of Crop Stress Biology Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China Key Lab of Plant Stress Research College of Life ScienceShandong Normal UniversityJi'nan 250000China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding 100193China

Both plant receptor-like protein kinases(RLKs)and ubiquitin-mediated proteolysis play crucial roles in plant responses to drought ***,the mechanism by which e3 ubiquitin ligases modulate RLKs is poorly *** this study,we showed that Arabidopsis PLANT U-BOX PROTeIN 11(PUB11),an e3 ubiquitin ligase,negatively regulates abscisic acid(ABA)-mediated drought ***11 interacts with and ubiquitinates two receptor-like protein kinases,LeUCINe RICH RePeAT PROTeIN 1(LRR1)and KINASe 7(KIN7),and mediates their degradation during plant responses to drought stress in vitro and in ***11 mutants were more tolerant,whereas Irr1 and kin7 mutants were more sensitive,to drought stress than the wild *** analyses show that the pub11 Irr1 kin7 triple mutant exhibited similar drought sensitivity as the Irr1 kin7 double mutant,placing PUB11 upstream of the two *** acid and drought treatment promoted the accumulation of PUB11,which likely accelerates LRR1 and KIN7 ***,our results reveal that PUB11 negatively regulates plant responses to drought stress by destabilizing the LRR1 and KIN7 RLKs.

关键词： abscisic acid drought response e3 ligase receptor-like kinase ubiquitination

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：