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Multifaceted roles of ASB proteins and its pathological significance

Frontiers in Biology 2018年第5期13卷 376-388页

作者： Vivek Vishnu Anasa Palaniyandi Ravanan Priti Talwar Apoptosis and Cell Survival Research Lab Department of Biosciences School of Biosciences and Technology VIT University Vellore Tamil Nadu 632014 lndia

BACKGROUND： Post-translational （PT） modification in cells regulates many intracellular events like signal transduction, transcription, cell cycle, protein quality control, apoptosis and cellular development. Ubiquitination is one of the PT modifications which ftmctions as a marker for degradation of target proteins by the proteasome and as a regulatory mechanism for several signalling pathways. The ubiquitination mechanism requires multiple enzymes, including el, e2, and e3 ligases. Among them, e3 ligases play a major role in recognizing target proteins and an essential feature of protein homeostatic mechanisms within the cell. Most of the ASB （ankyrin repeat SOCS box） proteins fimction as RING family of e3 ubiquitin ligases characterized by the presence of two conserved domains N-terminal ankyrin repeat and C-terminal SOCS box domain MeTHODS and ReSULTS： Current studies have shown that some ASBs function as important regulators of several signalling pathways. This review gives an overview of ASB proteins on numerous cellular processes such as insulin signalling, spermatogenesis, myogenesis and in cellular development. Including various pathological situations, such as cancer, primary open-angle glaucoma, and inflammation, indicating that ASBs has important functions in both normal and pathological development CONCLUSIONS： This article provides a precise comprehensive focus on ASBs protein structure, its biological functions, and their pathological significance.

关键词： ankyrin repeat SOCS box e3 ligase cancer spermatogenesis cellular development

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Regulation of inflammasomes by ubiquitination

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Cellular & Molecular Immunology 2016年第6期13卷 722-728页

作者： Joseph S Bednash Rama K Mallampalli Division of Pulmonary Allergy and Critical Care Medicine Department of Medicine Acute Lung injury Center of Excellence University of Pittsburgh Pittsburgh PA 15213 USA Department of Cell Biology and Physiology and Bioengineering University of Pittsburgh Pittsburgh PA 15213 USA Medical Specialty Service Line Veterans Affairs Pittsburgh Healthcare System Pittsburgh PA 15240 USA

Inflammasomes are multi-protein complexes that regulate the innate immune response by facilitating the release of inflammatory cytokines in response to pathogen exposure or cellular damage. Pro-inflammatory inflammasome signaling is vital to host defense and helps initiate the process of tissue repair following an insult to the host, but can be injurious, when excessive or chronic. As such, inflammasome activity is tightly regulated. Here we discuss one critical mechanism of inflammasome regulation, ubiquitination, that functions as a universal modulator of protein stability and trafficking. Recent studies have provided important insights into the regulation of inflammasome activation by protein ubiquitination. We review the molecular regulation of inflammasome function, specifically, as it relates to ubiquitination, and discuss the implications for the development of theraoeutics to soecificallv target aberrant inflammasome signaling.

关键词： deubiquitinase e3 ligase inflammasome innate immunity ubiqutin

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FBXL4 serves as a clock output molecule to regulate sleep through promoting GABAA receptor degradation

FBXL4 serves as a clock output molecule to regulate sleep th...

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中国神经科学学会第十二届全国学术会议

作者： Qian LI Yi LI Xiao WANG Junxia QI Huawei TONG Zikai ZHOU Zi Chao ZHANG Junhai HAN Institute of Life Sciences the Key Laboratory of Developmental Genes and Human Disease Southeast University

Sleep is tightly governed by the circadian clock that contains a negative transcriptional feedback loop and synchronizes the physiology and behavior of most animals to daily environmental oscillations. In both vertebrates and invertebrates, the status of sleep and wake is controlled by the electrical activity of pacemaker neurons, which is circadian-regulated and suppressed by inhibitory GABAergic inputs. However, how the circadian clock rhythmically regulates the activity of pacemaker neuron is unclear. Here, we show that the level of Drosophila GABA receptor Resistant to Dieldrin(RDL) undergoes a daily cycle in wake-promoting large ventral lateral neurons(l-LNvs) and identify the e3 ligase Fbxl4 as a clock output molecule promoting RDL poly-ubiquitination and degradation. Depletion of Fbxl4 results in RDL accumulation, shortened sleep onset, and increased sleep time both in the day and the night. Moreover, we reveal that the expression of Fbxl4 in l-LNvs is Clock dependent but fbxl4 mutants exhibit normal locomotor rhythms. Thus, our study uncovers the critical molecular linkage between circadian clock and the electrical activity of pacemaker neurons and demonstrates that clock-dependent Fbxl4 expression downregulates GABAA receptor to increase the activity of pacemaker neurons and promote wakefulness.

关键词： Sleep circadian clock GABAA receptor e3 ligase Fbxl4 degradation

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Ubiquitination and stress signaling

Ubiquitination and stress signaling

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2006中国植物细胞发育与分子生物学学术研讨会

作者：谢旗 Institute of Genetics and Developmental Biology Chinese Academy of Science Beijing China

＜正＞Ubiquitination has been demonstrated to play very important roles in plant hormone signal transduction. Recently, AFP and AIP2 promote ABI5 and ABI3 degradation, respectively have been found to be crucial in ABA signaling pathway. While AtCHIP1 was demonstrated to be an U-bos protein to mono-ubiquitinate PP2A to stabilize it in ABA signaling pathway. Here we show two different RING-finger e3 ligases, AtHOS1 and AtSIR1are involved in different stress signal transduction. HOS1 a RING-variant e3 ligase was demonstrated to be a functional e3 ligase both in vitro and in vivo assays. It negatively regulates a key transcriptional factor ICe1 and play important role in cold stress signaling. While microarray analysis has demonstrated that AtRINGF1 is a salt inducible gene. The expression of AtRINGF1 was detected in all tissues of Arabidopsis by RT-PCR. AtRINGF1 expression is up-regulated by drought and NaCl, but not by cold and ABA. Transgenic Arabidopsis plants carrying AtRINGF1 promoter fused to GUS gene confirmed results above, in particular, the strong induction of GUS expression was found in stomatal guard cells and leaf mesophyll cells under drought stress. AtRINGF1 was tested in an in vitro ubiquitination experiment and the result demonstrated that AtRINGF1 is an e3 ubiquitin ligase. Overexpression of AtRINGF1, conferred ABA-associated phenotypes, such as ABA hypersensitivity, salt hypersensitivity in germination, reduced water loss, partial stomatal closure, enhanced ABA-induced stomata closing and enhanced drought tolerance. The results suggest that AtRINGF1 is involved in drought response by regulating ABA signaling.

关键词： ABA Ubiquitination e3 ligase drought stress cold signal Stomata

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Regulation of Ubiquitin-like with PHD and RING Finger Domain 1(UHRF1) Protein Stability by Heat Shock Protein 90 Chaperone Machinery

Regulation of Ubiquitin-like with PHD and RING Finger Domain...

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中国生物化学与分子生物学会2016年全国学术会议

作者： Guangjin Ding Peilin Chen Hui Zhang Xiaojie Huang Yi Zang Jiwen Li Jia Li Jiemin Wong Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life Sciences East China Normal University National Center for Drug Screening State Key Laboratory of Drug ResearchShanghai Institute of Materia Medica Chinese Academy of Sciences

As a protein critical for the maintenance of DNA methylation and cell proliferation,UHRF1 is frequently highly expressed in various human cancers and is considered as a drug target for cancer *** a high throughput screening for small molecules that induce UHRF1 protein degradation,we have identified the Hsp90 inhibitor 17-allylamino-17-

关键词： UHRF1 HSP90 HSP70 Client Protein 17-AAG(17-allylamino-17-demethoxygeldanamycin) Protein Stability e3 ligase

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Smurfl functions as a thioester bond intermediate-type Nedd8 ligase to promote colorectal cancer

Smurfl functions as a thioester bond intermediate-type Nedd8...

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细胞—生命的基础——中国细胞生物学学会2013年全国学术大会·武汉

作者： Ping Xie Minghua Zhang Shan He Guichun Xing Yu Cui Yu Cao Yawen Tan Nan Chai Jiawei Wu Haiteng Deng Jing Nie Fuchu He Ling qiang Zhang State Key Laboratory of Proteomics Beijing Proteome Research CenterBeijing Institute of Radiation Medicine Department of Clinical Pharmacology and Pharmacy Center of PharmacyChinese PLA General Hospital School of Life Sciences Tsinghua University Department of Molecular Biology Institute of Basic Medical SciencePLA General Hospital Institute of Cancer Stem Cell Dalian Medical University

Neddylation is essential for the viability of most model organisms and involved in cancer development. In contrast with more than 600 ubiquitin ligases are known,less than ten Nedd8 ligases have been *** of the currently reported Nedd8 ligases are the scaffold-type *** we show that the HeCT ligase Smurfl(Smad ubiquity lation regulatory factor 1) functions as a thioester bond intermediate-type Nedd8 *** interacts with Ubc12 and forms a Nedd8-thioester intermediate to catalyze its autoneddylation,resulting in an increase in its ubiquitin ligase activity.

关键词： neddylation e3 ligase Nedd8 ubiquitin Smurf1 colorectal cancer

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